Experiment: D-Fructose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1144 and Pf1N1B4_1145 are separated by 11 nucleotides Pf1N1B4_1145 and Pf1N1B4_1146 overlap by 1 nucleotides Pf1N1B4_1146 and Pf1N1B4_1147 are separated by 301 nucleotides
Pf1N1B4_1144 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 1,286,781 to 1,288,520
_1144
Pf1N1B4_1145 - 1-phosphofructokinase (EC 2.7.1.56), at 1,288,532 to 1,289,473
_1145
Pf1N1B4_1146 - fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (from data) , at 1,289,473 to 1,292,334
_1146
Pf1N1B4_1147 - Fructose repressor FruR, LacI family, at 1,292,636 to 1,293,631
_1147
Position (kb)
1289
1290
1291
1292
1293 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1288.513 kb on - strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1289.197 kb on + strand, within Pf1N1B4_1145 at 1289.489 kb on - strand at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1290.349 kb on + strand, within Pf1N1B4_1146 at 1290.349 kb on + strand, within Pf1N1B4_1146 at 1290.350 kb on - strand, within Pf1N1B4_1146 at 1290.350 kb on - strand at 1290.350 kb on - strand, within Pf1N1B4_1146 at 1290.568 kb on - strand, within Pf1N1B4_1146 at 1290.695 kb on + strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1290.696 kb on - strand, within Pf1N1B4_1146 at 1291.621 kb on + strand, within Pf1N1B4_1146 at 1291.621 kb on + strand, within Pf1N1B4_1146 at 1291.621 kb on + strand, within Pf1N1B4_1146 at 1291.621 kb on + strand, within Pf1N1B4_1146 at 1291.622 kb on - strand, within Pf1N1B4_1146 at 1291.622 kb on - strand, within Pf1N1B4_1146 at 1291.622 kb on - strand, within Pf1N1B4_1146 at 1291.622 kb on - strand, within Pf1N1B4_1146 at 1292.054 kb on + strand at 1292.055 kb on - strand at 1292.163 kb on + strand at 1292.163 kb on + strand at 1292.164 kb on - strand at 1292.164 kb on - strand at 1292.164 kb on - strand at 1292.164 kb on - strand at 1292.164 kb on - strand at 1292.219 kb on + strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.220 kb on - strand at 1292.263 kb on + strand at 1292.263 kb on + strand at 1292.263 kb on + strand at 1292.264 kb on - strand at 1292.264 kb on - strand at 1292.264 kb on - strand at 1292.317 kb on + strand at 1292.317 kb on + strand at 1292.318 kb on - strand at 1292.318 kb on - strand at 1292.368 kb on + strand at 1292.368 kb on + strand at 1292.368 kb on + strand at 1292.368 kb on + strand at 1292.369 kb on - strand at 1292.391 kb on - strand at 1292.391 kb on - strand at 1292.391 kb on - strand at 1292.473 kb on + strand at 1292.617 kb on + strand at 1292.618 kb on - strand at 1292.630 kb on - strand at 1292.651 kb on - strand at 1292.651 kb on - strand at 1292.651 kb on - strand at 1292.690 kb on + strand at 1292.690 kb on + strand at 1292.690 kb on + strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.691 kb on - strand at 1292.722 kb on + strand at 1292.722 kb on + strand at 1292.722 kb on + strand at 1292.723 kb on - strand at 1292.723 kb on - strand at 1292.723 kb on - strand at 1292.861 kb on + strand, within Pf1N1B4_1147 at 1292.862 kb on - strand, within Pf1N1B4_1147 at 1292.924 kb on - strand, within Pf1N1B4_1147 at 1292.961 kb on + strand at 1292.961 kb on + strand, within Pf1N1B4_1147 at 1292.961 kb on + strand, within Pf1N1B4_1147 at 1292.961 kb on + strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1292.962 kb on - strand, within Pf1N1B4_1147 at 1293.035 kb on + strand at 1293.035 kb on + strand, within Pf1N1B4_1147 at 1293.146 kb on - strand, within Pf1N1B4_1147 at 1293.211 kb on + strand, within Pf1N1B4_1147 at 1293.211 kb on + strand, within Pf1N1B4_1147 at 1293.212 kb on - strand, within Pf1N1B4_1147
Per-strain Table
