Experiment: KB with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0826 and Psyr_0827 are separated by 253 nucleotides Psyr_0827 and Psyr_0828 overlap by 4 nucleotides
Psyr_0826: Psyr_0826 - glucose-6-phosphate isomerase, at 934,924 to 936,588
_0826
Psyr_0827: Psyr_0827 - pantothenate synthetase, at 936,842 to 937,693
_0827
Psyr_0828: Psyr_0828 - ketopantoate hydroxymethyltransferase, at 937,690 to 938,490
_0828
Position (kb)
936
937
938 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 935.900 kb on + strand, within Psyr_0826 at 935.943 kb on + strand, within Psyr_0826 at 936.039 kb on - strand, within Psyr_0826 at 936.039 kb on - strand, within Psyr_0826 at 936.221 kb on - strand, within Psyr_0826 at 936.333 kb on - strand, within Psyr_0826 at 936.333 kb on - strand, within Psyr_0826 at 936.581 kb on + strand at 936.582 kb on - strand at 936.605 kb on + strand at 936.605 kb on + strand at 936.687 kb on - strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.762 kb on + strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.970 kb on - strand, within Psyr_0827 at 937.080 kb on + strand, within Psyr_0827 at 937.081 kb on - strand, within Psyr_0827 at 937.161 kb on - strand, within Psyr_0827 at 937.398 kb on + strand, within Psyr_0827 at 937.482 kb on + strand, within Psyr_0827 at 937.482 kb on + strand, within Psyr_0827 at 937.482 kb on + strand, within Psyr_0827 at 937.483 kb on - strand, within Psyr_0827 at 937.531 kb on + strand, within Psyr_0827 at 937.683 kb on - strand at 937.748 kb on + strand at 937.749 kb on - strand at 938.311 kb on - strand, within Psyr_0828 at 938.417 kb on + strand at 938.473 kb on - strand at 938.479 kb on + strand at 938.479 kb on + strand at 938.480 kb on - strand at 938.480 kb on - strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.633 kb on + strand at 938.634 kb on - strand at 938.634 kb on - strand at 938.673 kb on + strand at 938.673 kb on + strand at 938.673 kb on + strand at 938.674 kb on - strand at 938.693 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1 vol% remove 935,900 + Psyr_0826 0.59 -0.5 935,943 + Psyr_0826 0.61 -1.5 936,039 - Psyr_0826 0.67 -1.5 936,039 - Psyr_0826 0.67 -0.7 936,221 - Psyr_0826 0.78 -2.2 936,333 - Psyr_0826 0.85 +0.8 936,333 - Psyr_0826 0.85 -0.2 936,581 + -0.8 936,582 - -1.8 936,605 + -1.3 936,605 + -1.2 936,687 - +0.5 936,710 + +1.5 936,710 + +0.8 936,710 + -0.2 936,710 + -0.5 936,710 + -0.4 936,710 + -0.2 936,711 - -0.9 936,711 - +0.1 936,711 - +0.5 936,711 - -0.3 936,711 - -0.4 936,711 - +1.8 936,711 - -0.1 936,762 + -0.3 936,817 + -2.3 936,817 + -0.5 936,817 + -2.4 936,817 + +0.3 936,818 - -0.7 936,818 - -0.0 936,818 - +0.2 936,818 - +0.2 936,818 - +0.3 936,818 - +0.5 936,818 - -0.2 936,970 - Psyr_0827 0.15 -2.4 937,080 + Psyr_0827 0.28 -1.5 937,081 - Psyr_0827 0.28 -0.8 937,161 - Psyr_0827 0.37 -1.4 937,398 + Psyr_0827 0.65 -1.4 937,482 + Psyr_0827 0.75 -1.9 937,482 + Psyr_0827 0.75 -2.7 937,482 + Psyr_0827 0.75 -3.5 937,483 - Psyr_0827 0.75 -0.8 937,531 + Psyr_0827 0.81 +0.5 937,683 - -1.1 937,748 + +0.5 937,749 - -1.4 938,311 - Psyr_0828 0.78 -0.4 938,417 + -0.2 938,473 - -0.1 938,479 + -1.2 938,479 + +0.4 938,480 - -1.5 938,480 - +0.9 938,492 + +0.8 938,492 + +0.5 938,492 + -0.4 938,492 + -0.2 938,492 + -0.2 938,492 + -1.8 938,492 + -1.2 938,492 + -0.8 938,492 + -1.2 938,492 + +0.8 938,492 + -0.4 938,492 + -2.0 938,492 + -0.0 938,493 - +0.4 938,493 - -1.9 938,493 - -2.4 938,493 - -0.9 938,493 - +0.5 938,493 - -0.5 938,493 - -0.5 938,493 - +0.0 938,493 - +0.5 938,493 - +0.4 938,493 - +1.4 938,493 - +0.5 938,493 - -0.5 938,633 + -1.4 938,634 - -2.1 938,634 - +0.8 938,673 + -0.5 938,673 + +0.1 938,673 + -0.5 938,674 - -0.5 938,693 + +1.3
Or see this region's nucleotide sequence