Experiment: KB with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1632 and Psyr_1633 are separated by 42 nucleotides Psyr_1633 and Psyr_1634 overlap by 4 nucleotides Psyr_1634 and Psyr_1635 overlap by 1 nucleotides
Psyr_1632: Psyr_1632 - ComEC/Rec2-related protein:DNA internalization-related competence protein ComEC/Rec2, at 1,835,715 to 1,837,931
_1632
Psyr_1633: Psyr_1633 - MotA/TolQ/ExbB proton channel, at 1,837,974 to 1,838,690
_1633
Psyr_1634: Psyr_1634 - Biopolymer transport protein ExbD/TolR, at 1,838,687 to 1,839,118
_1634
Psyr_1635: Psyr_1635 - lipid-A-disaccharide kinase, at 1,839,118 to 1,840,113
_1635
Position (kb) 1837
1838
1839 Strain fitness (log2 ratio) -2
-1
0
1 at 1836.998 kb on - strand, within Psyr_1632 at 1837.260 kb on + strand, within Psyr_1632 at 1837.260 kb on + strand, within Psyr_1632 at 1837.260 kb on + strand, within Psyr_1632 at 1837.260 kb on + strand, within Psyr_1632 at 1837.261 kb on - strand, within Psyr_1632 at 1837.261 kb on - strand, within Psyr_1632 at 1837.704 kb on - strand, within Psyr_1632 at 1837.704 kb on - strand, within Psyr_1632 at 1837.836 kb on + strand at 1837.836 kb on + strand at 1837.837 kb on - strand at 1837.941 kb on + strand at 1837.973 kb on + strand at 1837.973 kb on + strand at 1837.978 kb on + strand at 1837.978 kb on + strand at 1838.328 kb on + strand, within Psyr_1633 at 1838.328 kb on + strand, within Psyr_1633
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1 vol% remove 1,836,998 - Psyr_1632 0.58 +1.5 1,837,260 + Psyr_1632 0.70 +0.7 1,837,260 + Psyr_1632 0.70 -0.1 1,837,260 + Psyr_1632 0.70 +0.5 1,837,260 + Psyr_1632 0.70 +0.3 1,837,261 - Psyr_1632 0.70 -0.9 1,837,261 - Psyr_1632 0.70 -0.6 1,837,704 - Psyr_1632 0.90 +0.0 1,837,704 - Psyr_1632 0.90 +0.3 1,837,836 + +0.2 1,837,836 + +1.2 1,837,837 - -0.1 1,837,941 + -1.1 1,837,973 + +0.5 1,837,973 + -0.0 1,837,978 + -0.5 1,837,978 + -0.0 1,838,328 + Psyr_1633 0.49 -2.6 1,838,328 + Psyr_1633 0.49 -0.2
Or see this region's nucleotide sequence