Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4203 and Pf6N2E2_4204 are separated by 5 nucleotides Pf6N2E2_4204 and Pf6N2E2_4205 are separated by 18 nucleotides Pf6N2E2_4205 and Pf6N2E2_4206 are separated by 9 nucleotides
Pf6N2E2_4203 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 4,894,028 to 4,895,926
_4203
Pf6N2E2_4204 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 4,895,932 to 4,896,567
_4204
Pf6N2E2_4205 - Chromosome (plasmid) partitioning protein ParA, at 4,896,586 to 4,897,383
_4205
Pf6N2E2_4206 - Chromosome (plasmid) partitioning protein ParB, at 4,897,393 to 4,898,265
_4206
Position (kb)
4896
4897
4898 Strain fitness (log2 ratio)
-2
-1
0
1 at 4896.059 kb on - strand, within Pf6N2E2_4204 at 4896.059 kb on - strand, within Pf6N2E2_4204 at 4896.304 kb on - strand, within Pf6N2E2_4204 at 4896.549 kb on - strand at 4896.592 kb on - strand at 4896.597 kb on - strand at 4896.597 kb on - strand at 4896.646 kb on - strand at 4896.766 kb on + strand, within Pf6N2E2_4205 at 4896.767 kb on - strand, within Pf6N2E2_4205 at 4896.941 kb on - strand, within Pf6N2E2_4205 at 4896.947 kb on - strand, within Pf6N2E2_4205 at 4896.947 kb on - strand, within Pf6N2E2_4205 at 4896.947 kb on - strand, within Pf6N2E2_4205 at 4897.039 kb on + strand, within Pf6N2E2_4205 at 4897.040 kb on - strand, within Pf6N2E2_4205 at 4897.040 kb on - strand, within Pf6N2E2_4205 at 4897.142 kb on - strand, within Pf6N2E2_4205 at 4897.142 kb on - strand, within Pf6N2E2_4205 at 4897.381 kb on + strand at 4897.381 kb on + strand at 4897.537 kb on + strand, within Pf6N2E2_4206 at 4897.537 kb on + strand, within Pf6N2E2_4206 at 4897.538 kb on - strand, within Pf6N2E2_4206 at 4898.280 kb on + strand at 4898.280 kb on + strand at 4898.280 kb on + strand at 4898.301 kb on + strand at 4898.301 kb on + strand at 4898.301 kb on + strand at 4898.301 kb on + strand at 4898.334 kb on + strand at 4898.336 kb on + strand at 4898.365 kb on + strand at 4898.383 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 4,896,059 - Pf6N2E2_4204 0.20 +0.2 4,896,059 - Pf6N2E2_4204 0.20 -0.6 4,896,304 - Pf6N2E2_4204 0.58 -1.1 4,896,549 - +0.2 4,896,592 - -0.4 4,896,597 - -0.2 4,896,597 - -0.1 4,896,646 - +0.8 4,896,766 + Pf6N2E2_4205 0.23 -0.1 4,896,767 - Pf6N2E2_4205 0.23 -0.8 4,896,941 - Pf6N2E2_4205 0.44 -0.5 4,896,947 - Pf6N2E2_4205 0.45 -0.5 4,896,947 - Pf6N2E2_4205 0.45 -0.8 4,896,947 - Pf6N2E2_4205 0.45 +1.4 4,897,039 + Pf6N2E2_4205 0.57 -1.1 4,897,040 - Pf6N2E2_4205 0.57 -0.2 4,897,040 - Pf6N2E2_4205 0.57 -1.1 4,897,142 - Pf6N2E2_4205 0.70 -0.8 4,897,142 - Pf6N2E2_4205 0.70 +0.8 4,897,381 + -1.4 4,897,381 + -1.8 4,897,537 + Pf6N2E2_4206 0.16 +1.0 4,897,537 + Pf6N2E2_4206 0.16 +0.3 4,897,538 - Pf6N2E2_4206 0.17 -0.9 4,898,280 + -1.1 4,898,280 + +0.5 4,898,280 + +0.3 4,898,301 + -0.8 4,898,301 + +0.3 4,898,301 + +0.2 4,898,301 + -0.3 4,898,334 + +0.3 4,898,336 + -0.9 4,898,365 + +1.2 4,898,383 + +0.4
Or see this region's nucleotide sequence