Position Strand Gene LocusTag Fraction D-Fructose (C) remove 1,288,513 - -0.2 1,288,533 + -1.2 1,288,533 + -1.4 1,288,533 + -1.4 1,288,533 + -2.3 1,288,534 - -0.6 1,288,534 - +1.6 1,288,534 - +0.3 1,288,534 - -1.6 1,288,534 - -1.4 1,288,534 - -0.7 1,289,197 + Pf1N1B4_1145 0.71 -1.2 1,289,489 - -1.1 1,289,804 + Pf1N1B4_1146 0.12 -2.0 1,289,804 + Pf1N1B4_1146 0.12 -1.9 1,289,804 + Pf1N1B4_1146 0.12 -0.0 1,289,804 + Pf1N1B4_1146 0.12 -1.2 1,289,805 - Pf1N1B4_1146 0.12 -2.1 1,289,805 - Pf1N1B4_1146 0.12 -1.0 1,289,805 - Pf1N1B4_1146 0.12 -2.7 1,289,805 - Pf1N1B4_1146 0.12 -1.0 1,289,805 - Pf1N1B4_1146 0.12 -3.1 1,289,805 - Pf1N1B4_1146 0.12 -0.7 1,289,805 - Pf1N1B4_1146 0.12 -2.9 1,289,805 - -0.4 1,289,805 - Pf1N1B4_1146 0.12 -2.4 1,289,805 - Pf1N1B4_1146 0.12 -2.2 1,289,805 - Pf1N1B4_1146 0.12 -1.4 1,289,805 - Pf1N1B4_1146 0.12 -0.7 1,289,805 - Pf1N1B4_1146 0.12 -1.6 1,289,805 - Pf1N1B4_1146 0.12 -1.2 1,290,349 + Pf1N1B4_1146 0.31 -0.7 1,290,349 + Pf1N1B4_1146 0.31 -1.6 1,290,350 - Pf1N1B4_1146 0.31 -3.8 1,290,350 - -1.4 1,290,350 - Pf1N1B4_1146 0.31 -0.0 1,290,568 - Pf1N1B4_1146 0.38 +0.1 1,290,695 + Pf1N1B4_1146 0.43 -0.6 1,290,696 - Pf1N1B4_1146 0.43 -2.3 1,290,696 - Pf1N1B4_1146 0.43 -2.3 1,290,696 - Pf1N1B4_1146 0.43 -2.6 1,290,696 - Pf1N1B4_1146 0.43 -2.6 1,290,696 - Pf1N1B4_1146 0.43 -1.7 1,290,696 - Pf1N1B4_1146 0.43 -2.3 1,290,696 - Pf1N1B4_1146 0.43 -2.3 1,290,696 - Pf1N1B4_1146 0.43 -1.9 1,290,696 - Pf1N1B4_1146 0.43 -3.1 1,290,696 - Pf1N1B4_1146 0.43 -1.0 1,290,696 - Pf1N1B4_1146 0.43 -0.0 1,290,696 - Pf1N1B4_1146 0.43 -0.7 1,290,696 - Pf1N1B4_1146 0.43 -1.0 1,290,696 - Pf1N1B4_1146 0.43 -0.4 1,290,696 - Pf1N1B4_1146 0.43 -0.7 1,291,621 + Pf1N1B4_1146 0.75 -2.1 1,291,621 + Pf1N1B4_1146 0.75 -0.0 1,291,621 + Pf1N1B4_1146 0.75 -0.0 1,291,621 + Pf1N1B4_1146 0.75 -1.6 1,291,622 - Pf1N1B4_1146 0.75 -2.1 1,291,622 - Pf1N1B4_1146 0.75 -2.2 1,291,622 - Pf1N1B4_1146 0.75 -0.4 1,291,622 - Pf1N1B4_1146 0.75 -3.0 1,292,054 + -2.8 1,292,055 - -0.3 1,292,163 + -0.4 1,292,163 + -1.2 1,292,164 - -2.4 1,292,164 - -2.5 1,292,164 - -2.1 1,292,164 - -2.5 1,292,164 - -1.9 1,292,219 + -1.4 1,292,220 - -1.0 1,292,220 - -0.0 1,292,220 - -1.9 1,292,220 - -0.7 1,292,220 - -0.7 1,292,220 - -0.7 1,292,220 - -4.6 1,292,263 + -1.0 1,292,263 + -3.7 1,292,263 + -0.6 1,292,264 - -1.4 1,292,264 - -1.9 1,292,264 - -2.0 1,292,317 + -1.0 1,292,317 + -2.1 1,292,318 - -2.5 1,292,318 - -1.9 1,292,368 + -5.5 1,292,368 + -1.6 1,292,368 + -1.0 1,292,368 + -1.6 1,292,369 - -2.3 1,292,391 - +1.3 1,292,391 - +0.1 1,292,391 - +0.9 1,292,473 + -1.0 1,292,617 + -1.9 1,292,618 - +0.4 1,292,630 - +1.1 1,292,651 - +0.9 1,292,651 - +1.1 1,292,651 - +1.0 1,292,690 + -1.0 1,292,690 + -1.6 1,292,690 + -0.4 1,292,691 - +0.5 1,292,691 - +1.9 1,292,691 - -0.0 1,292,691 - -0.1 1,292,691 - +1.4 1,292,691 - +0.6 1,292,691 - +0.6 1,292,691 - +0.5 1,292,691 - +1.3 1,292,691 - +1.0 1,292,691 - +1.1 1,292,691 - +1.3 1,292,691 - +0.4 1,292,722 + -0.0 1,292,722 + -0.0 1,292,722 + +0.6 1,292,723 - +1.3 1,292,723 - +0.5 1,292,723 - +0.8 1,292,861 + Pf1N1B4_1147 0.23 +0.4 1,292,862 - Pf1N1B4_1147 0.23 -0.3 1,292,924 - Pf1N1B4_1147 0.29 -0.4 1,292,961 + +3.2 1,292,961 + Pf1N1B4_1147 0.33 +0.4 1,292,961 + Pf1N1B4_1147 0.33 -1.0 1,292,961 + Pf1N1B4_1147 0.33 +0.4 1,292,962 - Pf1N1B4_1147 0.33 +0.0 1,292,962 - Pf1N1B4_1147 0.33 +1.5 1,292,962 - Pf1N1B4_1147 0.33 +2.9 1,292,962 - Pf1N1B4_1147 0.33 +1.0 1,292,962 - Pf1N1B4_1147 0.33 -0.1 1,292,962 - Pf1N1B4_1147 0.33 +0.5 1,293,035 + -0.0 1,293,035 + Pf1N1B4_1147 0.40 -0.0 1,293,146 - Pf1N1B4_1147 0.51 +0.7 1,293,211 + Pf1N1B4_1147 0.58 +0.6 1,293,211 + Pf1N1B4_1147 0.58 -0.1 1,293,212 - Pf1N1B4_1147 0.58 +1.3
Or see this region's nucleotide sequence