Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1707 and Pf6N2E2_1708 overlap by 42 nucleotides Pf6N2E2_1708 and Pf6N2E2_1709 are separated by 291 nucleotides Pf6N2E2_1709 and Pf6N2E2_1710 are separated by 43 nucleotides
Pf6N2E2_1707 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 2,015,530 to 2,016,915
_1707
Pf6N2E2_1708 - hypothetical protein, at 2,016,874 to 2,017,026
_1708
Pf6N2E2_1709 - multidrug resistance transporter, Bcr/CflA family, at 2,017,318 to 2,018,538
_1709
Pf6N2E2_1710 - FIG00953587: hypothetical protein, at 2,018,582 to 2,019,856
_1710
Position (kb)
2017
2018
2019 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2016.443 kb on + strand, within Pf6N2E2_1707 at 2016.444 kb on - strand, within Pf6N2E2_1707 at 2016.459 kb on - strand, within Pf6N2E2_1707 at 2016.536 kb on + strand, within Pf6N2E2_1707 at 2016.536 kb on + strand, within Pf6N2E2_1707 at 2016.536 kb on + strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.766 kb on - strand, within Pf6N2E2_1707 at 2016.852 kb on - strand at 2017.127 kb on + strand at 2017.128 kb on - strand at 2017.128 kb on - strand at 2017.128 kb on - strand at 2017.128 kb on - strand at 2017.138 kb on + strand at 2017.139 kb on - strand at 2017.139 kb on - strand at 2017.139 kb on - strand at 2017.195 kb on + strand at 2017.300 kb on + strand at 2017.301 kb on - strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.318 kb on - strand at 2017.318 kb on - strand at 2017.318 kb on - strand at 2017.318 kb on - strand at 2017.904 kb on + strand, within Pf6N2E2_1709 at 2017.904 kb on + strand, within Pf6N2E2_1709 at 2017.976 kb on - strand, within Pf6N2E2_1709 at 2018.120 kb on - strand, within Pf6N2E2_1709 at 2018.120 kb on - strand, within Pf6N2E2_1709 at 2018.281 kb on + strand, within Pf6N2E2_1709 at 2018.281 kb on + strand, within Pf6N2E2_1709 at 2018.675 kb on + strand at 2018.676 kb on - strand at 2018.752 kb on - strand, within Pf6N2E2_1710 at 2019.029 kb on + strand, within Pf6N2E2_1710 at 2019.029 kb on + strand, within Pf6N2E2_1710 at 2019.029 kb on + strand, within Pf6N2E2_1710 at 2019.029 kb on + strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.031 kb on + strand, within Pf6N2E2_1710 at 2019.105 kb on + strand, within Pf6N2E2_1710 at 2019.106 kb on - strand, within Pf6N2E2_1710 at 2019.123 kb on + strand, within Pf6N2E2_1710 at 2019.363 kb on + strand, within Pf6N2E2_1710 at 2019.364 kb on - strand, within Pf6N2E2_1710 at 2019.364 kb on - strand, within Pf6N2E2_1710 at 2019.442 kb on - strand, within Pf6N2E2_1710
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,016,443 + Pf6N2E2_1707 0.66 -0.0 2,016,444 - Pf6N2E2_1707 0.66 -0.2 2,016,459 - Pf6N2E2_1707 0.67 -0.2 2,016,536 + Pf6N2E2_1707 0.73 +0.7 2,016,536 + Pf6N2E2_1707 0.73 -0.3 2,016,536 + Pf6N2E2_1707 0.73 -0.8 2,016,762 - Pf6N2E2_1707 0.89 +1.1 2,016,762 - Pf6N2E2_1707 0.89 -1.3 2,016,762 - Pf6N2E2_1707 0.89 +0.3 2,016,762 - Pf6N2E2_1707 0.89 +2.1 2,016,762 - Pf6N2E2_1707 0.89 -1.7 2,016,762 - Pf6N2E2_1707 0.89 +0.6 2,016,762 - Pf6N2E2_1707 0.89 +1.1 2,016,766 - Pf6N2E2_1707 0.89 +0.2 2,016,852 - -0.6 2,017,127 + -0.2 2,017,128 - -0.2 2,017,128 - -0.1 2,017,128 - +2.1 2,017,128 - +1.6 2,017,138 + +0.9 2,017,139 - +0.1 2,017,139 - +0.9 2,017,139 - +0.0 2,017,195 + -0.0 2,017,300 + +0.3 2,017,301 - +0.6 2,017,317 + +0.7 2,017,317 + +0.7 2,017,317 + -0.7 2,017,317 + +0.1 2,017,317 + -1.1 2,017,317 + -0.2 2,017,317 + +0.4 2,017,317 + -1.0 2,017,318 - -0.2 2,017,318 - -1.0 2,017,318 - +2.1 2,017,318 - -1.4 2,017,904 + Pf6N2E2_1709 0.48 +0.8 2,017,904 + Pf6N2E2_1709 0.48 -2.7 2,017,976 - Pf6N2E2_1709 0.54 -0.7 2,018,120 - Pf6N2E2_1709 0.66 -1.2 2,018,120 - Pf6N2E2_1709 0.66 -1.4 2,018,281 + Pf6N2E2_1709 0.79 -0.9 2,018,281 + Pf6N2E2_1709 0.79 +0.6 2,018,675 + +1.1 2,018,676 - +0.1 2,018,752 - Pf6N2E2_1710 0.13 +0.0 2,019,029 + Pf6N2E2_1710 0.35 -0.9 2,019,029 + Pf6N2E2_1710 0.35 -0.8 2,019,029 + Pf6N2E2_1710 0.35 +1.2 2,019,029 + Pf6N2E2_1710 0.35 +0.6 2,019,030 - Pf6N2E2_1710 0.35 -0.4 2,019,030 - Pf6N2E2_1710 0.35 +0.4 2,019,030 - Pf6N2E2_1710 0.35 -0.5 2,019,030 - Pf6N2E2_1710 0.35 +0.6 2,019,030 - Pf6N2E2_1710 0.35 -1.4 2,019,030 - Pf6N2E2_1710 0.35 -2.1 2,019,030 - Pf6N2E2_1710 0.35 -1.0 2,019,030 - Pf6N2E2_1710 0.35 +1.8 2,019,031 + Pf6N2E2_1710 0.35 +0.6 2,019,105 + Pf6N2E2_1710 0.41 -0.2 2,019,106 - Pf6N2E2_1710 0.41 -0.8 2,019,123 + Pf6N2E2_1710 0.42 +0.1 2,019,363 + Pf6N2E2_1710 0.61 -1.0 2,019,364 - Pf6N2E2_1710 0.61 +0.3 2,019,364 - Pf6N2E2_1710 0.61 +0.8 2,019,442 - Pf6N2E2_1710 0.67 +0.5
Or see this region's nucleotide sequence