Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25180 and RR42_RS25185 are separated by 226 nucleotides RR42_RS25185 and RR42_RS25190 are separated by 171 nucleotides
RR42_RS25180: RR42_RS25180 - AraC family transcriptional regulator, at 989,279 to 990,148
_RS25180
RR42_RS25185: RR42_RS25185 - epimerase, at 990,375 to 991,253
_RS25185
RR42_RS25190: RR42_RS25190 - acyl-CoA dehydrogenase, at 991,425 to 993,194
_RS25190
Position (kb)
990
991
992 Strain fitness (log2 ratio)
-2
-1
0
1 at 989.568 kb on + strand, within RR42_RS25180 at 989.586 kb on - strand, within RR42_RS25180 at 989.694 kb on + strand, within RR42_RS25180 at 989.727 kb on - strand, within RR42_RS25180 at 989.727 kb on - strand, within RR42_RS25180 at 989.856 kb on - strand, within RR42_RS25180 at 989.856 kb on - strand, within RR42_RS25180 at 989.981 kb on + strand, within RR42_RS25180 at 990.173 kb on + strand at 990.174 kb on - strand at 990.245 kb on + strand at 990.306 kb on - strand at 990.340 kb on + strand at 990.388 kb on - strand at 990.584 kb on - strand, within RR42_RS25185 at 990.587 kb on + strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.665 kb on - strand, within RR42_RS25185 at 990.715 kb on - strand, within RR42_RS25185 at 990.845 kb on + strand, within RR42_RS25185 at 990.845 kb on + strand, within RR42_RS25185 at 990.846 kb on - strand, within RR42_RS25185 at 991.184 kb on + strand at 991.239 kb on - strand at 991.260 kb on + strand at 991.261 kb on - strand at 991.273 kb on + strand at 991.273 kb on + strand at 991.273 kb on + strand at 991.274 kb on - strand at 991.274 kb on - strand at 991.359 kb on + strand at 991.360 kb on - strand at 991.489 kb on - strand at 991.489 kb on - strand at 991.513 kb on + strand at 991.513 kb on + strand at 991.543 kb on + strand at 991.544 kb on - strand at 991.544 kb on - strand at 991.544 kb on - strand at 991.609 kb on - strand, within RR42_RS25190 at 991.609 kb on - strand, within RR42_RS25190 at 991.636 kb on + strand, within RR42_RS25190 at 991.636 kb on + strand, within RR42_RS25190 at 991.636 kb on + strand, within RR42_RS25190 at 991.637 kb on - strand, within RR42_RS25190 at 991.637 kb on - strand, within RR42_RS25190 at 991.689 kb on + strand, within RR42_RS25190 at 991.689 kb on + strand, within RR42_RS25190 at 991.689 kb on + strand, within RR42_RS25190 at 991.899 kb on - strand, within RR42_RS25190 at 991.932 kb on - strand, within RR42_RS25190 at 991.941 kb on - strand, within RR42_RS25190 at 991.941 kb on - strand, within RR42_RS25190 at 991.942 kb on + strand, within RR42_RS25190 at 991.943 kb on - strand, within RR42_RS25190 at 991.943 kb on - strand, within RR42_RS25190 at 991.943 kb on - strand, within RR42_RS25190 at 991.943 kb on - strand, within RR42_RS25190 at 992.012 kb on + strand, within RR42_RS25190 at 992.137 kb on + strand, within RR42_RS25190 at 992.137 kb on + strand, within RR42_RS25190 at 992.137 kb on + strand, within RR42_RS25190 at 992.137 kb on + strand, within RR42_RS25190 at 992.138 kb on - strand, within RR42_RS25190 at 992.138 kb on - strand, within RR42_RS25190 at 992.159 kb on + strand, within RR42_RS25190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 989,568 + RR42_RS25180 0.33 -0.6 989,586 - RR42_RS25180 0.35 +0.9 989,694 + RR42_RS25180 0.48 +0.8 989,727 - RR42_RS25180 0.51 -0.6 989,727 - RR42_RS25180 0.51 -0.1 989,856 - RR42_RS25180 0.66 -0.6 989,856 - RR42_RS25180 0.66 +0.5 989,981 + RR42_RS25180 0.81 -1.2 990,173 + -1.2 990,174 - -0.2 990,245 + -0.0 990,306 - +1.5 990,340 + -0.0 990,388 - -0.0 990,584 - RR42_RS25185 0.24 -1.7 990,587 + RR42_RS25185 0.24 -0.8 990,665 - RR42_RS25185 0.33 -0.8 990,665 - RR42_RS25185 0.33 -1.4 990,665 - RR42_RS25185 0.33 +0.7 990,715 - RR42_RS25185 0.39 -0.4 990,845 + RR42_RS25185 0.53 -0.3 990,845 + RR42_RS25185 0.53 +0.9 990,846 - RR42_RS25185 0.54 -0.7 991,184 + -0.7 991,239 - +0.1 991,260 + -0.5 991,261 - +0.3 991,273 + +0.9 991,273 + -0.0 991,273 + +1.2 991,274 - -0.2 991,274 - +0.3 991,359 + -0.6 991,360 - -2.4 991,489 - +0.0 991,489 - +1.6 991,513 + +0.6 991,513 + -1.7 991,543 + +0.7 991,544 - -1.2 991,544 - +0.1 991,544 - +1.1 991,609 - RR42_RS25190 0.10 -0.6 991,609 - RR42_RS25190 0.10 +1.0 991,636 + RR42_RS25190 0.12 +1.3 991,636 + RR42_RS25190 0.12 +0.2 991,636 + RR42_RS25190 0.12 -1.3 991,637 - RR42_RS25190 0.12 -0.0 991,637 - RR42_RS25190 0.12 +0.3 991,689 + RR42_RS25190 0.15 +0.8 991,689 + RR42_RS25190 0.15 -0.8 991,689 + RR42_RS25190 0.15 +0.5 991,899 - RR42_RS25190 0.27 +0.1 991,932 - RR42_RS25190 0.29 +0.8 991,941 - RR42_RS25190 0.29 -0.5 991,941 - RR42_RS25190 0.29 +1.3 991,942 + RR42_RS25190 0.29 -0.6 991,943 - RR42_RS25190 0.29 +1.0 991,943 - RR42_RS25190 0.29 -0.7 991,943 - RR42_RS25190 0.29 +0.3 991,943 - RR42_RS25190 0.29 +0.7 992,012 + RR42_RS25190 0.33 +0.9 992,137 + RR42_RS25190 0.40 +0.7 992,137 + RR42_RS25190 0.40 +0.6 992,137 + RR42_RS25190 0.40 +0.3 992,137 + RR42_RS25190 0.40 +1.2 992,138 - RR42_RS25190 0.40 +1.3 992,138 - RR42_RS25190 0.40 -0.1 992,159 + RR42_RS25190 0.41 +0.1
Or see this region's nucleotide sequence