Experiment: R2A with Thallium(I) acetate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01210 and RR42_RS01215 are separated by 78 nucleotides RR42_RS01215 and RR42_RS01220 are separated by 30 nucleotides RR42_RS01220 and RR42_RS01225 are separated by 30 nucleotides RR42_RS01225 and RR42_RS01230 are separated by 184 nucleotides
RR42_RS01210: RR42_RS01210 - n-acetyl-gamma-glutamyl-phosphate reductase, at 275,725 to 276,672
_RS01210
RR42_RS01215: RR42_RS01215 - hypothetical protein, at 276,751 to 276,939
_RS01215
RR42_RS01220: RR42_RS01220 - exodeoxyribonuclease III, at 276,970 to 277,746
_RS01220
RR42_RS01225: RR42_RS01225 - orotate phosphoribosyltransferase, at 277,777 to 278,466
_RS01225
RR42_RS01230: RR42_RS01230 - acetoacetate decarboxylase, at 278,651 to 279,391
_RS01230
Position (kb)
276
277
278 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 276.230 kb on + strand, within RR42_RS01210 at 276.399 kb on - strand, within RR42_RS01210 at 276.450 kb on + strand, within RR42_RS01210 at 276.451 kb on - strand, within RR42_RS01210 at 276.612 kb on - strand at 276.652 kb on + strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.653 kb on - strand at 276.692 kb on - strand at 276.692 kb on - strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.733 kb on + strand at 276.734 kb on - strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.737 kb on + strand at 276.738 kb on - strand at 276.738 kb on - strand at 276.738 kb on - strand at 276.781 kb on - strand, within RR42_RS01215 at 276.784 kb on + strand, within RR42_RS01215 at 276.784 kb on + strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.785 kb on - strand, within RR42_RS01215 at 276.938 kb on - strand at 276.965 kb on - strand at 276.965 kb on - strand at 276.965 kb on - strand at 276.971 kb on + strand at 276.971 kb on + strand at 276.972 kb on - strand at 276.972 kb on - strand at 276.972 kb on - strand at 277.031 kb on + strand at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.142 kb on + strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.143 kb on - strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.421 kb on + strand, within RR42_RS01220 at 277.422 kb on - strand, within RR42_RS01220 at 277.448 kb on + strand, within RR42_RS01220 at 277.463 kb on + strand, within RR42_RS01220 at 277.548 kb on + strand, within RR42_RS01220 at 277.548 kb on + strand, within RR42_RS01220 at 277.549 kb on - strand, within RR42_RS01220 at 277.568 kb on + strand, within RR42_RS01220 at 277.577 kb on + strand, within RR42_RS01220 at 277.577 kb on + strand, within RR42_RS01220 at 277.734 kb on + strand at 278.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Thallium(I) acetate 0.001 mg/ml remove 276,230 + RR42_RS01210 0.53 +0.5 276,399 - RR42_RS01210 0.71 -0.9 276,450 + RR42_RS01210 0.76 +1.4 276,451 - RR42_RS01210 0.77 +0.1 276,612 - +0.8 276,652 + -2.4 276,653 - -0.4 276,653 - +0.4 276,653 - +0.9 276,653 - +0.7 276,692 - -0.0 276,692 - +0.5 276,733 + -1.0 276,733 + -1.5 276,733 + +2.0 276,733 + -0.4 276,734 - +0.6 276,737 + +2.0 276,737 + -0.0 276,737 + +0.9 276,737 + +0.4 276,737 + -0.2 276,737 + -0.8 276,737 + +1.0 276,738 - -0.2 276,738 - -1.0 276,738 - -0.4 276,781 - RR42_RS01215 0.16 -0.8 276,784 + RR42_RS01215 0.17 +0.0 276,784 + RR42_RS01215 0.17 -1.0 276,785 - RR42_RS01215 0.18 -1.5 276,785 - RR42_RS01215 0.18 -0.9 276,785 - RR42_RS01215 0.18 +0.1 276,938 - +0.0 276,965 - -0.4 276,965 - -0.6 276,965 - -0.4 276,971 + -0.4 276,971 + -0.0 276,972 - +1.6 276,972 - +1.2 276,972 - -0.1 277,031 + -1.1 277,142 + RR42_RS01220 0.22 +0.3 277,142 + RR42_RS01220 0.22 +2.0 277,142 + RR42_RS01220 0.22 -1.2 277,142 + RR42_RS01220 0.22 -0.6 277,142 + RR42_RS01220 0.22 +1.1 277,143 - RR42_RS01220 0.22 -0.3 277,143 - RR42_RS01220 0.22 +0.7 277,143 - RR42_RS01220 0.22 +1.0 277,143 - RR42_RS01220 0.22 +0.3 277,143 - RR42_RS01220 0.22 -0.5 277,421 + RR42_RS01220 0.58 +1.3 277,421 + RR42_RS01220 0.58 +1.6 277,421 + RR42_RS01220 0.58 -1.2 277,422 - RR42_RS01220 0.58 -1.1 277,448 + RR42_RS01220 0.62 -2.2 277,463 + RR42_RS01220 0.63 +0.7 277,548 + RR42_RS01220 0.74 +0.2 277,548 + RR42_RS01220 0.74 -0.4 277,549 - RR42_RS01220 0.75 +1.0 277,568 + RR42_RS01220 0.77 +0.0 277,577 + RR42_RS01220 0.78 +0.5 277,577 + RR42_RS01220 0.78 +0.7 277,734 + +0.4 278,696 - -0.7
Or see this region's nucleotide sequence