Experiment: R2A with Thallium(I) acetate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35425 and RR42_RS35430 are separated by 585 nucleotides RR42_RS35430 and RR42_RS35435 are separated by 680 nucleotides
RR42_RS35425: RR42_RS35425 - aspartate carbamoyltransferase, at 3,400,420 to 3,400,941
_RS35425
RR42_RS35430: RR42_RS35430 - hypothetical protein, at 3,401,527 to 3,401,907
_RS35430
RR42_RS35435: RR42_RS35435 - NAD(FAD)-dependent dehydrogenase, at 3,402,588 to 3,404,255
_RS35435
Position (kb)
3401
3402 Strain fitness (log2 ratio)
-2
-1
0
1 at 3400.543 kb on - strand, within RR42_RS35425 at 3400.601 kb on + strand, within RR42_RS35425 at 3400.777 kb on + strand, within RR42_RS35425 at 3400.778 kb on - strand, within RR42_RS35425 at 3400.810 kb on + strand, within RR42_RS35425 at 3400.810 kb on + strand, within RR42_RS35425 at 3400.929 kb on + strand at 3401.026 kb on + strand at 3401.026 kb on + strand at 3401.065 kb on - strand at 3401.120 kb on + strand at 3401.225 kb on + strand at 3401.225 kb on + strand at 3401.225 kb on + strand at 3401.226 kb on - strand at 3401.234 kb on + strand at 3401.234 kb on + strand at 3401.235 kb on - strand at 3401.235 kb on - strand at 3401.270 kb on - strand at 3401.279 kb on - strand at 3401.486 kb on + strand at 3401.487 kb on - strand at 3401.534 kb on + strand at 3401.534 kb on + strand at 3401.535 kb on - strand at 3401.535 kb on - strand at 3401.545 kb on + strand at 3401.594 kb on - strand, within RR42_RS35430 at 3401.594 kb on - strand, within RR42_RS35430 at 3401.622 kb on + strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.625 kb on + strand, within RR42_RS35430 at 3401.625 kb on + strand, within RR42_RS35430 at 3401.626 kb on - strand, within RR42_RS35430 at 3401.714 kb on + strand, within RR42_RS35430 at 3401.714 kb on + strand, within RR42_RS35430 at 3401.718 kb on + strand, within RR42_RS35430 at 3401.719 kb on - strand, within RR42_RS35430 at 3401.719 kb on - strand, within RR42_RS35430 at 3401.764 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.831 kb on - strand, within RR42_RS35430 at 3401.859 kb on - strand, within RR42_RS35430 at 3401.859 kb on - strand, within RR42_RS35430 at 3401.871 kb on + strand at 3401.872 kb on - strand at 3402.155 kb on - strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.222 kb on - strand at 3402.222 kb on - strand at 3402.222 kb on - strand at 3402.225 kb on + strand at 3402.226 kb on - strand at 3402.226 kb on - strand at 3402.326 kb on + strand at 3402.326 kb on + strand at 3402.327 kb on - strand at 3402.349 kb on - strand at 3402.381 kb on + strand at 3402.382 kb on - strand at 3402.382 kb on - strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.445 kb on + strand at 3402.445 kb on + strand at 3402.445 kb on + strand at 3402.446 kb on - strand at 3402.446 kb on - strand at 3402.446 kb on - strand at 3402.449 kb on + strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.564 kb on + strand at 3402.564 kb on + strand at 3402.565 kb on - strand at 3402.577 kb on + strand at 3402.578 kb on - strand at 3402.590 kb on - strand at 3402.729 kb on - strand at 3402.729 kb on - strand at 3402.748 kb on + strand at 3402.815 kb on - strand, within RR42_RS35435 at 3402.835 kb on + strand, within RR42_RS35435
