Experiment: D-Fructose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5591 and Pf1N1B4_5592 are separated by 15 nucleotides Pf1N1B4_5592 and Pf1N1B4_5593 overlap by 32 nucleotides Pf1N1B4_5593 and Pf1N1B4_5594 are separated by 212 nucleotides Pf1N1B4_5594 and Pf1N1B4_5595 are separated by 57 nucleotides
Pf1N1B4_5591 - Vanillin dehydrogenase (Hydroxybenzaldehyde dehydrogenase) (EC 1.2.1.28), at 13,810 to 15,258
_5591
Pf1N1B4_5592 - hypothetical protein, at 15,274 to 15,417
_5592
Pf1N1B4_5593 - 4-hydroxycinnamoyl CoA hydratase/lyase (Enoyl-CoA hydratase/lyase) (EC 4.2.1.17), at 15,386 to 16,216
_5593
Pf1N1B4_5594 - Transcriptional regulator, MarR family, at 16,429 to 16,917
_5594
Pf1N1B4_5595 - Phenylacetic acid-specific porin; hydroxycinnamate specific porine, at 16,975 to 18,213
_5595
Position (kb) 15
16
17 Strain fitness (log2 ratio) -2
-1
0
1
2 at 14.878 kb on + strand, within Pf1N1B4_5591 at 14.878 kb on + strand at 14.878 kb on + strand, within Pf1N1B4_5591 at 14.879 kb on - strand, within Pf1N1B4_5591 at 15.273 kb on - strand at 15.291 kb on - strand, within Pf1N1B4_5592 at 15.508 kb on - strand, within Pf1N1B4_5593 at 15.708 kb on + strand, within Pf1N1B4_5593 at 15.708 kb on + strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.709 kb on - strand, within Pf1N1B4_5593 at 15.903 kb on + strand, within Pf1N1B4_5593 at 15.904 kb on - strand, within Pf1N1B4_5593 at 15.993 kb on + strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 15.994 kb on - strand, within Pf1N1B4_5593 at 16.206 kb on + strand at 16.976 kb on + strand at 16.977 kb on - strand at 17.118 kb on - strand, within Pf1N1B4_5595 at 17.118 kb on - strand at 17.198 kb on + strand at 17.198 kb on + strand, within Pf1N1B4_5595 at 17.198 kb on + strand, within Pf1N1B4_5595 at 17.199 kb on - strand, within Pf1N1B4_5595 at 17.199 kb on - strand, within Pf1N1B4_5595 at 17.199 kb on - strand, within Pf1N1B4_5595
Per-strain Table
Position Strand Gene LocusTag Fraction D-Fructose (C) remove 14,878 + Pf1N1B4_5591 0.74 -0.3 14,878 + +1.1 14,878 + Pf1N1B4_5591 0.74 +1.7 14,879 - Pf1N1B4_5591 0.74 +0.5 15,273 - +2.1 15,291 - Pf1N1B4_5592 0.12 +1.1 15,508 - Pf1N1B4_5593 0.15 +1.1 15,708 + Pf1N1B4_5593 0.39 +0.9 15,708 + Pf1N1B4_5593 0.39 +0.8 15,709 - Pf1N1B4_5593 0.39 +0.8 15,709 - Pf1N1B4_5593 0.39 -0.1 15,709 - Pf1N1B4_5593 0.39 -0.1 15,709 - Pf1N1B4_5593 0.39 +0.4 15,709 - Pf1N1B4_5593 0.39 -0.2 15,903 + Pf1N1B4_5593 0.62 -0.2 15,904 - Pf1N1B4_5593 0.62 +0.1 15,993 + Pf1N1B4_5593 0.73 -0.3 15,994 - Pf1N1B4_5593 0.73 -0.5 15,994 - Pf1N1B4_5593 0.73 +1.2 15,994 - Pf1N1B4_5593 0.73 +0.1 16,206 + -0.9 16,976 + -0.1 16,977 - +0.3 17,118 - Pf1N1B4_5595 0.12 +1.1 17,118 - -1.9 17,198 + -0.5 17,198 + Pf1N1B4_5595 0.18 -0.5 17,198 + Pf1N1B4_5595 0.18 +1.1 17,199 - Pf1N1B4_5595 0.18 -1.6 17,199 - Pf1N1B4_5595 0.18 +0.3 17,199 - Pf1N1B4_5595 0.18 +1.7
Or see this region's nucleotide sequence