Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22950 and RR42_RS22955 are separated by 126 nucleotides RR42_RS22955 and RR42_RS22960 are separated by 168 nucleotides
RR42_RS22950: RR42_RS22950 - cytochrome C550, at 482,692 to 483,549
_RS22950
RR42_RS22955: RR42_RS22955 - isocitrate dehydrogenase, at 483,676 to 484,707
_RS22955
RR42_RS22960: RR42_RS22960 - alpha/beta hydrolase, at 484,876 to 485,757
_RS22960
Position (kb) 483
484
485 Strain fitness (log2 ratio) -2
-1
0
1
2 at 482.679 kb on + strand at 482.680 kb on - strand at 482.710 kb on + strand at 482.772 kb on + strand at 482.773 kb on - strand at 482.773 kb on - strand at 482.791 kb on + strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.963 kb on - strand, within RR42_RS22950 at 482.963 kb on - strand, within RR42_RS22950 at 483.352 kb on - strand, within RR42_RS22950 at 483.352 kb on - strand, within RR42_RS22950 at 483.977 kb on + strand, within RR42_RS22955 at 483.977 kb on + strand, within RR42_RS22955 at 483.977 kb on + strand, within RR42_RS22955 at 484.017 kb on + strand, within RR42_RS22955 at 484.057 kb on + strand, within RR42_RS22955 at 484.058 kb on - strand, within RR42_RS22955 at 484.073 kb on - strand, within RR42_RS22955 at 484.073 kb on - strand, within RR42_RS22955 at 484.075 kb on + strand, within RR42_RS22955 at 484.076 kb on - strand, within RR42_RS22955 at 484.128 kb on + strand, within RR42_RS22955 at 484.171 kb on + strand, within RR42_RS22955 at 484.171 kb on + strand, within RR42_RS22955 at 484.280 kb on - strand, within RR42_RS22955 at 484.371 kb on + strand, within RR42_RS22955 at 484.895 kb on - strand at 484.895 kb on - strand at 485.054 kb on - strand, within RR42_RS22960 at 485.062 kb on + strand, within RR42_RS22960 at 485.062 kb on + strand, within RR42_RS22960 at 485.251 kb on + strand, within RR42_RS22960 at 485.252 kb on - strand, within RR42_RS22960 at 485.252 kb on - strand, within RR42_RS22960 at 485.285 kb on + strand, within RR42_RS22960 at 485.286 kb on - strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.428 kb on + strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.429 kb on - strand, within RR42_RS22960 at 485.517 kb on + strand, within RR42_RS22960 at 485.518 kb on - strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 482,679 + -0.3 482,680 - -0.3 482,710 + +1.5 482,772 + -0.8 482,773 - +0.1 482,773 - -0.3 482,791 + RR42_RS22950 0.12 +0.6 482,792 - RR42_RS22950 0.12 +1.3 482,792 - RR42_RS22950 0.12 +0.2 482,792 - RR42_RS22950 0.12 +0.9 482,809 + RR42_RS22950 0.14 -1.0 482,809 + RR42_RS22950 0.14 +0.7 482,809 + RR42_RS22950 0.14 +1.9 482,962 + RR42_RS22950 0.31 -0.6 482,962 + RR42_RS22950 0.31 -1.3 482,962 + RR42_RS22950 0.31 +1.7 482,962 + RR42_RS22950 0.31 +0.5 482,963 - RR42_RS22950 0.32 -0.8 482,963 - RR42_RS22950 0.32 -1.1 483,352 - RR42_RS22950 0.77 +0.5 483,352 - RR42_RS22950 0.77 -1.5 483,977 + RR42_RS22955 0.29 -1.6 483,977 + RR42_RS22955 0.29 +0.2 483,977 + RR42_RS22955 0.29 -2.3 484,017 + RR42_RS22955 0.33 -0.1 484,057 + RR42_RS22955 0.37 -0.3 484,058 - RR42_RS22955 0.37 +0.0 484,073 - RR42_RS22955 0.38 -0.5 484,073 - RR42_RS22955 0.38 +0.1 484,075 + RR42_RS22955 0.39 -1.5 484,076 - RR42_RS22955 0.39 -0.8 484,128 + RR42_RS22955 0.44 -0.8 484,171 + RR42_RS22955 0.48 -2.0 484,171 + RR42_RS22955 0.48 +1.5 484,280 - RR42_RS22955 0.59 +1.5 484,371 + RR42_RS22955 0.67 +0.6 484,895 - -1.1 484,895 - -0.5 485,054 - RR42_RS22960 0.20 -1.1 485,062 + RR42_RS22960 0.21 +1.5 485,062 + RR42_RS22960 0.21 -1.5 485,251 + RR42_RS22960 0.43 -0.5 485,252 - RR42_RS22960 0.43 -0.4 485,252 - RR42_RS22960 0.43 -0.2 485,285 + RR42_RS22960 0.46 -0.6 485,286 - RR42_RS22960 0.46 +0.9 485,428 + RR42_RS22960 0.63 -0.3 485,428 + RR42_RS22960 0.63 -0.5 485,428 + RR42_RS22960 0.63 +0.3 485,428 + RR42_RS22960 0.63 +0.9 485,428 + RR42_RS22960 0.63 +1.7 485,429 - RR42_RS22960 0.63 -1.2 485,429 - RR42_RS22960 0.63 -1.6 485,429 - RR42_RS22960 0.63 +1.1 485,429 - RR42_RS22960 0.63 +1.7 485,429 - RR42_RS22960 0.63 -0.1 485,517 + RR42_RS22960 0.73 -0.2 485,518 - RR42_RS22960 0.73 +2.0 485,551 + RR42_RS22960 0.77 +0.0 485,551 + RR42_RS22960 0.77 -0.7 485,551 + RR42_RS22960 0.77 +0.5 485,551 + RR42_RS22960 0.77 +0.4 485,552 - RR42_RS22960 0.77 +1.5 485,552 - RR42_RS22960 0.77 -0.7 485,552 - RR42_RS22960 0.77 +0.0
Or see this region's nucleotide sequence