Experiment: D-Galactose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_512 and Pf6N2E2_513 are separated by 194 nucleotides Pf6N2E2_513 and Pf6N2E2_514 overlap by 4 nucleotides Pf6N2E2_514 and Pf6N2E2_515 are separated by 30 nucleotides
Pf6N2E2_512 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data) , at 580,158 to 582,095
_512
Pf6N2E2_513 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data) , at 582,290 to 583,183
_513
Pf6N2E2_514 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data) , at 583,180 to 583,989
_514
Pf6N2E2_515 - malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (from data) , at 584,020 to 585,522
_515
Position (kb)
582
583
584 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 581.293 kb on + strand, within Pf6N2E2_512 at 581.500 kb on + strand, within Pf6N2E2_512 at 581.501 kb on - strand, within Pf6N2E2_512 at 581.658 kb on + strand, within Pf6N2E2_512 at 581.658 kb on + strand, within Pf6N2E2_512 at 581.658 kb on + strand, within Pf6N2E2_512 at 581.658 kb on + strand, within Pf6N2E2_512 at 581.659 kb on - strand, within Pf6N2E2_512 at 581.659 kb on - strand, within Pf6N2E2_512 at 581.659 kb on - strand, within Pf6N2E2_512 at 581.746 kb on - strand, within Pf6N2E2_512 at 581.767 kb on - strand, within Pf6N2E2_512 at 581.778 kb on - strand, within Pf6N2E2_512 at 582.099 kb on + strand at 582.100 kb on - strand at 582.100 kb on - strand at 582.263 kb on + strand at 582.263 kb on + strand at 582.264 kb on - strand at 582.264 kb on - strand at 582.264 kb on - strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.473 kb on + strand, within Pf6N2E2_513 at 582.501 kb on - strand, within Pf6N2E2_513 at 582.735 kb on + strand, within Pf6N2E2_513 at 582.747 kb on - strand, within Pf6N2E2_513 at 582.747 kb on - strand, within Pf6N2E2_513 at 582.767 kb on + strand, within Pf6N2E2_513 at 582.768 kb on - strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 583.064 kb on + strand, within Pf6N2E2_513 at 583.107 kb on + strand at 583.108 kb on - strand at 583.118 kb on + strand at 583.118 kb on + strand at 583.118 kb on + strand at 583.118 kb on + strand at 583.119 kb on - strand at 583.119 kb on - strand at 583.140 kb on - strand at 583.249 kb on + strand at 583.249 kb on + strand at 583.249 kb on + strand at 583.250 kb on - strand at 583.250 kb on - strand at 583.435 kb on + strand, within Pf6N2E2_514 at 583.436 kb on - strand, within Pf6N2E2_514 at 583.490 kb on + strand, within Pf6N2E2_514 at 583.491 kb on - strand, within Pf6N2E2_514 at 583.534 kb on + strand, within Pf6N2E2_514 at 583.535 kb on - strand, within Pf6N2E2_514 at 583.535 kb on - strand, within Pf6N2E2_514 at 583.604 kb on - strand, within Pf6N2E2_514 at 583.750 kb on + strand, within Pf6N2E2_514 at 583.750 kb on + strand at 583.751 kb on - strand, within Pf6N2E2_514 at 583.751 kb on - strand, within Pf6N2E2_514 at 