Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23730 and RR42_RS23735 are separated by 15 nucleotides RR42_RS23735 and RR42_RS23740 are separated by 6 nucleotides RR42_RS23740 and RR42_RS23745 are separated by 26 nucleotides
RR42_RS23730: RR42_RS23730 - phenylacetate-CoA oxygenase subunit PaaB, at 649,575 to 649,859
_RS23730
RR42_RS23735: RR42_RS23735 - phenylacetyl-CoA 1,2-epoxidase, structural subunit (EC 1.14.13.149) (from data) , at 649,875 to 650,675
_RS23735
RR42_RS23740: RR42_RS23740 - phenylacetate-CoA oxygenase, at 650,682 to 651,248
_RS23740
RR42_RS23745: RR42_RS23745 - phenylacetic acid degradation protein, at 651,275 to 652,363
_RS23745
Position (kb)
650
651
652 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 649.698 kb on + strand, within RR42_RS23730 at 649.699 kb on - strand, within RR42_RS23730 at 649.699 kb on - strand, within RR42_RS23730 at 649.857 kb on + strand at 649.857 kb on + strand at 649.857 kb on + strand at 649.858 kb on - strand at 649.858 kb on - strand at 649.928 kb on + strand at 649.987 kb on + strand, within RR42_RS23735 at 649.987 kb on + strand, within RR42_RS23735 at 650.038 kb on - strand, within RR42_RS23735 at 650.038 kb on - strand, within RR42_RS23735 at 650.097 kb on + strand, within RR42_RS23735 at 650.097 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.134 kb on - strand, within RR42_RS23735 at 650.134 kb on - strand, within RR42_RS23735 at 650.196 kb on + strand, within RR42_RS23735 at 650.197 kb on - strand, within RR42_RS23735 at 650.197 kb on - strand, within RR42_RS23735 at 650.226 kb on + strand, within RR42_RS23735 at 650.226 kb on + strand, within RR42_RS23735 at 650.410 kb on - strand, within RR42_RS23735 at 650.620 kb on - strand at 650.673 kb on + strand at 650.856 kb on + strand, within RR42_RS23740 at 651.198 kb on + strand at 651.199 kb on - strand at 651.199 kb on - strand at 651.231 kb on + strand at 651.244 kb on - strand at 651.636 kb on - strand, within RR42_RS23745 at 652.136 kb on + strand, within RR42_RS23745 at 652.137 kb on - strand, within RR42_RS23745 at 652.190 kb on + strand, within RR42_RS23745 at 652.190 kb on + strand, within RR42_RS23745 at 652.191 kb on - strand, within RR42_RS23745 at 652.191 kb on - strand, within RR42_RS23745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 649,698 + RR42_RS23730 0.43 +0.1 649,699 - RR42_RS23730 0.44 +2.1 649,699 - RR42_RS23730 0.44 +2.8 649,857 + -1.4 649,857 + +0.3 649,857 + +0.1 649,858 - +0.2 649,858 - +0.1 649,928 + +0.2 649,987 + RR42_RS23735 0.14 +1.8 649,987 + RR42_RS23735 0.14 -1.0 650,038 - RR42_RS23735 0.20 -1.3 650,038 - RR42_RS23735 0.20 +0.4 650,097 + RR42_RS23735 0.28 -0.3 650,097 + RR42_RS23735 0.28 -1.0 650,133 + RR42_RS23735 0.32 +1.1 650,133 + RR42_RS23735 0.32 +1.5 650,133 + RR42_RS23735 0.32 -0.4 650,133 + RR42_RS23735 0.32 +0.8 650,134 - RR42_RS23735 0.32 -0.0 650,134 - RR42_RS23735 0.32 -0.6 650,196 + RR42_RS23735 0.40 -0.8 650,197 - RR42_RS23735 0.40 -1.8 650,197 - RR42_RS23735 0.40 +0.1 650,226 + RR42_RS23735 0.44 +0.4 650,226 + RR42_RS23735 0.44 -0.1 650,410 - RR42_RS23735 0.67 -0.4 650,620 - +2.0 650,673 + -0.8 650,856 + RR42_RS23740 0.31 +2.0 651,198 + +1.4 651,199 - +0.6 651,199 - -0.0 651,231 + +0.2 651,244 - -0.0 651,636 - RR42_RS23745 0.33 +0.1 652,136 + RR42_RS23745 0.79 -0.8 652,137 - RR42_RS23745 0.79 -1.4 652,190 + RR42_RS23745 0.84 -0.5 652,190 + RR42_RS23745 0.84 -0.1 652,191 - RR42_RS23745 0.84 -1.6 652,191 - RR42_RS23745 0.84 -1.6
Or see this region's nucleotide sequence