Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_866

Experiment: D-Galactose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_864 and Pf6N2E2_865 are separated by 3 nucleotidesPf6N2E2_865 and Pf6N2E2_866 are separated by 22 nucleotidesPf6N2E2_866 and Pf6N2E2_867 are separated by 275 nucleotides Pf6N2E2_864 - 2-keto-4-pentenoate hydratase (EC 4.2.1.80), at 1,012,725 to 1,013,525 _864 Pf6N2E2_865 - Fumarylacetoacetate hydrolase family protein, at 1,013,529 to 1,014,422 _865 Pf6N2E2_866 - hypothetical protein, at 1,014,445 to 1,015,140 _866 Pf6N2E2_867 - PhaK-like protein, at 1,015,416 to 1,016,696 _867 Position (kb) 1014 1015 1016Strain fitness (log2 ratio) -1 0 1 2 3at 1013.537 kb on - strandat 1013.537 kb on - strandat 1013.767 kb on - strand, within Pf6N2E2_865at 1014.195 kb on - strand, within Pf6N2E2_865at 1014.204 kb on + strand, within Pf6N2E2_865at 1014.366 kb on + strandat 1014.366 kb on + strandat 1014.367 kb on - strandat 1014.367 kb on - strandat 1014.444 kb on + strandat 1014.468 kb on - strandat 1014.468 kb on - strandat 1014.514 kb on + strandat 1014.514 kb on + strandat 1014.515 kb on - strand, within Pf6N2E2_866at 1014.515 kb on - strand, within Pf6N2E2_866at 1014.515 kb on - strand, within Pf6N2E2_866at 1014.515 kb on - strand, within Pf6N2E2_866at 1014.611 kb on - strand, within Pf6N2E2_866at 1014.611 kb on - strand, within Pf6N2E2_866at 1014.622 kb on + strand, within Pf6N2E2_866at 1014.622 kb on + strand, within Pf6N2E2_866at 1014.622 kb on + strand, within Pf6N2E2_866at 1014.661 kb on + strand, within Pf6N2E2_866at 1014.661 kb on + strand, within Pf6N2E2_866at 1014.661 kb on + strand, within Pf6N2E2_866at 1014.751 kb on + strand, within Pf6N2E2_866at 1014.751 kb on + strand, within Pf6N2E2_866at 1014.778 kb on + strand, within Pf6N2E2_866at 1014.819 kb on + strand, within Pf6N2E2_866at 1014.819 kb on + strand, within Pf6N2E2_866at 1014.819 kb on + strand, within Pf6N2E2_866at 1014.820 kb on - strand, within Pf6N2E2_866at 1014.820 kb on - strand, within Pf6N2E2_866at 1014.879 kb on + strand, within Pf6N2E2_866at 1014.882 kb on + strand, within Pf6N2E2_866at 1014.882 kb on + strand, within Pf6N2E2_866at 1014.883 kb on - strand, within Pf6N2E2_866at 1015.009 kb on + strand, within Pf6N2E2_866at 1015.010 kb on - strand, within Pf6N2E2_866at 1015.093 kb on + strandat 1015.116 kb on + strandat 1015.210 kb on + strandat 1015.210 kb on + strandat 1015.210 kb on + strandat 1015.269 kb on - strandat 1015.277 kb on - strandat 1015.394 kb on + strandat 1015.572 kb on + strand, within Pf6N2E2_867at 1015.572 kb on + strand, within Pf6N2E2_867at 1015.708 kb on - strand, within Pf6N2E2_867at 1015.708 kb on - strand, within Pf6N2E2_867

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galactose (C)
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1,013,537 - +3.2
1,013,537 - +0.7
1,013,767 - Pf6N2E2_865 0.27 +0.8
1,014,195 - Pf6N2E2_865 0.74 +1.2
1,014,204 + Pf6N2E2_865 0.76 -0.1
1,014,366 + +2.3
1,014,366 + +2.5
1,014,367 - +0.5
1,014,367 - -0.3
1,014,444 + +0.5
1,014,468 - +0.2
1,014,468 - +0.6
1,014,514 + -1.2
1,014,514 + +0.6
1,014,515 - Pf6N2E2_866 0.10 +0.7
1,014,515 - Pf6N2E2_866 0.10 -1.5
1,014,515 - Pf6N2E2_866 0.10 -1.7
1,014,515 - Pf6N2E2_866 0.10 -1.3
1,014,611 - Pf6N2E2_866 0.24 +1.2
1,014,611 - Pf6N2E2_866 0.24 +0.1
1,014,622 + Pf6N2E2_866 0.25 -1.0
1,014,622 + Pf6N2E2_866 0.25 +1.5
1,014,622 + Pf6N2E2_866 0.25 +0.6
1,014,661 + Pf6N2E2_866 0.31 -0.5
1,014,661 + Pf6N2E2_866 0.31 +1.2
1,014,661 + Pf6N2E2_866 0.31 +0.3
1,014,751 + Pf6N2E2_866 0.44 +2.0
1,014,751 + Pf6N2E2_866 0.44 +1.5
1,014,778 + Pf6N2E2_866 0.48 -0.3
1,014,819 + Pf6N2E2_866 0.54 +1.5
1,014,819 + Pf6N2E2_866 0.54 +1.3
1,014,819 + Pf6N2E2_866 0.54 +1.5
1,014,820 - Pf6N2E2_866 0.54 -0.4
1,014,820 - Pf6N2E2_866 0.54 -0.7
1,014,879 + Pf6N2E2_866 0.62 +2.4
1,014,882 + Pf6N2E2_866 0.63 +0.7
1,014,882 + Pf6N2E2_866 0.63 +1.4
1,014,883 - Pf6N2E2_866 0.63 +1.4
1,015,009 + Pf6N2E2_866 0.81 +1.5
1,015,010 - Pf6N2E2_866 0.81 +0.0
1,015,093 + +1.2
1,015,116 + +2.7
1,015,210 + +3.5
1,015,210 + +3.2
1,015,210 + +3.3
1,015,269 - +0.5
1,015,277 - -0.3
1,015,394 + +3.6
1,015,572 + Pf6N2E2_867 0.12 +1.0
1,015,572 + Pf6N2E2_867 0.12 -0.7
1,015,708 - Pf6N2E2_867 0.23 -1.6
1,015,708 - Pf6N2E2_867 0.23 +0.0

Or see this region's nucleotide sequence