Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33065 and RR42_RS33070 are separated by 140 nucleotides RR42_RS33070 and RR42_RS33075 are separated by 135 nucleotides
RR42_RS33065: RR42_RS33065 - mandelate racemase, at 2,824,157 to 2,825,329
_RS33065
RR42_RS33070: RR42_RS33070 - oxidoreductase, at 2,825,470 to 2,826,171
_RS33070
RR42_RS33075: RR42_RS33075 - dihydrodipicolinate synthetase, at 2,826,307 to 2,827,248
_RS33075
Position (kb)
2825
2826
2827 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2824.560 kb on + strand, within RR42_RS33065 at 2824.564 kb on - strand, within RR42_RS33065 at 2824.590 kb on + strand, within RR42_RS33065 at 2824.591 kb on - strand, within RR42_RS33065 at 2824.591 kb on - strand, within RR42_RS33065 at 2824.591 kb on - strand, within RR42_RS33065 at 2824.653 kb on + strand, within RR42_RS33065 at 2824.812 kb on + strand, within RR42_RS33065 at 2824.860 kb on + strand, within RR42_RS33065 at 2824.860 kb on + strand, within RR42_RS33065 at 2824.860 kb on + strand, within RR42_RS33065 at 2824.860 kb on + strand, within RR42_RS33065 at 2824.860 kb on + strand, within RR42_RS33065 at 2824.861 kb on - strand, within RR42_RS33065 at 2824.861 kb on - strand, within RR42_RS33065 at 2824.861 kb on - strand, within RR42_RS33065 at 2824.879 kb on - strand, within RR42_RS33065 at 2825.117 kb on - strand, within RR42_RS33065 at 2825.157 kb on + strand, within RR42_RS33065 at 2825.262 kb on + strand at 2825.263 kb on - strand at 2825.348 kb on + strand at 2825.348 kb on + strand at 2825.349 kb on - strand at 2825.349 kb on - strand at 2825.393 kb on + strand at 2825.393 kb on + strand at 2825.394 kb on - strand at 2825.394 kb on - strand at 2825.575 kb on - strand, within RR42_RS33070 at 2825.744 kb on + strand, within RR42_RS33070 at 2826.026 kb on - strand, within RR42_RS33070 at 2826.486 kb on - strand, within RR42_RS33075 at 2826.647 kb on + strand, within RR42_RS33075 at 2826.648 kb on - strand, within RR42_RS33075 at 2826.717 kb on - strand, within RR42_RS33075 at 2826.717 kb on - strand, within RR42_RS33075 at 2826.758 kb on + strand, within RR42_RS33075 at 2826.758 kb on + strand, within RR42_RS33075 at 2826.759 kb on - strand, within RR42_RS33075 at 2826.797 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.821 kb on + strand, within RR42_RS33075 at 2826.822 kb on - strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.825 kb on + strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.826 kb on - strand, within RR42_RS33075 at 2826.872 kb on + strand, within RR42_RS33075 at 2826.872 kb on + strand, within RR42_RS33075 at 2826.872 kb on + strand, within RR42_RS33075 at 2827.101 kb on + strand, within RR42_RS33075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,824,560 + RR42_RS33065 0.34 -0.8 2,824,564 - RR42_RS33065 0.35 -1.6 2,824,590 + RR42_RS33065 0.37 +0.1 2,824,591 - RR42_RS33065 0.37 -0.1 2,824,591 - RR42_RS33065 0.37 -0.4 2,824,591 - RR42_RS33065 0.37 +0.9 2,824,653 + RR42_RS33065 0.42 -1.0 2,824,812 + RR42_RS33065 0.56 -0.8 2,824,860 + RR42_RS33065 0.60 -0.6 2,824,860 + RR42_RS33065 0.60 -0.9 2,824,860 + RR42_RS33065 0.60 -0.5 2,824,860 + RR42_RS33065 0.60 -1.0 2,824,860 + RR42_RS33065 0.60 -0.9 2,824,861 - RR42_RS33065 0.60 -0.0 2,824,861 - RR42_RS33065 0.60 +0.1 2,824,861 - RR42_RS33065 0.60 -0.8 2,824,879 - RR42_RS33065 0.62 +0.8 2,825,117 - RR42_RS33065 0.82 -0.4 2,825,157 + RR42_RS33065 0.85 +0.1 2,825,262 + +0.4 2,825,263 - +0.7 2,825,348 + +0.0 2,825,348 + -0.6 2,825,349 - +0.7 2,825,349 - +0.4 2,825,393 + +0.9 2,825,393 + +2.7 2,825,394 - +0.3 2,825,394 - +0.1 2,825,575 - RR42_RS33070 0.15 +0.6 2,825,744 + RR42_RS33070 0.39 -0.3 2,826,026 - RR42_RS33070 0.79 +2.6 2,826,486 - RR42_RS33075 0.19 -1.5 2,826,647 + RR42_RS33075 0.36 -0.8 2,826,648 - RR42_RS33075 0.36 +0.2 2,826,717 - RR42_RS33075 0.44 -1.8 2,826,717 - RR42_RS33075 0.44 -0.5 2,826,758 + RR42_RS33075 0.48 +0.1 2,826,758 + RR42_RS33075 0.48 +0.2 2,826,759 - RR42_RS33075 0.48 -0.3 2,826,797 + RR42_RS33075 0.52 +2.2 2,826,821 + RR42_RS33075 0.55 -0.5 2,826,821 + RR42_RS33075 0.55 -0.1 2,826,821 + RR42_RS33075 0.55 +0.9 2,826,821 + RR42_RS33075 0.55 +0.8 2,826,821 + RR42_RS33075 0.55 +0.2 2,826,822 - RR42_RS33075 0.55 +0.2 2,826,825 + RR42_RS33075 0.55 +0.1 2,826,825 + RR42_RS33075 0.55 +0.4 2,826,825 + RR42_RS33075 0.55 +0.4 2,826,825 + RR42_RS33075 0.55 -0.4 2,826,825 + RR42_RS33075 0.55 -0.9 2,826,825 + RR42_RS33075 0.55 +0.2 2,826,825 + RR42_RS33075 0.55 -0.2 2,826,825 + RR42_RS33075 0.55 +0.3 2,826,825 + RR42_RS33075 0.55 +0.1 2,826,826 - RR42_RS33075 0.55 -0.6 2,826,826 - RR42_RS33075 0.55 +0.5 2,826,826 - RR42_RS33075 0.55 +1.2 2,826,826 - RR42_RS33075 0.55 +0.2 2,826,826 - RR42_RS33075 0.55 +0.7 2,826,872 + RR42_RS33075 0.60 +0.0 2,826,872 + RR42_RS33075 0.60 -0.1 2,826,872 + RR42_RS33075 0.60 -0.6 2,827,101 + RR42_RS33075 0.84 +0.7
Or see this region's nucleotide sequence