Experiment: D-Fructose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1144 and Pf1N1B4_1145 are separated by 11 nucleotides Pf1N1B4_1145 and Pf1N1B4_1146 overlap by 1 nucleotides
Pf1N1B4_1144 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 1,286,781 to 1,288,520
_1144
Pf1N1B4_1145 - 1-phosphofructokinase (EC 2.7.1.56), at 1,288,532 to 1,289,473
_1145
Pf1N1B4_1146 - fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (from data) , at 1,289,473 to 1,292,334
_1146
Position (kb)
1288
1289
1290 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1287.619 kb on + strand, within Pf1N1B4_1144 at 1287.619 kb on + strand, within Pf1N1B4_1144 at 1287.620 kb on - strand, within Pf1N1B4_1144 at 1287.620 kb on - strand, within Pf1N1B4_1144 at 1287.620 kb on - strand at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.802 kb on + strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1287.803 kb on - strand, within Pf1N1B4_1144 at 1288.213 kb on + strand, within Pf1N1B4_1144 at 1288.214 kb on - strand, within Pf1N1B4_1144 at 1288.214 kb on - strand, within Pf1N1B4_1144 at 1288.214 kb on - strand, within Pf1N1B4_1144 at 1288.316 kb on + strand, within Pf1N1B4_1144 at 1288.316 kb on + strand, within Pf1N1B4_1144 at 1288.316 kb on + strand, within Pf1N1B4_1144 at 1288.316 kb on + strand, within Pf1N1B4_1144 at 1288.317 kb on - strand, within Pf1N1B4_1144 at 1288.513 kb on - strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.533 kb on + strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1288.534 kb on - strand at 1289.197 kb on + strand, within Pf1N1B4_1145 at 1289.489 kb on - strand at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.804 kb on + strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1289.805 kb on - strand, within Pf1N1B4_1146 at 1290.349 kb on + strand, within Pf1N1B4_1146 at 1290.349 kb on + strand, within Pf1N1B4_1146 at 1290.350 kb on - strand, within Pf1N1B4_1146 at 1290.350 kb on - strand at 1290.350 kb on - strand, within Pf1N1B4_1146
Per-strain Table
Position Strand Gene LocusTag Fraction D-Fructose (C) remove 1,287,619 + Pf1N1B4_1144 0.48 -1.2 1,287,619 + Pf1N1B4_1144 0.48 -0.9 1,287,620 - Pf1N1B4_1144 0.48 -0.4 1,287,620 - Pf1N1B4_1144 0.48 -0.6 1,287,620 - -0.4 1,287,802 + Pf1N1B4_1144 0.59 -2.1 1,287,802 + Pf1N1B4_1144 0.59 -0.4 1,287,802 + Pf1N1B4_1144 0.59 -1.4 1,287,802 + -0.7 1,287,802 + Pf1N1B4_1144 0.59 +0.8 1,287,802 + Pf1N1B4_1144 0.59 -1.2 1,287,802 + Pf1N1B4_1144 0.59 -1.2 1,287,802 + Pf1N1B4_1144 0.59 -1.7 1,287,803 - Pf1N1B4_1144 0.59 -1.4 1,287,803 - Pf1N1B4_1144 0.59 -0.4 1,287,803 - Pf1N1B4_1144 0.59 -0.8 1,287,803 - Pf1N1B4_1144 0.59 -2.5 1,287,803 - Pf1N1B4_1144 0.59 -0.7 1,288,213 + Pf1N1B4_1144 0.82 +0.6 1,288,214 - Pf1N1B4_1144 0.82 -1.4 1,288,214 - Pf1N1B4_1144 0.82 -0.7 1,288,214 - Pf1N1B4_1144 0.82 -2.1 1,288,316 + Pf1N1B4_1144 0.88 +1.6 1,288,316 + Pf1N1B4_1144 0.88 -1.5 1,288,316 + Pf1N1B4_1144 0.88 -2.7 1,288,316 + Pf1N1B4_1144 0.88 -2.1 1,288,317 - Pf1N1B4_1144 0.88 -1.7 1,288,513 - -0.2 1,288,533 + -1.2 1,288,533 + -1.4 1,288,533 + -1.4 1,288,533 + -2.3 1,288,534 - -0.6 1,288,534 - +1.6 1,288,534 - +0.3 1,288,534 - -1.6 1,288,534 - -1.4 1,288,534 - -0.7 1,289,197 + Pf1N1B4_1145 0.71 -1.2 1,289,489 - -1.1 1,289,804 + Pf1N1B4_1146 0.12 -2.0 1,289,804 + Pf1N1B4_1146 0.12 -1.9 1,289,804 + Pf1N1B4_1146 0.12 -0.0 1,289,804 + Pf1N1B4_1146 0.12 -1.2 1,289,805 - Pf1N1B4_1146 0.12 -2.1 1,289,805 - Pf1N1B4_1146 0.12 -1.0 1,289,805 - Pf1N1B4_1146 0.12 -2.7 1,289,805 - Pf1N1B4_1146 0.12 -1.0 1,289,805 - Pf1N1B4_1146 0.12 -3.1 1,289,805 - Pf1N1B4_1146 0.12 -0.7 1,289,805 - Pf1N1B4_1146 0.12 -2.9 1,289,805 - -0.4 1,289,805 - Pf1N1B4_1146 0.12 -2.4 1,289,805 - Pf1N1B4_1146 0.12 -2.2 1,289,805 - Pf1N1B4_1146 0.12 -1.4 1,289,805 - Pf1N1B4_1146 0.12 -0.7 1,289,805 - Pf1N1B4_1146 0.12 -1.6 1,289,805 - Pf1N1B4_1146 0.12 -1.2 1,290,349 + Pf1N1B4_1146 0.31 -0.7 1,290,349 + Pf1N1B4_1146 0.31 -1.6 1,290,350 - Pf1N1B4_1146 0.31 -3.8 1,290,350 - -1.4 1,290,350 - Pf1N1B4_1146 0.31 -0.0
Or see this region's nucleotide sequence