Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27870 and RR42_RS27875 are separated by 33 nucleotides RR42_RS27875 and RR42_RS27880 are separated by 79 nucleotides
RR42_RS27870: RR42_RS27870 - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase, at 1,615,361 to 1,616,245
_RS27870
RR42_RS27875: RR42_RS27875 - 3-(3-hydroxyphenyl)propionate hydroxylase, at 1,616,279 to 1,618,063
_RS27875
RR42_RS27880: RR42_RS27880 - 3-(2,3-dihydroxyphenyl)propionate dioxygenase, at 1,618,143 to 1,619,093
_RS27880
Position (kb) 1616
1617
1618
1619 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1615.445 kb on + strand at 1615.517 kb on + strand, within RR42_RS27870 at 1615.518 kb on - strand, within RR42_RS27870 at 1615.931 kb on + strand, within RR42_RS27870 at 1615.932 kb on - strand, within RR42_RS27870 at 1615.932 kb on - strand, within RR42_RS27870 at 1616.024 kb on + strand, within RR42_RS27870 at 1616.025 kb on - strand, within RR42_RS27870 at 1616.386 kb on - strand at 1616.560 kb on + strand, within RR42_RS27875 at 1616.629 kb on + strand, within RR42_RS27875 at 1616.792 kb on + strand, within RR42_RS27875 at 1616.920 kb on + strand, within RR42_RS27875 at 1616.920 kb on + strand, within RR42_RS27875 at 1616.920 kb on + strand, within RR42_RS27875 at 1616.921 kb on - strand, within RR42_RS27875 at 1616.921 kb on - strand, within RR42_RS27875 at 1616.921 kb on - strand, within RR42_RS27875 at 1616.924 kb on + strand, within RR42_RS27875 at 1616.925 kb on - strand, within RR42_RS27875 at 1616.925 kb on - strand, within RR42_RS27875 at 1616.951 kb on + strand, within RR42_RS27875 at 1616.951 kb on + strand, within RR42_RS27875 at 1616.952 kb on - strand, within RR42_RS27875 at 1617.092 kb on + strand, within RR42_RS27875 at 1617.093 kb on - strand, within RR42_RS27875 at 1617.210 kb on - strand, within RR42_RS27875 at 1617.210 kb on - strand, within RR42_RS27875 at 1617.299 kb on + strand, within RR42_RS27875 at 1617.300 kb on - strand, within RR42_RS27875 at 1617.446 kb on + strand, within RR42_RS27875 at 1617.446 kb on + strand, within RR42_RS27875 at 1617.706 kb on + strand, within RR42_RS27875 at 1617.707 kb on - strand, within RR42_RS27875 at 1617.980 kb on - strand at 1618.094 kb on - strand at 1619.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 1,615,445 + -0.7 1,615,517 + RR42_RS27870 0.18 +0.0 1,615,518 - RR42_RS27870 0.18 -0.7 1,615,931 + RR42_RS27870 0.64 -0.5 1,615,932 - RR42_RS27870 0.65 -0.1 1,615,932 - RR42_RS27870 0.65 +0.6 1,616,024 + RR42_RS27870 0.75 -0.1 1,616,025 - RR42_RS27870 0.75 -0.9 1,616,386 - -0.2 1,616,560 + RR42_RS27875 0.16 -2.1 1,616,629 + RR42_RS27875 0.20 +0.2 1,616,792 + RR42_RS27875 0.29 +0.8 1,616,920 + RR42_RS27875 0.36 +0.5 1,616,920 + RR42_RS27875 0.36 +2.6 1,616,920 + RR42_RS27875 0.36 +1.3 1,616,921 - RR42_RS27875 0.36 -1.0 1,616,921 - RR42_RS27875 0.36 +0.2 1,616,921 - RR42_RS27875 0.36 +0.1 1,616,924 + RR42_RS27875 0.36 -0.6 1,616,925 - RR42_RS27875 0.36 +0.9 1,616,925 - RR42_RS27875 0.36 +0.6 1,616,951 + RR42_RS27875 0.38 -0.8 1,616,951 + RR42_RS27875 0.38 -1.4 1,616,952 - RR42_RS27875 0.38 +1.6 1,617,092 + RR42_RS27875 0.46 -0.8 1,617,093 - RR42_RS27875 0.46 +1.1 1,617,210 - RR42_RS27875 0.52 +0.4 1,617,210 - RR42_RS27875 0.52 -0.4 1,617,299 + RR42_RS27875 0.57 +0.5 1,617,300 - RR42_RS27875 0.57 -0.7 1,617,446 + RR42_RS27875 0.65 +0.6 1,617,446 + RR42_RS27875 0.65 +0.3 1,617,706 + RR42_RS27875 0.80 +0.3 1,617,707 - RR42_RS27875 0.80 -0.4 1,617,980 - +0.5 1,618,094 - +0.0 1,619,002 - +2.0
Or see this region's nucleotide sequence