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.001 mg/ml remove 3,400,543 - RR42_RS35425 0.24 -0.5 3,400,601 + RR42_RS35425 0.35 -0.4 3,400,777 + RR42_RS35425 0.68 +0.5 3,400,778 - RR42_RS35425 0.69 -0.4 3,400,810 + RR42_RS35425 0.75 -1.4 3,400,810 + RR42_RS35425 0.75 -0.4 3,400,929 + -0.9 3,401,026 + +0.7 3,401,026 + +0.4 3,401,065 - +0.5 3,401,120 + -0.4 3,401,225 + +0.3 3,401,225 + -0.7 3,401,225 + -0.9 3,401,226 - +0.1 3,401,234 + -1.0 3,401,234 + +0.5 3,401,235 - -0.3 3,401,235 - +0.4 3,401,270 - -0.8 3,401,279 - +0.9 3,401,486 + -0.6 3,401,487 - +0.2 3,401,534 + -0.4 3,401,534 + +1.1 3,401,535 - +0.0 3,401,535 - -0.0 3,401,545 + +0.0 3,401,594 - RR42_RS35430 0.18 -2.0 3,401,594 - RR42_RS35430 0.18 -0.5 3,401,622 + RR42_RS35430 0.25 +0.2 3,401,623 - RR42_RS35430 0.25 +0.0 3,401,623 - RR42_RS35430 0.25 +0.5 3,401,623 - RR42_RS35430 0.25 -1.4 3,401,625 + RR42_RS35430 0.26 -1.5 3,401,625 + RR42_RS35430 0.26 -0.7 3,401,626 - RR42_RS35430 0.26 +1.3 3,401,714 + RR42_RS35430 0.49 -1.3 3,401,714 + RR42_RS35430 0.49 +1.2 3,401,718 + RR42_RS35430 0.50 +1.7 3,401,719 - RR42_RS35430 0.50 +0.1 3,401,719 - RR42_RS35430 0.50 +0.1 3,401,764 + RR42_RS35430 0.62 +0.1 3,401,780 + RR42_RS35430 0.66 +0.8 3,401,780 + RR42_RS35430 0.66 +0.4 3,401,780 + RR42_RS35430 0.66 +0.2 3,401,780 + RR42_RS35430 0.66 +0.6 3,401,781 - RR42_RS35430 0.67 +0.7 3,401,781 - RR42_RS35430 0.67 +0.0 3,401,781 - RR42_RS35430 0.67 -0.7 3,401,831 - RR42_RS35430 0.80 +0.9 3,401,859 - RR42_RS35430 0.87 +1.3 3,401,859 - RR42_RS35430 0.87 -0.2 3,401,871 + +1.0 3,401,872 - -0.7 3,402,155 - -0.6 3,402,221 + +1.4 3,402,221 + -1.7 3,402,221 + -0.2 3,402,221 + -0.0 3,402,221 + -0.7 3,402,222 - +0.0 3,402,222 - -0.4 3,402,222 - +0.3 3,402,225 + +0.9 3,402,226 - +0.1 3,402,226 - -0.4 3,402,326 + -0.7 3,402,326 + +0.5 3,402,327 - +1.2 3,402,349 - +1.7 3,402,381 + -2.4 3,402,382 - +1.0 3,402,382 - -0.7 3,402,443 + +0.1 3,402,443 + +0.0 3,402,443 + -1.2 3,402,443 + +0.1 3,402,445 + +0.5 3,402,445 + -1.4 3,402,445 + -1.0 3,402,446 - +0.5 3,402,446 - +1.7 3,402,446 - +0.0 3,402,449 + -1.9 3,402,450 - -0.0 3,402,450 - -0.6 3,402,450 - +1.3 3,402,450 - +0.4 3,402,450 - -0.1 3,402,450 - -0.7 3,402,564 + -0.9 3,402,564 + -0.7 3,402,565 - +0.2 3,402,577 + -0.1 3,402,578 - +0.6 3,402,590 - -0.8 3,402,729 - +0.2 3,402,729 - -0.1 3,402,748 + +0.6 3,402,815 - RR42_RS35435 0.14 -0.9 3,402,835 + RR42_RS35435 0.15 +0.7
Or see this region's nucleotide sequence