Experiment: KB with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0621 and Psyr_0622 are separated by 93 nucleotides Psyr_0622 and Psyr_0623 are separated by 410 nucleotides Psyr_0623 and Psyr_0624 are separated by 353 nucleotides Psyr_0624 and Psyr_0625 are separated by 110 nucleotides
Psyr_0621: Psyr_0621 - conserved hypothetical protein, at 711,095 to 711,946
_0621
Psyr_0622: Psyr_0622 - transcriptional regulator, XRE family, at 712,040 to 712,282
_0622
Psyr_0623: Psyr_0623 - Peptidase S24, S26A and S26B, at 712,693 to 713,424
_0623
Psyr_0624: Psyr_0624 - Inorganic diphosphatase, at 713,778 to 714,305
_0624
Psyr_0625: Psyr_0625 - Protein of unknown function DUF980, at 714,416 to 715,225
_0625
Position (kb) 712
713
714 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 711.693 kb on + strand, within Psyr_0621 at 711.693 kb on + strand, within Psyr_0621 at 711.694 kb on - strand, within Psyr_0621 at 711.694 kb on - strand, within Psyr_0621 at 711.694 kb on - strand, within Psyr_0621 at 711.694 kb on - strand, within Psyr_0621 at 711.738 kb on - strand, within Psyr_0621 at 711.738 kb on - strand, within Psyr_0621 at 711.759 kb on + strand, within Psyr_0621 at 711.760 kb on - strand, within Psyr_0621 at 711.862 kb on + strand at 711.863 kb on - strand at 711.930 kb on + strand at 711.930 kb on + strand at 711.930 kb on + strand at 711.930 kb on + strand at 711.931 kb on - strand at 711.931 kb on - strand at 711.931 kb on - strand at 711.931 kb on - strand at 711.931 kb on - strand at 711.931 kb on - strand at 711.931 kb on - strand at 711.955 kb on + strand at 711.956 kb on - strand at 711.956 kb on - strand at 711.988 kb on - strand at 712.001 kb on + strand at 712.081 kb on - strand, within Psyr_0622 at 712.182 kb on - strand, within Psyr_0622 at 712.193 kb on + strand, within Psyr_0622 at 712.194 kb on - strand, within Psyr_0622 at 712.310 kb on + strand at 712.310 kb on + strand at 712.311 kb on - strand at 712.314 kb on + strand at 712.315 kb on - strand at 712.318 kb on + strand at 712.318 kb on + strand at 712.318 kb on + strand at 712.419 kb on - strand at 712.457 kb on - strand at 712.500 kb on + strand at 712.500 kb on + strand at 712.556 kb on + strand at 712.557 kb on - strand at 712.696 kb on + strand at 712.901 kb on + strand, within Psyr_0623 at 712.901 kb on + strand, within Psyr_0623 at 712.901 kb on + strand, within Psyr_0623 at 712.901 kb on + strand, within Psyr_0623 at 712.901 kb on + strand, within Psyr_0623 at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.418 kb on + strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.419 kb on - strand at 713.458 kb on + strand at 713.458 kb on + strand at 713.458 kb on + strand at 713.458 kb on + strand at 713.458 kb on + strand at 713.515 kb on + strand at 713.537 kb on + strand at 713.537 kb on + strand at 713.538 kb on - strand at 713.538 kb on - strand at 713.538 kb on - strand at 713.538 kb on - strand at 713.538 kb on - strand at 713.553 kb on + strand at 713.577 kb on + strand at 713.577 kb on + strand at 713.578 kb on - strand at 713.578 kb on - strand at 713.578 kb on - strand at 713.631 kb on + strand at 713.710 kb on + strand at 714.356 kb on - strand at 714.373 kb on - strand at 714.373 kb on - strand at 714.379 kb on - strand at 714.379 kb on - strand at 714.379 kb on - strand at 714.379 kb on - strand at 714.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1 vol% remove 711,693 + Psyr_0621 0.70 -0.5 711,693 + Psyr_0621 0.70 +0.7 711,694 - Psyr_0621 0.70 +0.3 711,694 - Psyr_0621 0.70 -0.4 711,694 - Psyr_0621 0.70 -1.1 711,694 - Psyr_0621 0.70 -0.7 711,738 - Psyr_0621 0.75 +0.2 711,738 - Psyr_0621 0.75 -0.7 711,759 + Psyr_0621 0.78 -0.7 711,760 - Psyr_0621 0.78 -0.7 711,862 + +0.1 711,863 - +1.1 711,930 + -0.1 711,930 + -0.5 711,930 + -0.5 711,930 + -0.5 711,931 - +1.7 711,931 - -0.7 711,931 - +0.6 711,931 - +0.4 711,931 - +0.9 711,931 - +0.2 711,931 - -0.0 711,955 + -1.4 711,956 - -0.8 711,956 - +0.1 711,988 - +0.6 712,001 + -2.6 712,081 - Psyr_0622 0.17 +0.2 712,182 - Psyr_0622 0.58 -0.6 712,193 + Psyr_0622 0.63 +0.5 712,194 - Psyr_0622 0.63 +0.9 712,310 + -0.5 712,310 + +1.3 712,311 - -0.7 712,314 + +0.9 712,315 - +0.3 712,318 + -0.8 712,318 + -0.1 712,318 + -0.5 712,419 - -0.6 712,457 - +3.0 712,500 + +0.2 712,500 + +0.6 712,556 + -0.3 712,557 - -0.0 712,696 + -0.5 712,901 + Psyr_0623 0.28 -1.3 712,901 + Psyr_0623 0.28 -0.5 712,901 + Psyr_0623 0.28 +0.1 712,901 + Psyr_0623 0.28 +1.9 712,901 + Psyr_0623 0.28 -0.7 713,418 + +0.7 713,418 + +1.2 713,418 + +0.1 713,418 + +2.2 713,418 + +0.4 713,418 + -1.1 713,418 + +0.1 713,418 + +0.7 713,418 + -1.1 713,418 + -0.3 713,418 + -0.5 713,418 + +0.6 713,418 + -0.4 713,418 + +0.7 713,418 + -0.2 713,418 + +0.9 713,418 + +0.0 713,418 + -1.1 713,418 + +0.2 713,418 + +0.5 713,418 + +0.4 713,419 - -0.2 713,419 - -2.1 713,419 - +1.7 713,419 - +0.1 713,419 - +0.3 713,419 - -0.1 713,419 - -0.6 713,419 - -0.0 713,419 - +0.2 713,419 - +1.3 713,419 - -2.5 713,419 - +0.1 713,419 - -0.3 713,419 - +1.4 713,419 - +0.7 713,419 - -0.4 713,419 - +0.0 713,419 - -1.1 713,419 - -1.7 713,419 - -0.1 713,419 - +0.5 713,419 - +0.2 713,419 - +0.0 713,419 - -0.5 713,419 - +0.9 713,458 + +0.2 713,458 + -0.9 713,458 + -0.4 713,458 + -0.5 713,458 + +0.2 713,515 + +0.2 713,537 + +0.7 713,537 + +0.9 713,538 - -0.1 713,538 - -0.7 713,538 - -0.1 713,538 - -1.1 713,538 - -0.8 713,553 + +0.5 713,577 + -1.6 713,577 + +0.1 713,578 - +0.1 713,578 - -1.3 713,578 - -0.9 713,631 + +1.0 713,710 + +3.2 714,356 - -1.2 714,373 - -0.7 714,373 - -0.8 714,379 - -0.7 714,379 - +0.9 714,379 - +1.1 714,379 - -0.9 714,379 - +0.4
Or see this region's nucleotide sequence