Experiment: KB with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1415 and Psyr_1416 are separated by 52 nucleotides Psyr_1416 and Psyr_1417 are separated by 78 nucleotides Psyr_1417 and Psyr_1418 are separated by 171 nucleotides Psyr_1418 and Psyr_1419 are separated by 1 nucleotides
Psyr_1415: Psyr_1415 - TolB, N-terminal:TolB, N-terminal, at 1,600,063 to 1,601,364
_1415
Psyr_1416: Psyr_1416 - OmpA/MotB, at 1,601,417 to 1,601,920
_1416
Psyr_1417: Psyr_1417 - TPR repeat protein, at 1,601,999 to 1,602,745
_1417
Psyr_1418: Psyr_1418 - Radical SAM, at 1,602,917 to 1,603,564
_1418
Psyr_1419: Psyr_1419 - preQ(0) biosynthesis protein QueC, at 1,603,566 to 1,604,255
_1419
Position (kb)
1601
1602
1603 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1601.353 kb on + strand at 1601.404 kb on + strand at 1601.404 kb on + strand at 1601.914 kb on + strand at 1601.918 kb on + strand at 1601.918 kb on + strand at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.241 kb on + strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.242 kb on - strand, within Psyr_1417 at 1602.274 kb on + strand, within Psyr_1417 at 1602.275 kb on - strand, within Psyr_1417 at 1602.389 kb on + strand, within Psyr_1417 at 1602.390 kb on - strand, within Psyr_1417 at 1602.470 kb on + strand, within Psyr_1417 at 1602.471 kb on - strand, within Psyr_1417 at 1602.486 kb on - strand, within Psyr_1417 at 1602.614 kb on + strand, within Psyr_1417 at 1602.614 kb on + strand, within Psyr_1417 at 1602.615 kb on - strand, within Psyr_1417 at 1602.669 kb on + strand, within Psyr_1417 at 1602.669 kb on + strand, within Psyr_1417 at 1602.670 kb on - strand, within Psyr_1417 at 1602.778 kb on - strand at 1602.828 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.894 kb on + strand at 1602.930 kb on + strand at 1602.930 kb on + strand at 1602.993 kb on + strand, within Psyr_1418 at 1603.118 kb on + strand, within Psyr_1418 at 1603.118 kb on + strand, within Psyr_1418 at 1603.119 kb on - strand, within Psyr_1418 at 1603.203 kb on + strand, within Psyr_1418 at 1603.203 kb on + strand, within Psyr_1418 at 1603.204 kb on - strand, within Psyr_1418 at 1603.310 kb on + strand, within Psyr_1418 at 1603.377 kb on - strand, within Psyr_1418 at 1603.407 kb on - strand, within Psyr_1418 at 1603.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1 vol% remove 1,601,353 + +0.2 1,601,404 + -1.6 1,601,404 + -2.2 1,601,914 + -0.3 1,601,918 + -0.6 1,601,918 + -0.7 1,602,241 + Psyr_1417 0.32 +1.1 1,602,241 + Psyr_1417 0.32 -2.4 1,602,241 + Psyr_1417 0.32 +2.3 1,602,241 + Psyr_1417 0.32 -1.1 1,602,242 - Psyr_1417 0.33 -0.6 1,602,242 - Psyr_1417 0.33 -1.1 1,602,242 - Psyr_1417 0.33 -2.3 1,602,242 - Psyr_1417 0.33 -0.4 1,602,242 - Psyr_1417 0.33 -0.3 1,602,242 - Psyr_1417 0.33 -1.8 1,602,242 - Psyr_1417 0.33 -1.0 1,602,242 - Psyr_1417 0.33 -1.3 1,602,242 - Psyr_1417 0.33 -3.3 1,602,242 - Psyr_1417 0.33 -1.7 1,602,242 - Psyr_1417 0.33 -1.4 1,602,242 - Psyr_1417 0.33 -1.6 1,602,242 - Psyr_1417 0.33 -2.1 1,602,274 + Psyr_1417 0.37 -1.3 1,602,275 - Psyr_1417 0.37 -2.8 1,602,389 + Psyr_1417 0.52 -0.3 1,602,390 - Psyr_1417 0.52 +0.4 1,602,470 + Psyr_1417 0.63 -0.5 1,602,471 - Psyr_1417 0.63 -0.3 1,602,486 - Psyr_1417 0.65 -0.3 1,602,614 + Psyr_1417 0.82 +0.5 1,602,614 + Psyr_1417 0.82 +0.0 1,602,615 - Psyr_1417 0.82 -0.1 1,602,669 + Psyr_1417 0.90 +0.7 1,602,669 + Psyr_1417 0.90 -0.5 1,602,670 - Psyr_1417 0.90 -2.0 1,602,778 - -0.5 1,602,828 + +0.3 1,602,894 + +0.0 1,602,894 + -1.2 1,602,894 + -0.3 1,602,894 + -0.6 1,602,930 + +0.1 1,602,930 + -0.3 1,602,993 + Psyr_1418 0.12 -0.3 1,603,118 + Psyr_1418 0.31 +1.2 1,603,118 + Psyr_1418 0.31 -2.2 1,603,119 - Psyr_1418 0.31 +1.0 1,603,203 + Psyr_1418 0.44 -0.3 1,603,203 + Psyr_1418 0.44 -1.1 1,603,204 - Psyr_1418 0.44 -0.3 1,603,310 + Psyr_1418 0.61 -0.6 1,603,377 - Psyr_1418 0.71 -2.7 1,603,407 - Psyr_1418 0.76 -0.8 1,603,565 + +0.4
Or see this region's nucleotide sequence