Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30285 and RR42_RS30290 are separated by 16 nucleotides RR42_RS30290 and RR42_RS30295 are separated by 68 nucleotides RR42_RS30295 and RR42_RS30300 are separated by 31 nucleotides RR42_RS30300 and RR42_RS30305 overlap by 4 nucleotides
RR42_RS30285: RR42_RS30285 - amidohydrolase, at 2,172,164 to 2,174,014
_RS30285
RR42_RS30290: RR42_RS30290 - hypothetical protein, at 2,174,031 to 2,174,471
_RS30290
RR42_RS30295: RR42_RS30295 - ABC transporter substrate-binding protein, at 2,174,540 to 2,174,836
_RS30295
RR42_RS30300: RR42_RS30300 - toxin, at 2,174,868 to 2,175,383
_RS30300
RR42_RS30305: RR42_RS30305 - regulator, at 2,175,380 to 2,175,709
_RS30305
Position (kb)
2174
2175 Strain fitness (log2 ratio)
-1
0
1 at 2173.775 kb on + strand, within RR42_RS30285 at 2173.775 kb on + strand, within RR42_RS30285 at 2173.972 kb on + strand at 2173.972 kb on + strand at 2173.972 kb on + strand at 2173.972 kb on + strand at 2174.549 kb on + strand at 2174.549 kb on + strand at 2174.550 kb on - strand at 2174.550 kb on - strand at 2174.552 kb on - strand at 2174.552 kb on - strand at 2174.591 kb on + strand, within RR42_RS30295 at 2174.591 kb on + strand, within RR42_RS30295 at 2174.592 kb on - strand, within RR42_RS30295 at 2174.592 kb on - strand, within RR42_RS30295 at 2174.592 kb on - strand, within RR42_RS30295 at 2174.799 kb on + strand, within RR42_RS30295 at 2174.940 kb on + strand, within RR42_RS30300 at 2175.057 kb on + strand, within RR42_RS30300 at 2175.057 kb on + strand, within RR42_RS30300 at 2175.057 kb on + strand, within RR42_RS30300 at 2175.058 kb on - strand, within RR42_RS30300 at 2175.254 kb on - strand, within RR42_RS30300 at 2175.601 kb on - strand, within RR42_RS30305 at 2175.831 kb on + strand at 2175.831 kb on + strand at 2175.831 kb on + strand at 2175.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,173,775 + RR42_RS30285 0.87 -0.1 2,173,775 + RR42_RS30285 0.87 +1.3 2,173,972 + +0.9 2,173,972 + -0.8 2,173,972 + -0.4 2,173,972 + -0.1 2,174,549 + +0.4 2,174,549 + -0.4 2,174,550 - +0.1 2,174,550 - +0.6 2,174,552 - +0.5 2,174,552 - -1.3 2,174,591 + RR42_RS30295 0.17 +0.7 2,174,591 + RR42_RS30295 0.17 -0.0 2,174,592 - RR42_RS30295 0.18 -0.7 2,174,592 - RR42_RS30295 0.18 +0.9 2,174,592 - RR42_RS30295 0.18 +1.6 2,174,799 + RR42_RS30295 0.87 -0.9 2,174,940 + RR42_RS30300 0.14 +0.9 2,175,057 + RR42_RS30300 0.37 +0.7 2,175,057 + RR42_RS30300 0.37 -0.5 2,175,057 + RR42_RS30300 0.37 +0.5 2,175,058 - RR42_RS30300 0.37 -0.8 2,175,254 - RR42_RS30300 0.75 -1.1 2,175,601 - RR42_RS30305 0.67 -0.4 2,175,831 + +0.1 2,175,831 + -0.9 2,175,831 + -1.1 2,175,832 - -1.7
Or see this region's nucleotide sequence