Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32650 and RR42_RS32655 are separated by 144 nucleotides RR42_RS32655 and RR42_RS32660 are separated by 85 nucleotides RR42_RS32660 and RR42_RS32665 are separated by 13 nucleotides
RR42_RS32650: RR42_RS32650 - betaine-aldehyde dehydrogenase, at 2,721,394 to 2,722,848
_RS32650
RR42_RS32655: RR42_RS32655 - catechol 2,3-dioxygenase, at 2,722,993 to 2,723,925
_RS32655
RR42_RS32660: RR42_RS32660 - ferredoxin, at 2,724,011 to 2,724,358
_RS32660
RR42_RS32665: RR42_RS32665 - phenol hydroxylase, at 2,724,372 to 2,725,439
_RS32665
Position (kb)
2722
2723
2724 Strain fitness (log2 ratio)
-1
0
1 at 2722.111 kb on + strand, within RR42_RS32650 at 2722.149 kb on + strand, within RR42_RS32650 at 2722.244 kb on + strand, within RR42_RS32650 at 2722.440 kb on - strand, within RR42_RS32650 at 2722.440 kb on - strand, within RR42_RS32650 at 2722.648 kb on + strand, within RR42_RS32650 at 2722.648 kb on + strand, within RR42_RS32650 at 2723.002 kb on + strand at 2723.003 kb on - strand at 2723.153 kb on + strand, within RR42_RS32655 at 2723.172 kb on + strand, within RR42_RS32655 at 2723.723 kb on + strand, within RR42_RS32655 at 2723.724 kb on - strand, within RR42_RS32655 at 2723.796 kb on - strand, within RR42_RS32655 at 2723.964 kb on + strand at 2724.012 kb on + strand at 2724.321 kb on + strand, within RR42_RS32660 at 2724.353 kb on - strand at 2724.353 kb on - strand at 2724.518 kb on - strand, within RR42_RS32665 at 2724.560 kb on - strand, within RR42_RS32665 at 2724.643 kb on + strand, within RR42_RS32665 at 2724.694 kb on + strand, within RR42_RS32665 at 2724.694 kb on + strand, within RR42_RS32665 at 2724.695 kb on - strand, within RR42_RS32665 at 2724.856 kb on + strand, within RR42_RS32665
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,722,111 + RR42_RS32650 0.49 +0.2 2,722,149 + RR42_RS32650 0.52 +0.1 2,722,244 + RR42_RS32650 0.58 -0.4 2,722,440 - RR42_RS32650 0.72 +1.7 2,722,440 - RR42_RS32650 0.72 +0.2 2,722,648 + RR42_RS32650 0.86 +0.8 2,722,648 + RR42_RS32650 0.86 -0.3 2,723,002 + +0.6 2,723,003 - +1.3 2,723,153 + RR42_RS32655 0.17 -0.9 2,723,172 + RR42_RS32655 0.19 -1.1 2,723,723 + RR42_RS32655 0.78 -0.4 2,723,724 - RR42_RS32655 0.78 -1.5 2,723,796 - RR42_RS32655 0.86 -0.1 2,723,964 + -0.3 2,724,012 + -0.3 2,724,321 + RR42_RS32660 0.89 +1.6 2,724,353 - -0.8 2,724,353 - -1.0 2,724,518 - RR42_RS32665 0.14 -0.4 2,724,560 - RR42_RS32665 0.18 +0.1 2,724,643 + RR42_RS32665 0.25 -0.2 2,724,694 + RR42_RS32665 0.30 +0.2 2,724,694 + RR42_RS32665 0.30 +0.8 2,724,695 - RR42_RS32665 0.30 +0.7 2,724,856 + RR42_RS32665 0.45 +0.5
Or see this region's nucleotide sequence