Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33725 and RR42_RS33730 are separated by 190 nucleotides RR42_RS33730 and RR42_RS33735 are separated by 43 nucleotides
RR42_RS33725: RR42_RS33725 - transketolase, at 2,993,059 to 2,994,060
_RS33725
RR42_RS33730: RR42_RS33730 - hypothetical protein, at 2,994,251 to 2,995,537
_RS33730
RR42_RS33735: RR42_RS33735 - pyruvate kinase, at 2,995,581 to 2,997,011
_RS33735
Position (kb)
2994
2995
2996 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2993.267 kb on - strand, within RR42_RS33725 at 2993.269 kb on + strand, within RR42_RS33725 at 2993.270 kb on - strand, within RR42_RS33725 at 2993.353 kb on + strand, within RR42_RS33725 at 2993.353 kb on + strand, within RR42_RS33725 at 2993.353 kb on + strand, within RR42_RS33725 at 2993.354 kb on - strand, within RR42_RS33725 at 2993.354 kb on - strand, within RR42_RS33725 at 2993.354 kb on - strand, within RR42_RS33725 at 2993.596 kb on + strand, within RR42_RS33725 at 2993.597 kb on - strand, within RR42_RS33725 at 2993.641 kb on + strand, within RR42_RS33725 at 2993.641 kb on + strand, within RR42_RS33725 at 2993.642 kb on - strand, within RR42_RS33725 at 2994.053 kb on + strand at 2994.112 kb on + strand at 2994.113 kb on - strand at 2994.288 kb on + strand at 2994.288 kb on + strand at 2994.289 kb on - strand at 2994.289 kb on - strand at 2994.326 kb on + strand at 2994.337 kb on - strand at 2994.337 kb on - strand at 2994.449 kb on + strand, within RR42_RS33730 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.777 kb on + strand, within RR42_RS33730 at 2994.778 kb on - strand, within RR42_RS33730 at 2994.778 kb on - strand, within RR42_RS33730 at 2994.984 kb on - strand, within RR42_RS33730 at 2995.147 kb on - strand, within RR42_RS33730 at 2995.437 kb on + strand at 2995.438 kb on - strand at 2995.502 kb on + strand at 2995.503 kb on - strand at 2995.717 kb on + strand at 2995.718 kb on - strand at 2995.882 kb on + strand, within RR42_RS33735 at 2995.899 kb on + strand, within RR42_RS33735 at 2995.997 kb on - strand, within RR42_RS33735 at 2995.997 kb on - strand, within RR42_RS33735 at 2996.110 kb on + strand, within RR42_RS33735 at 2996.110 kb on + strand, within RR42_RS33735 at 2996.111 kb on - strand, within RR42_RS33735 at 2996.468 kb on - strand, within RR42_RS33735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,993,267 - RR42_RS33725 0.21 -1.5 2,993,269 + RR42_RS33725 0.21 +0.2 2,993,270 - RR42_RS33725 0.21 -0.2 2,993,353 + RR42_RS33725 0.29 +1.0 2,993,353 + RR42_RS33725 0.29 -1.1 2,993,353 + RR42_RS33725 0.29 +0.1 2,993,354 - RR42_RS33725 0.29 +2.3 2,993,354 - RR42_RS33725 0.29 -0.9 2,993,354 - RR42_RS33725 0.29 -0.7 2,993,596 + RR42_RS33725 0.54 -0.3 2,993,597 - RR42_RS33725 0.54 +0.4 2,993,641 + RR42_RS33725 0.58 -1.8 2,993,641 + RR42_RS33725 0.58 -1.9 2,993,642 - RR42_RS33725 0.58 +1.0 2,994,053 + -0.0 2,994,112 + -0.1 2,994,113 - -1.7 2,994,288 + -1.2 2,994,288 + -2.0 2,994,289 - +0.8 2,994,289 - -0.3 2,994,326 + -0.3 2,994,337 - -2.1 2,994,337 - +0.4 2,994,449 + RR42_RS33730 0.15 -1.0 2,994,777 + RR42_RS33730 0.41 +0.0 2,994,777 + RR42_RS33730 0.41 +2.9 2,994,777 + RR42_RS33730 0.41 +1.5 2,994,778 - RR42_RS33730 0.41 +0.8 2,994,778 - RR42_RS33730 0.41 -0.9 2,994,984 - RR42_RS33730 0.57 -0.2 2,995,147 - RR42_RS33730 0.70 -0.6 2,995,437 + +0.2 2,995,438 - +0.1 2,995,502 + -0.5 2,995,503 - +0.1 2,995,717 + +1.0 2,995,718 - +0.7 2,995,882 + RR42_RS33735 0.21 +0.6 2,995,899 + RR42_RS33735 0.22 -0.3 2,995,997 - RR42_RS33735 0.29 -0.2 2,995,997 - RR42_RS33735 0.29 -0.6 2,996,110 + RR42_RS33735 0.37 +2.0 2,996,110 + RR42_RS33735 0.37 -0.0 2,996,111 - RR42_RS33735 0.37 -0.3 2,996,468 - RR42_RS33735 0.62 -1.4
Or see this region's nucleotide sequence