Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23480 and RR42_RS23485 are separated by 152 nucleotides RR42_RS23485 and RR42_RS23490 are separated by 28 nucleotides RR42_RS23490 and RR42_RS23495 are separated by 25 nucleotides RR42_RS23495 and RR42_RS23500 are separated by 59 nucleotides
RR42_RS23480: RR42_RS23480 - 3-keto-5-aminohexanoate cleavage protein, at 594,357 to 595,190
_RS23480
RR42_RS23485: RR42_RS23485 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 595,343 to 596,128
_RS23485
RR42_RS23490: RR42_RS23490 - NADH oxidase, at 596,157 to 597,176
_RS23490
RR42_RS23495: RR42_RS23495 - benzene 1,2-dioxygenase, at 597,202 to 597,684
_RS23495
RR42_RS23500: RR42_RS23500 - benzene 1,2-dioxygenase, at 597,744 to 599,132
_RS23500
Position (kb) 596
597
598 Strain fitness (log2 ratio) -2
-1
0
1
2 at 595.173 kb on - strand at 595.195 kb on - strand at 595.214 kb on + strand at 595.214 kb on + strand at 595.215 kb on - strand at 595.217 kb on - strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.390 kb on - strand at 595.390 kb on - strand at 595.497 kb on + strand, within RR42_RS23485 at 595.674 kb on + strand, within RR42_RS23485 at 595.675 kb on - strand, within RR42_RS23485 at 595.675 kb on - strand, within RR42_RS23485 at 596.128 kb on + strand at 596.129 kb on - strand at 596.269 kb on + strand, within RR42_RS23490 at 596.269 kb on + strand, within RR42_RS23490 at 596.270 kb on - strand, within RR42_RS23490 at 596.270 kb on - strand, within RR42_RS23490 at 596.270 kb on - strand, within RR42_RS23490 at 596.324 kb on - strand, within RR42_RS23490 at 596.449 kb on + strand, within RR42_RS23490 at 596.449 kb on + strand, within RR42_RS23490 at 596.450 kb on - strand, within RR42_RS23490 at 596.450 kb on - strand, within RR42_RS23490 at 596.664 kb on + strand, within RR42_RS23490 at 596.665 kb on - strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.753 kb on - strand, within RR42_RS23490 at 596.984 kb on + strand, within RR42_RS23490 at 596.985 kb on - strand, within RR42_RS23490 at 596.986 kb on + strand, within RR42_RS23490 at 596.987 kb on - strand, within RR42_RS23490 at 597.169 kb on + strand at 597.169 kb on + strand at 597.169 kb on + strand at 597.169 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.213 kb on - strand at 597.213 kb on - strand at 597.213 kb on - strand at 597.236 kb on + strand at 597.257 kb on + strand, within RR42_RS23495 at 597.303 kb on - strand, within RR42_RS23495 at 597.326 kb on + strand, within RR42_RS23495 at 597.327 kb on - strand, within RR42_RS23495 at 597.327 kb on - strand, within RR42_RS23495 at 597.599 kb on + strand, within RR42_RS23495 at 597.818 kb on - strand at 598.045 kb on + strand, within RR42_RS23500 at 598.102 kb on + strand, within RR42_RS23500 at 598.102 kb on + strand, within RR42_RS23500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 595,173 - +0.3 595,195 - +2.4 595,214 + -0.3 595,214 + +0.7 595,215 - -1.4 595,217 - +2.0 595,389 + -0.9 595,389 + -0.2 595,389 + -0.1 595,389 + +0.6 595,389 + +0.4 595,389 + -0.4 595,390 - -0.8 595,390 - +0.5 595,497 + RR42_RS23485 0.20 -0.8 595,674 + RR42_RS23485 0.42 -2.1 595,675 - RR42_RS23485 0.42 -0.6 595,675 - RR42_RS23485 0.42 -0.3 596,128 + +1.2 596,129 - +0.6 596,269 + RR42_RS23490 0.11 -0.6 596,269 + RR42_RS23490 0.11 -0.3 596,270 - RR42_RS23490 0.11 -0.1 596,270 - RR42_RS23490 0.11 +1.6 596,270 - RR42_RS23490 0.11 -0.2 596,324 - RR42_RS23490 0.16 +0.9 596,449 + RR42_RS23490 0.29 -0.0 596,449 + RR42_RS23490 0.29 +1.1 596,450 - RR42_RS23490 0.29 +0.6 596,450 - RR42_RS23490 0.29 +0.4 596,664 + RR42_RS23490 0.50 -1.1 596,665 - RR42_RS23490 0.50 -1.0 596,752 + RR42_RS23490 0.58 -0.0 596,752 + RR42_RS23490 0.58 -1.0 596,752 + RR42_RS23490 0.58 +2.1 596,752 + RR42_RS23490 0.58 +0.5 596,752 + RR42_RS23490 0.58 +1.4 596,753 - RR42_RS23490 0.58 +1.4 596,984 + RR42_RS23490 0.81 -0.5 596,985 - RR42_RS23490 0.81 -0.3 596,986 + RR42_RS23490 0.81 -0.9 596,987 - RR42_RS23490 0.81 -1.0 597,169 + +0.3 597,169 + -0.2 597,169 + -0.1 597,169 + -0.7 597,212 + +0.0 597,212 + -1.2 597,212 + +1.2 597,212 + +1.1 597,213 - +0.2 597,213 - +0.6 597,213 - -0.9 597,236 + -0.2 597,257 + RR42_RS23495 0.11 +0.5 597,303 - RR42_RS23495 0.21 -0.5 597,326 + RR42_RS23495 0.26 -0.6 597,327 - RR42_RS23495 0.26 +0.9 597,327 - RR42_RS23495 0.26 +0.9 597,599 + RR42_RS23495 0.82 +1.4 597,818 - +0.7 598,045 + RR42_RS23500 0.22 -1.0 598,102 + RR42_RS23500 0.26 +0.9 598,102 + RR42_RS23500 0.26 +1.4
Or see this region's nucleotide sequence