Experiment: R2A with sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08135 and CA265_RS08140 are separated by 43 nucleotides CA265_RS08140 and CA265_RS08145 are separated by 280 nucleotides
CA265_RS08135: CA265_RS08135 - amidohydrolase, at 2,018,112 to 2,018,891
_RS08135
CA265_RS08140: CA265_RS08140 - methionine aminotransferase, at 2,018,935 to 2,020,080
_RS08140
CA265_RS08145: CA265_RS08145 - hypothetical protein, at 2,020,361 to 2,021,893
_RS08145
Position (kb)
2018
2019
2020
2021 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2017.955 kb on + strand at 2017.996 kb on + strand at 2018.014 kb on + strand at 2018.030 kb on + strand at 2018.054 kb on + strand at 2018.055 kb on - strand at 2018.163 kb on + strand at 2018.224 kb on + strand, within CA265_RS08135 at 2018.227 kb on + strand, within CA265_RS08135 at 2018.227 kb on + strand, within CA265_RS08135 at 2018.321 kb on - strand, within CA265_RS08135 at 2018.321 kb on - strand, within CA265_RS08135 at 2018.451 kb on + strand, within CA265_RS08135 at 2018.502 kb on - strand, within CA265_RS08135 at 2018.721 kb on + strand, within CA265_RS08135 at 2018.775 kb on - strand, within CA265_RS08135 at 2018.775 kb on - strand, within CA265_RS08135 at 2018.810 kb on + strand, within CA265_RS08135 at 2018.810 kb on + strand, within CA265_RS08135 at 2018.810 kb on + strand, within CA265_RS08135 at 2018.811 kb on - strand, within CA265_RS08135 at 2018.874 kb on + strand at 2018.899 kb on + strand at 2018.900 kb on - strand at 2019.108 kb on + strand, within CA265_RS08140 at 2019.119 kb on + strand, within CA265_RS08140 at 2019.119 kb on + strand, within CA265_RS08140 at 2019.120 kb on - strand, within CA265_RS08140 at 2019.120 kb on - strand, within CA265_RS08140 at 2019.125 kb on - strand, within CA265_RS08140 at 2019.265 kb on - strand, within CA265_RS08140 at 2019.283 kb on - strand, within CA265_RS08140 at 2019.372 kb on + strand, within CA265_RS08140 at 2019.400 kb on + strand, within CA265_RS08140 at 2019.401 kb on - strand, within CA265_RS08140 at 2019.403 kb on - strand, within CA265_RS08140 at 2019.403 kb on - strand, within CA265_RS08140 at 2019.439 kb on + strand, within CA265_RS08140 at 2019.440 kb on - strand, within CA265_RS08140 at 2019.510 kb on + strand, within CA265_RS08140 at 2019.525 kb on + strand, within CA265_RS08140 at 2019.586 kb on + strand, within CA265_RS08140 at 2019.591 kb on + strand, within CA265_RS08140 at 2019.592 kb on - strand, within CA265_RS08140 at 2019.630 kb on + strand, within CA265_RS08140 at 2019.642 kb on + strand at 2019.643 kb on - strand, within CA265_RS08140 at 2019.648 kb on - strand, within CA265_RS08140 at 2019.648 kb on - strand, within CA265_RS08140 at 2019.648 kb on - strand, within CA265_RS08140 at 