583.798 kb on + strand, within Pf6N2E2_514 at 583.798 kb on + strand, within Pf6N2E2_514 at 583.799 kb on - strand, within Pf6N2E2_514 at 583.799 kb on - strand, within Pf6N2E2_514 at 583.906 kb on + strand, within Pf6N2E2_514 at 583.907 kb on - strand, within Pf6N2E2_514 at 583.907 kb on - strand, within Pf6N2E2_514 at 583.909 kb on + strand at 584.003 kb on + strand at 584.047 kb on + strand at 584.047 kb on + strand at 584.048 kb on - strand at 584.048 kb on - strand at 584.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galactose (C) remove 581,293 + Pf6N2E2_512 0.59 +1.3 581,500 + Pf6N2E2_512 0.69 +0.4 581,501 - Pf6N2E2_512 0.69 -1.1 581,658 + Pf6N2E2_512 0.77 +0.5 581,658 + Pf6N2E2_512 0.77 +1.3 581,658 + Pf6N2E2_512 0.77 +0.9 581,658 + Pf6N2E2_512 0.77 +1.3 581,659 - Pf6N2E2_512 0.77 +0.5 581,659 - Pf6N2E2_512 0.77 +0.3 581,659 - Pf6N2E2_512 0.77 -0.7 581,746 - Pf6N2E2_512 0.82 -0.6 581,767 - Pf6N2E2_512 0.83 -0.4 581,778 - Pf6N2E2_512 0.84 -2.3 582,099 + +1.5 582,100 - +0.2 582,100 - +0.1 582,263 + +1.9 582,263 + +1.0 582,264 - -1.0 582,264 - +0.5 582,264 - +0.5 582,275 + +1.7 582,275 + -1.0 582,275 + +1.4 582,275 + +0.4 582,275 + +1.3 582,275 + +1.0 582,275 + +0.8 582,276 - +1.5 582,276 - -1.8 582,276 - -0.4 582,276 - +0.7 582,276 - +4.1 582,276 - +0.5 582,473 + Pf6N2E2_513 0.20 +0.7 582,501 - Pf6N2E2_513 0.24 +0.2 582,735 + Pf6N2E2_513 0.50 +1.8 582,747 - Pf6N2E2_513 0.51 +0.1 582,747 - Pf6N2E2_513 0.51 -0.6 582,767 + Pf6N2E2_513 0.53 +2.1 582,768 - Pf6N2E2_513 0.53 -0.6 582,851 + Pf6N2E2_513 0.63 +0.3 582,851 + Pf6N2E2_513 0.63 +1.9 582,851 + Pf6N2E2_513 0.63 +1.0 582,851 + Pf6N2E2_513 0.63 +0.7 582,851 + Pf6N2E2_513 0.63 +0.4 582,852 - Pf6N2E2_513 0.63 -0.8 582,852 - Pf6N2E2_513 0.63 +0.3 582,852 - Pf6N2E2_513 0.63 -0.4 582,852 - Pf6N2E2_513 0.63 -0.5 582,852 - Pf6N2E2_513 0.63 -0.6 582,852 - Pf6N2E2_513 0.63 -0.0 582,852 - Pf6N2E2_513 0.63 +1.2 583,064 + Pf6N2E2_513 0.87 +1.0 583,107 + +1.1 583,108 - -0.5 583,118 + +1.7 583,118 + +2.2 583,118 + +1.7 583,118 + +1.8 583,119 - -1.2 583,119 - +0.6 583,140 - -2.4 583,249 + +0.8 583,249 + +2.5 583,249 + +1.9 583,250 - +2.5 583,250 - -0.7 583,435 + Pf6N2E2_514 0.31 +0.3 583,436 - Pf6N2E2_514 0.32 +0.8 583,490 + Pf6N2E2_514 0.38 +0.3 583,491 - Pf6N2E2_514 0.38 -0.6 583,534 + Pf6N2E2_514 0.44 +1.3 583,535 - Pf6N2E2_514 0.44 -1.3 583,535 - Pf6N2E2_514 0.44 -1.1 583,604 - Pf6N2E2_514 0.52 -1.6 583,750 + Pf6N2E2_514 0.70 +1.4 583,750 + +1.5 583,751 - Pf6N2E2_514 0.70 -0.5 583,751 - Pf6N2E2_514 0.70 -0.8 583,798 + Pf6N2E2_514 0.76 +1.5 583,798 + Pf6N2E2_514 0.76 +2.5 583,799 - Pf6N2E2_514 0.76 +0.8 583,799 - Pf6N2E2_514 0.76 +0.2 583,906 + Pf6N2E2_514 0.90 +1.5 583,907 - Pf6N2E2_514 0.90 +0.3 583,907 - Pf6N2E2_514 0.90 -0.2 583,909 + +1.2 584,003 + +2.1 584,047 + +0.7 584,047 + +0.8 584,048 - +0.7 584,048 - -0.1 584,048 - +0.3
Or see this region's nucleotide sequence