Experiment: KB with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0404 and Psyr_0405 overlap by 4 nucleotides Psyr_0405 and Psyr_0406 overlap by 4 nucleotides Psyr_0406 and Psyr_0407 overlap by 4 nucleotides
Psyr_0404: Psyr_0404 - Fimbrial assembly, at 434,688 to 435,263
_0404
Psyr_0405: Psyr_0405 - Pilus assembly protein, PilO, at 435,260 to 435,883
_0405
Psyr_0406: Psyr_0406 - Pilus assembly protein, PilQ, at 435,880 to 436,407
_0406
Psyr_0407: Psyr_0407 - type II and III secretion system protein:NolW-like protein:NolW-like protein, at 436,404 to 438,560
_0407
Position (kb) 435
436
437 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 435.027 kb on + strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.028 kb on - strand, within Psyr_0404 at 435.030 kb on + strand, within Psyr_0404 at 435.214 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.265 kb on + strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.266 kb on - strand at 435.398 kb on + strand, within Psyr_0405 at 435.398 kb on + strand, within Psyr_0405 at 435.398 kb on + strand, within Psyr_0405 at 435.399 kb on - strand, within Psyr_0405 at 435.512 kb on + strand, within Psyr_0405 at 435.513 kb on - strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.852 kb on - strand at 435.858 kb on - strand at 435.858 kb on - strand at 436.048 kb on + strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.276 kb on + strand, within Psyr_0406 at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.489 kb on + strand at 436.618 kb on - strand at 436.665 kb on - strand, within Psyr_0407 at 436.729 kb on + strand, within Psyr_0407 at 437.202 kb on + strand, within Psyr_0407 at 437.295 kb on + strand, within Psyr_0407 at 437.295 kb on + strand, within Psyr_0407 at 437.296 kb on - strand, within Psyr_0407 at 437.296 kb on - strand, within Psyr_0407 at 437.397 kb on - strand, within Psyr_0407
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1 vol% remove 435,027 + Psyr_0404 0.59 +0.0 435,028 - Psyr_0404 0.59 +0.3 435,028 - Psyr_0404 0.59 -1.2 435,028 - Psyr_0404 0.59 +0.7 435,030 + Psyr_0404 0.59 -1.8 435,214 + -0.5 435,265 + +0.9 435,265 + +1.1 435,265 + -1.3 435,265 + +1.3 435,265 + -0.4 435,265 + -0.6 435,265 + -0.3 435,265 + -0.3 435,265 + +0.4 435,265 + +1.4 435,265 + -0.6 435,265 + +1.4 435,265 + -0.6 435,265 + -0.6 435,265 + +0.2 435,265 + +3.3 435,265 + +1.2 435,266 - +0.3 435,266 - +0.2 435,266 - -0.3 435,266 - -1.6 435,266 - +0.5 435,266 - -0.3 435,266 - +0.2 435,266 - -0.6 435,398 + Psyr_0405 0.22 +0.7 435,398 + Psyr_0405 0.22 -0.7 435,398 + Psyr_0405 0.22 -0.3 435,399 - Psyr_0405 0.22 -2.5 435,512 + Psyr_0405 0.40 +0.0 435,513 - Psyr_0405 0.41 -0.4 435,680 + Psyr_0405 0.67 -1.5 435,680 + Psyr_0405 0.67 -0.3 435,680 + Psyr_0405 0.67 -1.0 435,680 + Psyr_0405 0.67 +0.0 435,681 - Psyr_0405 0.67 -0.6 435,852 - -0.2 435,858 - -0.2 435,858 - +0.4 436,048 + Psyr_0406 0.32 -1.0 436,048 + Psyr_0406 0.32 +0.4 436,048 + Psyr_0406 0.32 -1.0 436,048 + Psyr_0406 0.32 +0.0 436,049 - Psyr_0406 0.32 -0.4 436,049 - Psyr_0406 0.32 +1.7 436,049 - Psyr_0406 0.32 -0.4 436,276 + Psyr_0406 0.75 -0.7 436,424 + -1.2 436,424 + -0.7 436,424 + +0.1 436,424 + +1.4 436,424 + -0.4 436,424 + +2.6 436,424 + +0.8 436,424 + +0.5 436,424 + -0.4 436,424 + +0.4 436,424 + +1.0 436,424 + -1.4 436,424 + -0.1 436,424 + +0.3 436,424 + -0.6 436,425 - +0.1 436,425 - +0.9 436,425 - -0.8 436,425 - +0.2 436,425 - -1.3 436,425 - -0.6 436,425 - -0.3 436,425 - -0.6 436,425 - +0.0 436,425 - +1.0 436,489 + -0.8 436,618 - +1.0 436,665 - Psyr_0407 0.12 -1.8 436,729 + Psyr_0407 0.15 +0.3 437,202 + Psyr_0407 0.37 +0.5 437,295 + Psyr_0407 0.41 +0.2 437,295 + Psyr_0407 0.41 +0.4 437,296 - Psyr_0407 0.41 +1.0 437,296 - Psyr_0407 0.41 +2.6 437,397 - Psyr_0407 0.46 +0.2
Or see this region's nucleotide sequence