Experiment: D-Fructose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5652 and Pf1N1B4_5653 are separated by 149 nucleotides Pf1N1B4_5653 and Pf1N1B4_5654 are separated by 3 nucleotides
Pf1N1B4_5652 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 80,438 to 81,448
_5652
Pf1N1B4_5653 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 81,598 to 85,464
_5653
Pf1N1B4_5654 - CobW GTPase involved in cobalt insertion for B12 biosynthesis, at 85,468 to 86,529
_5654
Position (kb) 81
82
83
84
85
86 Strain fitness (log2 ratio) -2
-1
0
1
2 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.171 kb on - strand, within Pf1N1B4_5652 at 81.614 kb on - strand at 81.614 kb on - strand at 81.768 kb on - strand at 81.768 kb on - strand at 81.870 kb on + strand at 81.870 kb on + strand at 81.871 kb on - strand at 81.871 kb on - strand at 82.058 kb on + strand, within Pf1N1B4_5653 at 82.778 kb on + strand at 82.951 kb on + strand, within Pf1N1B4_5653 at 82.952 kb on - strand, within Pf1N1B4_5653 at 83.311 kb on - strand, within Pf1N1B4_5653 at 83.414 kb on + strand, within Pf1N1B4_5653 at 83.414 kb on + strand, within Pf1N1B4_5653 at 83.415 kb on - strand, within Pf1N1B4_5653 at 83.415 kb on - strand, within Pf1N1B4_5653 at 83.570 kb on + strand at 83.571 kb on - strand, within Pf1N1B4_5653 at 83.612 kb on + strand, within Pf1N1B4_5653 at 83.717 kb on + strand, within Pf1N1B4_5653 at 83.717 kb on + strand, within Pf1N1B4_5653 at 83.840 kb on + strand at 83.841 kb on - strand, within Pf1N1B4_5653 at 83.862 kb on - strand at 83.862 kb on - strand, within Pf1N1B4_5653 at 83.874 kb on - strand at 84.089 kb on + strand, within Pf1N1B4_5653 at 84.972 kb on - strand, within Pf1N1B4_5653 at 85.253 kb on + strand at 85.254 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Fructose (C) remove 81,141 + Pf1N1B4_5652 0.70 +0.8 81,141 + Pf1N1B4_5652 0.70 +0.3 81,142 - Pf1N1B4_5652 0.70 +0.1 81,142 - Pf1N1B4_5652 0.70 -1.2 81,142 - Pf1N1B4_5652 0.70 -0.9 81,142 - Pf1N1B4_5652 0.70 -1.2 81,142 - Pf1N1B4_5652 0.70 +0.1 81,171 - Pf1N1B4_5652 0.73 -0.7 81,614 - -1.7 81,614 - -1.6 81,768 - +0.8 81,768 - -1.9 81,870 + -0.1 81,870 + +0.7 81,871 - -1.4 81,871 - -1.4 82,058 + Pf1N1B4_5653 0.12 +0.4 82,778 + +0.5 82,951 + Pf1N1B4_5653 0.35 -0.2 82,952 - Pf1N1B4_5653 0.35 -0.9 83,311 - Pf1N1B4_5653 0.44 -0.5 83,414 + Pf1N1B4_5653 0.47 -1.5 83,414 + Pf1N1B4_5653 0.47 -1.2 83,415 - Pf1N1B4_5653 0.47 -0.9 83,415 - Pf1N1B4_5653 0.47 -1.7 83,570 + +0.1 83,571 - Pf1N1B4_5653 0.51 +0.3 83,612 + Pf1N1B4_5653 0.52 +0.3 83,717 + Pf1N1B4_5653 0.55 +0.1 83,717 + Pf1N1B4_5653 0.55 +0.2 83,840 + +0.1 83,841 - Pf1N1B4_5653 0.58 -0.1 83,862 - +2.1 83,862 - Pf1N1B4_5653 0.59 -1.1 83,874 - +0.1 84,089 + Pf1N1B4_5653 0.64 +0.1 84,972 - Pf1N1B4_5653 0.87 +0.1 85,253 + -0.7 85,254 - -0.2
Or see this region's nucleotide sequence