Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27405 and RR42_RS27410 are separated by 90 nucleotides RR42_RS27410 and RR42_RS27415 are separated by 125 nucleotides
RR42_RS27405: RR42_RS27405 - aminotransferase, at 1,499,158 to 1,500,501
_RS27405
RR42_RS27410: RR42_RS27410 - hydroxyacid dehydrogenase, at 1,500,592 to 1,501,551
_RS27410
RR42_RS27415: RR42_RS27415 - microcin ABC transporter ATP-binding protein, at 1,501,677 to 1,503,272
_RS27415
Position (kb)
1500
1501
1502 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1499.627 kb on + strand, within RR42_RS27405 at 1499.627 kb on + strand, within RR42_RS27405 at 1499.628 kb on - strand, within RR42_RS27405 at 1499.786 kb on + strand, within RR42_RS27405 at 1499.786 kb on + strand, within RR42_RS27405 at 1499.787 kb on - strand, within RR42_RS27405 at 1499.787 kb on - strand, within RR42_RS27405 at 1500.110 kb on + strand, within RR42_RS27405 at 1500.111 kb on - strand, within RR42_RS27405 at 1500.113 kb on + strand, within RR42_RS27405 at 1500.257 kb on + strand, within RR42_RS27405 at 1500.257 kb on + strand, within RR42_RS27405 at 1500.258 kb on - strand, within RR42_RS27405 at 1500.383 kb on + strand at 1500.383 kb on + strand at 1500.383 kb on + strand at 1500.383 kb on + strand at 1500.383 kb on + strand at 1500.383 kb on + strand at 1500.384 kb on - strand at 1500.384 kb on - strand at 1500.384 kb on - strand at 1500.384 kb on - strand at 1500.590 kb on + strand at 1500.590 kb on + strand at 1500.591 kb on - strand at 1500.596 kb on + strand at 1500.856 kb on + strand, within RR42_RS27410 at 1500.857 kb on - strand, within RR42_RS27410 at 1501.188 kb on - strand, within RR42_RS27410 at 1501.232 kb on + strand, within RR42_RS27410 at 1501.233 kb on - strand, within RR42_RS27410 at 1501.233 kb on - strand, within RR42_RS27410 at 1501.233 kb on - strand, within RR42_RS27410 at 1501.714 kb on + strand at 1502.088 kb on + strand, within RR42_RS27415 at 1502.088 kb on + strand, within RR42_RS27415 at 1502.155 kb on + strand, within RR42_RS27415 at 1502.155 kb on + strand, within RR42_RS27415 at 1502.156 kb on - strand, within RR42_RS27415 at 1502.518 kb on + strand, within RR42_RS27415 at 1502.518 kb on + strand, within RR42_RS27415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 1,499,627 + RR42_RS27405 0.35 +1.1 1,499,627 + RR42_RS27405 0.35 +1.0 1,499,628 - RR42_RS27405 0.35 +0.1 1,499,786 + RR42_RS27405 0.47 +1.3 1,499,786 + RR42_RS27405 0.47 +0.5 1,499,787 - RR42_RS27405 0.47 +0.3 1,499,787 - RR42_RS27405 0.47 -0.8 1,500,110 + RR42_RS27405 0.71 +0.2 1,500,111 - RR42_RS27405 0.71 +1.1 1,500,113 + RR42_RS27405 0.71 -0.6 1,500,257 + RR42_RS27405 0.82 -0.0 1,500,257 + RR42_RS27405 0.82 +0.8 1,500,258 - RR42_RS27405 0.82 +0.5 1,500,383 + -1.2 1,500,383 + -0.6 1,500,383 + -0.2 1,500,383 + +0.8 1,500,383 + -1.9 1,500,383 + -0.2 1,500,384 - +0.0 1,500,384 - +0.0 1,500,384 - +2.1 1,500,384 - -0.2 1,500,590 + -0.4 1,500,590 + -0.2 1,500,591 - -0.5 1,500,596 + +0.4 1,500,856 + RR42_RS27410 0.28 -1.0 1,500,857 - RR42_RS27410 0.28 -1.2 1,501,188 - RR42_RS27410 0.62 -0.9 1,501,232 + RR42_RS27410 0.67 -0.4 1,501,233 - RR42_RS27410 0.67 +0.5 1,501,233 - RR42_RS27410 0.67 -0.1 1,501,233 - RR42_RS27410 0.67 -1.4 1,501,714 + +2.1 1,502,088 + RR42_RS27415 0.26 -0.5 1,502,088 + RR42_RS27415 0.26 -0.1 1,502,155 + RR42_RS27415 0.30 +1.2 1,502,155 + RR42_RS27415 0.30 -1.2 1,502,156 - RR42_RS27415 0.30 -0.3 1,502,518 + RR42_RS27415 0.53 +1.5 1,502,518 + RR42_RS27415 0.53 -2.5
Or see this region's nucleotide sequence