2019.720 kb on - strand, within CA265_RS08140 at 2019.738 kb on + strand, within CA265_RS08140 at 2019.758 kb on - strand, within CA265_RS08140 at 2019.759 kb on + strand, within CA265_RS08140 at 2019.760 kb on - strand, within CA265_RS08140 at 2019.843 kb on - strand, within CA265_RS08140 at 2019.933 kb on + strand, within CA265_RS08140 at 2019.994 kb on - strand at 2020.045 kb on - strand at 2020.049 kb on - strand at 2020.069 kb on + strand at 2020.069 kb on + strand at 2020.075 kb on - strand at 2020.083 kb on + strand at 2020.086 kb on - strand at 2020.110 kb on + strand at 2020.130 kb on + strand at 2020.131 kb on - strand at 2020.131 kb on - strand at 2020.232 kb on + strand at 2020.277 kb on - strand at 2020.288 kb on - strand at 2020.297 kb on + strand at 2020.356 kb on + strand at 2020.361 kb on - strand at 2020.401 kb on + strand at 2020.482 kb on + strand at 2020.482 kb on + strand at 2020.483 kb on - strand at 2020.483 kb on - strand at 2020.542 kb on + strand, within CA265_RS08145 at 2020.543 kb on - strand, within CA265_RS08145 at 2020.552 kb on - strand, within CA265_RS08145 at 2020.662 kb on + strand, within CA265_RS08145 at 2020.680 kb on - strand, within CA265_RS08145 at 2020.717 kb on + strand, within CA265_RS08145 at 2020.723 kb on + strand, within CA265_RS08145 at 2020.817 kb on + strand, within CA265_RS08145 at 2020.818 kb on - strand, within CA265_RS08145 at 2020.838 kb on - strand, within CA265_RS08145 at 2020.869 kb on + strand, within CA265_RS08145 at 2020.875 kb on + strand, within CA265_RS08145 at 2020.897 kb on + strand, within CA265_RS08145 at 2020.897 kb on + strand, within CA265_RS08145 at 2020.898 kb on - strand, within CA265_RS08145 at 2020.906 kb on + strand, within CA265_RS08145 at 2020.907 kb on - strand, within CA265_RS08145 at 2020.907 kb on - strand, within CA265_RS08145 at 2020.950 kb on + strand, within CA265_RS08145 at 2020.950 kb on + strand, within CA265_RS08145 at 2020.950 kb on + strand, within CA265_RS08145 at 2020.961 kb on - strand, within CA265_RS08145 at 2020.978 kb on - strand, within CA265_RS08145 at 2020.982 kb on - strand, within CA265_RS08145 at 2021.003 kb on - strand, within CA265_RS08145 at 2021.005 kb on - strand, within CA265_RS08145
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 12.5 mM remove 2,017,955 + -0.9 2,017,996 + -0.1 2,018,014 + +0.5 2,018,030 + -0.4 2,018,054 + -0.7 2,018,055 - +0.1 2,018,163 + -0.1 2,018,224 + CA265_RS08135 0.14 -0.0 2,018,227 + CA265_RS08135 0.15 -0.6 2,018,227 + CA265_RS08135 0.15 +0.3 2,018,321 - CA265_RS08135 0.27 +2.0 2,018,321 - CA265_RS08135 0.27 -0.6 2,018,451 + CA265_RS08135 0.43 +1.2 2,018,502 - CA265_RS08135 0.50 -2.0 2,018,721 + CA265_RS08135 0.78 -0.4 2,018,775 - CA265_RS08135 0.85 +0.1 2,018,775 - CA265_RS08135 0.85 -0.3 2,018,810 + CA265_RS08135 0.89 +0.3 2,018,810 + CA265_RS08135 0.89 +0.0 2,018,810 + CA265_RS08135 0.89 +0.2 2,018,811 - CA265_RS08135 0.90 -0.1 2,018,874 + -0.2 2,018,899 + +0.8 2,018,900 - +1.2 2,019,108 + CA265_RS08140 0.15 +0.7 2,019,119 + CA265_RS08140 0.16 -0.1 2,019,119 + CA265_RS08140 0.16 -0.5 2,019,120 - CA265_RS08140 0.16 +0.7 2,019,120 - CA265_RS08140 0.16 +0.0 2,019,125 - CA265_RS08140 0.17 -0.2 2,019,265 - CA265_RS08140 0.29 -1.0 2,019,283 - CA265_RS08140 0.30 +0.5 2,019,372 + CA265_RS08140 0.38 +0.3 2,019,400 + CA265_RS08140 0.41 +0.3 2,019,401 - CA265_RS08140 0.41 -0.2 2,019,403 - CA265_RS08140 0.41 +0.1 2,019,403 - CA265_RS08140 0.41 +0.6 2,019,439 + CA265_RS08140 0.44 +0.1 2,019,440 - CA265_RS08140 0.44 +0.1 2,019,510 + CA265_RS08140 0.50 -0.2 2,019,525 + CA265_RS08140 0.51 +0.6 2,019,586 + CA265_RS08140 0.57 +0.1 2,019,591 + CA265_RS08140 0.57 -0.3 2,019,592 - CA265_RS08140 0.57 -0.0 2,019,630 + CA265_RS08140 0.61 +0.2 2,019,642 + +0.6 2,019,643 - CA265_RS08140 0.62 +0.8 2,019,648 - CA265_RS08140 0.62 -0.1 2,019,648 - CA265_RS08140 0.62 +0.4 2,019,648 - CA265_RS08140 0.62 -0.9 2,019,720 - CA265_RS08140 0.68 +0.6 2,019,738 + CA265_RS08140 0.70 -0.3 2,019,758 - CA265_RS08140 0.72 +0.5 2,019,759 + CA265_RS08140 0.72 +0.3 2,019,760 - CA265_RS08140 0.72 +0.5 2,019,843 - CA265_RS08140 0.79 +0.3 2,019,933 + CA265_RS08140 0.87 +0.1 2,019,994 - -0.0 2,020,045 - +0.2 2,020,049 - -1.6 2,020,069 + -0.7 2,020,069 + -1.5 2,020,075 - -0.3 2,020,083 + +0.8 2,020,086 - +1.3 2,020,110 + -1.1 2,020,130 + +1.2 2,020,131 - -0.6 2,020,131 - +0.6 2,020,232 + +0.5 2,020,277 - -1.2 2,020,288 - +0.4 2,020,297 + -0.6 2,020,356 + -1.0 2,020,361 - -1.2 2,020,401 + +0.5 2,020,482 + +0.3 2,020,482 + -0.2 2,020,483 - -0.1 2,020,483 - +0.4 2,020,542 + CA265_RS08145 0.12 -0.4 2,020,543 - CA265_RS08145 0.12 +0.3 2,020,552 - CA265_RS08145 0.12 -0.3 2,020,662 + CA265_RS08145 0.20 -0.5 2,020,680 - CA265_RS08145 0.21 -0.4 2,020,717 + CA265_RS08145 0.23 -0.3 2,020,723 + CA265_RS08145 0.24 +0.0 2,020,817 + CA265_RS08145 0.30 -0.4 2,020,818 - CA265_RS08145 0.30 +1.6 2,020,838 - CA265_RS08145 0.31 +0.1 2,020,869 + CA265_RS08145 0.33 +0.3 2,020,875 + CA265_RS08145 0.34 -0.2 2,020,897 + CA265_RS08145 0.35 -0.3 2,020,897 + CA265_RS08145 0.35 +0.6 2,020,898 - CA265_RS08145 0.35 -0.3 2,020,906 + CA265_RS08145 0.36 +0.7 2,020,907 - CA265_RS08145 0.36 -0.3 2,020,907 - CA265_RS08145 0.36 -0.3 2,020,950 + CA265_RS08145 0.38 +0.4 2,020,950 + CA265_RS08145 0.38 -1.8 2,020,950 + CA265_RS08145 0.38 +0.7 2,020,961 - CA265_RS08145 0.39 +0.7 2,020,978 - CA265_RS08145 0.40 -0.7 2,020,982 - CA265_RS08145 0.41 +0.0 2,021,003 - CA265_RS08145 0.42 -0.3 2,021,005 - CA265_RS08145 0.42 -0.3
Or see this region's nucleotide sequence