Experiment: R2A with Thallium(I) acetate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25185 and RR42_RS25190 are separated by 171 nucleotides RR42_RS25190 and RR42_RS25195 are separated by 66 nucleotides
RR42_RS25185: RR42_RS25185 - epimerase, at 990,375 to 991,253
_RS25185
RR42_RS25190: RR42_RS25190 - acyl-CoA dehydrogenase, at 991,425 to 993,194
_RS25190
RR42_RS25195: RR42_RS25195 - AMP-dependent synthetase, at 993,261 to 994,934
_RS25195
Position (kb)
991
992
993
994 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 990.584 kb on - strand, within RR42_RS25185 at 990.587 kb on + strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.715 kb on - strand, within RR42_RS25185 at 990.845 kb on + strand, within RR42_RS25185 at 990.845 kb on + strand, within RR42_RS25185 at 990.846 kb on - strand, within RR42_RS25185 at 991.184 kb on + strand at 991.239 kb on - strand at 991.260 kb on + strand at 991.261 kb on - strand at 991.273 kb on + strand at 991.273 kb on + strand at 991.273 kb on + strand at 991.274 kb on - strand at 991.274 kb on - strand at 991.359 kb on + strand at 991.360 kb on - strand at 991.489 kb on - strand at 991.489 kb on - strand at 991.513 kb on + strand at 991.513 kb on + strand at 991.543 kb on + strand at 991.544 kb on - strand at 991.544 kb on - strand at 991.544 kb on - strand at 991.609 kb on - strand, within RR42_RS25190 at 991.609 kb on - strand, within RR42_RS25190 at 991.636 kb on + strand, within RR42_RS25190 at 991.636 kb on + strand, within RR42_RS25190 at 991.636 kb on + strand, within RR42_RS25190 at 991.637 kb on - strand, within RR42_RS25190 at 991.637 kb on - strand, within RR42_RS25190 at 991.689 kb on + strand, within RR42_RS25190 at 991.689 kb on + strand, within RR42_RS25190 at 991.689 kb on + strand, within RR42_RS25190 at 991.899 kb on - strand, within RR42_RS25190 at 991.932 kb on - strand, within RR42_RS25190 at 991.941 kb on - strand, within RR42_RS25190 at 991.941 kb on - strand, within RR42_RS25190 at 991.942 kb on + strand, within RR42_RS25190 at 991.943 kb on - strand, within RR42_RS25190 at 991.943 kb on - strand, within RR42_RS25190 at 991.943 kb on - strand, within RR42_RS25190 at 991.943 kb on - strand, within RR42_RS25190 at 992.012 kb on + strand, within RR42_RS25190 at 992.137 kb on + strand, within RR42_RS25190 at 992.137 kb on + strand, within RR42_RS25190 at 992.137 kb on + strand, within RR42_RS25190 at 992.137 kb on + strand, within RR42_RS25190 at 992.138 kb on - strand, within RR42_RS25190 at 992.138 kb on - strand, within RR42_RS25190 at 992.159 kb on + strand, within RR42_RS25190 at 992.269 kb on + strand, within RR42_RS25190 at 992.375 kb on - strand, within RR42_RS25190 at 992.628 kb on + strand, within RR42_RS25190 at 992.629 kb on - strand, within RR42_RS25190 at 992.629 kb on - strand, within RR42_RS25190 at 992.752 kb on + strand, within RR42_RS25190 at 992.752 kb on + strand, within RR42_RS25190 at 992.753 kb on - strand, within RR42_RS25190 at 992.797 kb on + strand, within RR42_RS25190 at 992.797 kb on + strand, within RR42_RS25190 at 992.798 kb on - strand, within RR42_RS25190 at 992.841 kb on - strand, within RR42_RS25190 at 992.854 kb on - strand, within RR42_RS25190 at 992.953 kb on + strand, within RR42_RS25190 at 992.953 kb on + strand, within RR42_RS25190 at 993.078 kb on + strand at 993.079 kb on - strand at 993.184 kb on + strand at 993.184 kb on + strand at 993.185 