Experiment: R2A with Thallium(I) acetate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05720 and RR42_RS05725 are separated by 25 nucleotides RR42_RS05725 and RR42_RS05730 overlap by 4 nucleotides RR42_RS05730 and RR42_RS05735 are separated by 98 nucleotides
RR42_RS05720: RR42_RS05720 - LysR family transcriptional regulator, at 1,257,752 to 1,258,687
_RS05720
RR42_RS05725: RR42_RS05725 - hypothetical protein, at 1,258,713 to 1,260,221
_RS05725
RR42_RS05730: RR42_RS05730 - DNA-directed RNA polymerase sigma-70 factor, at 1,260,218 to 1,260,838
_RS05730
RR42_RS05735: RR42_RS05735 - VWA domain-containing protein, at 1,260,937 to 1,262,451
_RS05735
Position (kb)
1258
1259
1260
1261 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1257.800 kb on - strand at 1257.800 kb on - strand at 1257.803 kb on + strand at 1257.803 kb on + strand at 1257.803 kb on + strand at 1257.804 kb on - strand at 1257.804 kb on - strand at 1257.804 kb on - strand at 1257.804 kb on - strand at 1258.208 kb on - strand, within RR42_RS05720 at 1258.323 kb on - strand, within RR42_RS05720 at 1258.436 kb on + strand, within RR42_RS05720 at 1258.520 kb on + strand, within RR42_RS05720 at 1258.521 kb on - strand, within RR42_RS05720 at 1258.558 kb on + strand, within RR42_RS05720 at 1258.558 kb on + strand, within RR42_RS05720 at 1258.558 kb on + strand, within RR42_RS05720 at 1258.559 kb on - strand, within RR42_RS05720 at 1258.559 kb on - strand, within RR42_RS05720 at 1258.559 kb on - strand, within RR42_RS05720 at 1258.559 kb on - strand, within RR42_RS05720 at 1258.559 kb on - strand, within RR42_RS05720 at 1258.578 kb on - strand, within RR42_RS05720 at 1258.578 kb on - strand, within RR42_RS05720 at 1258.715 kb on - strand at 1258.715 kb on - strand at 1258.715 kb on - strand at 1258.715 kb on - strand at 1258.715 kb on - strand at 1258.790 kb on - strand at 1258.790 kb on - strand at 1259.191 kb on - strand, within RR42_RS05725 at 1259.191 kb on - strand, within RR42_RS05725 at 1259.191 kb on - strand, within RR42_RS05725 at 1259.408 kb on + strand, within RR42_RS05725 at 1259.417 kb on + strand, within RR42_RS05725 at 1259.417 kb on + strand, within RR42_RS05725 at 1260.147 kb on + strand at 1260.147 kb on + strand at 1260.280 kb on - strand, within RR42_RS05730 at 1260.545 kb on - strand, within RR42_RS05730 at 1260.545 kb on - strand, within RR42_RS05730 at 1260.545 kb on - strand, within RR42_RS05730 at 1260.684 kb on + strand, within RR42_RS05730 at 1260.684 kb on + strand, within RR42_RS05730 at 1260.684 kb on + strand, within RR42_RS05730 at 1260.684 kb on + strand, within RR42_RS05730 at 1260.684 kb on + strand, within RR42_RS05730 at 1260.684 kb on + strand, within RR42_RS05730 at 1260.685 kb on - strand, within RR42_RS05730 at 1260.685 kb on - strand, within RR42_RS05730 at 1260.690 kb on + strand, within RR42_RS05730 at 1260.690 kb on + strand, within RR42_RS05730 at 1260.691 kb on - strand, within RR42_RS05730 at 1260.848 kb on + strand at 1260.849 kb on - strand at 1260.849 kb on - strand at 1260.849 kb on - strand at 1260.850 kb on + strand at 1260.850 kb on + strand at 1260.850 kb on + strand at 1260.851 kb on - strand at 1260.851 kb on - strand at 1260.851 kb on - strand at 1260.851 kb on - strand at 1261.091 kb on + strand, within RR42_RS05735 at 1261.126 kb on + strand, within RR42_RS05735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.001 mg/ml remove 1,257,800 - +0.2 1,257,800 - +0.0 1,257,803 + -0.4 1,257,803 + -0.1 1,257,803 + -0.0 1,257,804 - +1.7 1,257,804 - +0.1 1,257,804 - -0.7 1,257,804 - -0.9 1,258,208 - RR42_RS05720 0.49 +1.1 1,258,323 - RR42_RS05720 0.61 +0.5 1,258,436 + RR42_RS05720 0.73 -1.4 1,258,520 + RR42_RS05720 0.82 -0.3 1,258,521 - RR42_RS05720 0.82 -0.3 1,258,558 + RR42_RS05720 0.86 +0.8 1,258,558 + RR42_RS05720 0.86 -0.9 1,258,558 + RR42_RS05720 0.86 +1.1 1,258,559 - RR42_RS05720 0.86 +0.3 1,258,559 - RR42_RS05720 0.86 +1.0 1,258,559 - RR42_RS05720 0.86 -0.6 1,258,559 - RR42_RS05720 0.86 +1.1 1,258,559 - RR42_RS05720 0.86 -0.6 1,258,578 - RR42_RS05720 0.88 +1.3 1,258,578 - RR42_RS05720 0.88 +0.1 1,258,715 - -0.7 1,258,715 - -0.4 1,258,715 - -0.4 1,258,715 - -0.0 1,258,715 - -0.6 1,258,790 - +2.1 1,258,790 - +0.3 1,259,191 - RR42_RS05725 0.32 -1.6 1,259,191 - RR42_RS05725 0.32 +0.0 1,259,191 - RR42_RS05725 0.32 -0.2 1,259,408 + RR42_RS05725 0.46 -1.3 1,259,417 + RR42_RS05725 0.47 -0.5 1,259,417 + RR42_RS05725 0.47 -0.8 1,260,147 + -0.9 1,260,147 + +0.2 1,260,280 - RR42_RS05730 0.10 +0.1 1,260,545 - RR42_RS05730 0.53 +0.3 1,260,545 - RR42_RS05730 0.53 -0.3 1,260,545 - RR42_RS05730 0.53 -0.3 1,260,684 + RR42_RS05730 0.75 -0.6 1,260,684 + RR42_RS05730 0.75 -0.1 1,260,684 + RR42_RS05730 0.75 +0.8 1,260,684 + RR42_RS05730 0.75 +1.7 1,260,684 + RR42_RS05730 0.75 -0.1 1,260,684 + RR42_RS05730 0.75 -1.0 1,260,685 - RR42_RS05730 0.75 -0.0 1,260,685 - RR42_RS05730 0.75 -2.0 1,260,690 + RR42_RS05730 0.76 -1.8 1,260,690 + RR42_RS05730 0.76 -0.9 1,260,691 - RR42_RS05730 0.76 -1.2 1,260,848 + -0.3 1,260,849 - -0.6 1,260,849 - -0.7 1,260,849 - -0.2 1,260,850 + -0.4 1,260,850 + +0.1 1,260,850 + +0.7 1,260,851 - +0.6 1,260,851 - -0.8 1,260,851 - +0.1 1,260,851 - +1.2 1,261,091 + RR42_RS05735 0.10 +2.4 1,261,126 + RR42_RS05735 0.12 +0.3
Or see this region's nucleotide sequence