Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27985 and RR42_RS27990 are separated by 196 nucleotides RR42_RS27990 and RR42_RS27995 overlap by 11 nucleotides RR42_RS27995 and RR42_RS28000 are separated by 23 nucleotides RR42_RS28000 and RR42_RS28005 are separated by 129 nucleotides
RR42_RS27985: RR42_RS27985 - membrane protein, at 1,643,426 to 1,644,076
_RS27985
RR42_RS27990: RR42_RS27990 - hypothetical protein, at 1,644,273 to 1,644,527
_RS27990
RR42_RS27995: RR42_RS27995 - RelE toxin, at 1,644,517 to 1,644,807
_RS27995
RR42_RS28000: RR42_RS28000 - hypothetical protein, at 1,644,831 to 1,645,076
_RS28000
RR42_RS28005: RR42_RS28005 - phosphonate/organophosphate ester transporter subunit, at 1,645,206 to 1,646,045
_RS28005
Position (kb)
1644
1645
1646 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1643.906 kb on - strand, within RR42_RS27985 at 1644.252 kb on + strand at 1644.253 kb on - strand at 1644.272 kb on + strand at 1644.272 kb on + strand at 1644.273 kb on - strand at 1644.273 kb on - strand at 1644.273 kb on - strand at 1644.295 kb on - strand at 1644.295 kb on - strand at 1644.523 kb on + strand at 1644.523 kb on + strand at 1644.524 kb on - strand at 1644.674 kb on - strand, within RR42_RS27995 at 1644.674 kb on - strand, within RR42_RS27995 at 1644.713 kb on - strand, within RR42_RS27995 at 1644.713 kb on - strand, within RR42_RS27995 at 1644.743 kb on + strand, within RR42_RS27995 at 1644.821 kb on + strand at 1644.821 kb on + strand at 1644.822 kb on - strand at 1644.963 kb on + strand, within RR42_RS28000 at 1644.986 kb on - strand, within RR42_RS28000 at 1645.703 kb on + strand, within RR42_RS28005 at 1645.954 kb on - strand, within RR42_RS28005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 1,643,906 - RR42_RS27985 0.74 -1.4 1,644,252 + -0.5 1,644,253 - -0.1 1,644,272 + +0.8 1,644,272 + +0.3 1,644,273 - +0.2 1,644,273 - +0.5 1,644,273 - -0.9 1,644,295 - -1.2 1,644,295 - -1.4 1,644,523 + -0.3 1,644,523 + +0.1 1,644,524 - -1.2 1,644,674 - RR42_RS27995 0.54 -0.1 1,644,674 - RR42_RS27995 0.54 -0.4 1,644,713 - RR42_RS27995 0.67 +0.0 1,644,713 - RR42_RS27995 0.67 +0.8 1,644,743 + RR42_RS27995 0.78 -1.7 1,644,821 + -1.7 1,644,821 + +0.6 1,644,822 - +1.3 1,644,963 + RR42_RS28000 0.54 -1.9 1,644,986 - RR42_RS28000 0.63 -1.2 1,645,703 + RR42_RS28005 0.59 +1.0 1,645,954 - RR42_RS28005 0.89 +2.0
Or see this region's nucleotide sequence