kb on - strand at 993.185 kb on - strand at 993.185 kb on - strand at 993.187 kb on + strand at 993.188 kb on - strand at 993.188 kb on - strand at 993.188 kb on - strand at 993.195 kb on - strand at 993.195 kb on - strand at 993.263 kb on - strand at 993.353 kb on + strand at 993.353 kb on + strand at 993.372 kb on - strand at 993.372 kb on - strand at 993.869 kb on - strand, within RR42_RS25195 at 993.869 kb on - strand, within RR42_RS25195 at 993.973 kb on - strand, within RR42_RS25195 at 994.151 kb on + strand, within RR42_RS25195 at 994.151 kb on + strand, within RR42_RS25195 at 994.152 kb on - strand, within RR42_RS25195 at 994.156 kb on + strand, within RR42_RS25195 at 994.156 kb on + strand, within RR42_RS25195 at 994.156 kb on + strand, within RR42_RS25195
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.001 mg/ml remove 990,584 - RR42_RS25185 0.24 -1.9 990,587 + RR42_RS25185 0.24 -0.2 990,665 - RR42_RS25185 0.33 -1.0 990,665 - RR42_RS25185 0.33 -0.1 990,665 - RR42_RS25185 0.33 +0.7 990,715 - RR42_RS25185 0.39 +0.5 990,845 + RR42_RS25185 0.53 -0.0 990,845 + RR42_RS25185 0.53 +0.1 990,846 - RR42_RS25185 0.54 -0.1 991,184 + +0.1 991,239 - -0.6 991,260 + +0.3 991,261 - -0.4 991,273 + -0.9 991,273 + -0.8 991,273 + +0.2 991,274 - +0.1 991,274 - -0.5 991,359 + -1.0 991,360 - -0.3 991,489 - -0.2 991,489 - +0.1 991,513 + -0.1 991,513 + +0.7 991,543 + -2.4 991,544 - -1.9 991,544 - -0.0 991,544 - -0.3 991,609 - RR42_RS25190 0.10 -0.1 991,609 - RR42_RS25190 0.10 +1.3 991,636 + RR42_RS25190 0.12 +1.0 991,636 + RR42_RS25190 0.12 +0.6 991,636 + RR42_RS25190 0.12 +0.7 991,637 - RR42_RS25190 0.12 +0.2 991,637 - RR42_RS25190 0.12 +0.6 991,689 + RR42_RS25190 0.15 +1.8 991,689 + RR42_RS25190 0.15 +0.2 991,689 + RR42_RS25190 0.15 +0.3 991,899 - RR42_RS25190 0.27 -1.7 991,932 - RR42_RS25190 0.29 -0.3 991,941 - RR42_RS25190 0.29 -1.1 991,941 - RR42_RS25190 0.29 +1.8 991,942 + RR42_RS25190 0.29 -2.4 991,943 - RR42_RS25190 0.29 +0.8 991,943 - RR42_RS25190 0.29 -0.2 991,943 - RR42_RS25190 0.29 +0.2 991,943 - RR42_RS25190 0.29 +0.2 992,012 + RR42_RS25190 0.33 +0.9 992,137 + RR42_RS25190 0.40 -0.0 992,137 + RR42_RS25190 0.40 +0.4 992,137 + RR42_RS25190 0.40 -0.9 992,137 + RR42_RS25190 0.40 -1.9 992,138 - RR42_RS25190 0.40 +0.3 992,138 - RR42_RS25190 0.40 +0.9 992,159 + RR42_RS25190 0.41 +0.4 992,269 + RR42_RS25190 0.48 -0.7 992,375 - RR42_RS25190 0.54 -0.1 992,628 + RR42_RS25190 0.68 +0.8 992,629 - RR42_RS25190 0.68 +2.1 992,629 - RR42_RS25190 0.68 +0.1 992,752 + RR42_RS25190 0.75 +0.5 992,752 + RR42_RS25190 0.75 +1.2 992,753 - RR42_RS25190 0.75 -0.0 992,797 + RR42_RS25190 0.78 -0.1 992,797 + RR42_RS25190 0.78 +0.6 992,798 - RR42_RS25190 0.78 -1.9 992,841 - RR42_RS25190 0.80 +0.0 992,854 - RR42_RS25190 0.81 -1.4 992,953 + RR42_RS25190 0.86 -0.2 992,953 + RR42_RS25190 0.86 +0.6 993,078 + +0.7 993,079 - +1.4 993,184 + -2.5 993,184 + -1.3 993,185 - +0.0 993,185 - -0.9 993,185 - -0.3 993,187 + -0.6 993,188 - -0.1 993,188 - -0.4 993,188 - +0.3 993,195 - +0.3 993,195 - +0.3 993,263 - -1.0 993,353 + -0.0 993,353 + +0.4 993,372 - +0.1 993,372 - -0.1 993,869 - RR42_RS25195 0.36 +3.4 993,869 - RR42_RS25195 0.36 -0.1 993,973 - RR42_RS25195 0.43 -0.3 994,151 + RR42_RS25195 0.53 -0.3 994,151 + RR42_RS25195 0.53 -0.2 994,152 - RR42_RS25195 0.53 +0.0 994,156 + RR42_RS25195 0.53 -0.7 994,156 + RR42_RS25195 0.53 +0.8 994,156 + RR42_RS25195 0.53 -1.0
Or see this region's nucleotide sequence