Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22710 and RR42_RS22715 are separated by 225 nucleotides RR42_RS22715 and RR42_RS22720 overlap by 17 nucleotides
RR42_RS22710: RR42_RS22710 - short-chain dehydrogenase, at 428,992 to 429,741
_RS22710
RR42_RS22715: RR42_RS22715 - AMP-binding protein, at 429,967 to 431,616
_RS22715
RR42_RS22720: RR42_RS22720 - hypothetical protein, at 431,600 to 431,977
_RS22720
Position (kb)
429
430
431
432 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 428.967 kb on - strand at 428.967 kb on - strand at 429.180 kb on - strand, within RR42_RS22710 at 429.293 kb on + strand, within RR42_RS22710 at 429.782 kb on + strand at 429.782 kb on + strand at 429.782 kb on + strand at 429.801 kb on + strand at 429.968 kb on + strand at 429.969 kb on - strand at 430.080 kb on - strand at 430.148 kb on + strand, within RR42_RS22715 at 430.317 kb on + strand, within RR42_RS22715 at 430.367 kb on + strand, within RR42_RS22715 at 430.367 kb on + strand, within RR42_RS22715 at 430.368 kb on - strand, within RR42_RS22715 at 430.368 kb on - strand, within RR42_RS22715 at 430.370 kb on + strand, within RR42_RS22715 at 430.371 kb on - strand, within RR42_RS22715 at 430.441 kb on - strand, within RR42_RS22715 at 430.604 kb on + strand, within RR42_RS22715 at 430.832 kb on + strand, within RR42_RS22715 at 430.877 kb on + strand, within RR42_RS22715 at 430.877 kb on + strand, within RR42_RS22715 at 430.878 kb on - strand, within RR42_RS22715 at 430.880 kb on + strand, within RR42_RS22715 at 430.880 kb on + strand, within RR42_RS22715 at 430.880 kb on + strand, within RR42_RS22715 at 430.880 kb on + strand, within RR42_RS22715 at 430.881 kb on - strand, within RR42_RS22715 at 430.881 kb on - strand, within RR42_RS22715 at 430.889 kb on + strand, within RR42_RS22715 at 430.890 kb on - strand, within RR42_RS22715 at 430.956 kb on + strand, within RR42_RS22715 at 430.956 kb on + strand, within RR42_RS22715 at 430.956 kb on + strand, within RR42_RS22715 at 430.957 kb on - strand, within RR42_RS22715 at 431.161 kb on + strand, within RR42_RS22715 at 431.161 kb on + strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.162 kb on - strand, within RR42_RS22715 at 431.179 kb on + strand, within RR42_RS22715 at 431.179 kb on + strand, within RR42_RS22715 at 431.179 kb on + strand, within RR42_RS22715 at 431.179 kb on + strand, within RR42_RS22715 at 431.180 kb on - strand, within RR42_RS22715 at 431.180 kb on - strand, within RR42_RS22715 at 431.442 kb on + strand, within RR42_RS22715 at 431.442 kb on + strand, within RR42_RS22715 at 431.442 kb on + strand, within RR42_RS22715 at 431.443 kb on - strand, within RR42_RS22715 at 431.443 kb on - strand, within RR42_RS22715 at 431.443 kb on - strand, within RR42_RS22715 at 431.443 kb on - strand, within RR42_RS22715 at 431.444 kb on + strand, within RR42_RS22715 at 431.444 kb on + strand, within RR42_RS22715 at 431.445 kb on - strand, within RR42_RS22715 at 431.637 kb on + strand at 431.638 kb on - strand, within RR42_RS22720 at 432.013 kb on + strand at 432.031 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.034 kb on + strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.035 kb on - strand at 432.046 kb on + strand at 432.095 kb on - strand at 432.095 kb on - strand at 432.125 kb on - strand at 432.125 kb on - strand at 432.148 kb on + strand at 432.148 kb on + strand at 432.149 kb on - strand at 432.193 kb on + strand at 432.194 kb on - strand at 432.300 kb on - strand at 432.494 kb on + strand at 432.495 kb on - strand at 432.495 kb on - strand at 432.536 kb on + strand at 432.536 kb on + strand at 432.613 kb on + strand at 432.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 428,967 - -1.8 428,967 - -0.8 429,180 - RR42_RS22710 0.25 +0.7 429,293 + RR42_RS22710 0.40 -0.9 429,782 + +2.3 429,782 + +2.8 429,782 + +1.5 429,801 + -0.8 429,968 + -1.2 429,969 - +0.6 430,080 - -0.4 430,148 + RR42_RS22715 0.11 +1.3 430,317 + RR42_RS22715 0.21 -0.8 430,367 + RR42_RS22715 0.24 +0.8 430,367 + RR42_RS22715 0.24 -0.5 430,368 - RR42_RS22715 0.24 +1.2 430,368 - RR42_RS22715 0.24 -0.3 430,370 + RR42_RS22715 0.24 +0.9 430,371 - RR42_RS22715 0.24 +0.2 430,441 - RR42_RS22715 0.29 -0.8 430,604 + RR42_RS22715 0.39 +0.4 430,832 + RR42_RS22715 0.52 +0.5 430,877 + RR42_RS22715 0.55 +0.9 430,877 + RR42_RS22715 0.55 +0.6 430,878 - RR42_RS22715 0.55 +1.9 430,880 + RR42_RS22715 0.55 +1.6 430,880 + RR42_RS22715 0.55 +0.2 430,880 + RR42_RS22715 0.55 +0.6 430,880 + RR42_RS22715 0.55 +0.0 430,881 - RR42_RS22715 0.55 -0.5 430,881 - RR42_RS22715 0.55 -1.2 430,889 + RR42_RS22715 0.56 -0.6 430,890 - RR42_RS22715 0.56 -0.1 430,956 + RR42_RS22715 0.60 +0.0 430,956 + RR42_RS22715 0.60 -0.0 430,956 + RR42_RS22715 0.60 +0.2 430,957 - RR42_RS22715 0.60 +0.5 431,161 + RR42_RS22715 0.72 +0.9 431,161 + RR42_RS22715 0.72 +0.5 431,162 - RR42_RS22715 0.72 +0.1 431,162 - RR42_RS22715 0.72 +0.0 431,162 - RR42_RS22715 0.72 -0.6 431,162 - RR42_RS22715 0.72 -0.1 431,162 - RR42_RS22715 0.72 -0.6 431,162 - RR42_RS22715 0.72 +0.2 431,179 + RR42_RS22715 0.73 -0.0 431,179 + RR42_RS22715 0.73 -0.2 431,179 + RR42_RS22715 0.73 -1.1 431,179 + RR42_RS22715 0.73 -0.7 431,180 - RR42_RS22715 0.74 -1.4 431,180 - RR42_RS22715 0.74 +0.9 431,442 + RR42_RS22715 0.89 +0.7 431,442 + RR42_RS22715 0.89 +1.1 431,442 + RR42_RS22715 0.89 -0.3 431,443 - RR42_RS22715 0.89 -1.6 431,443 - RR42_RS22715 0.89 -0.4 431,443 - RR42_RS22715 0.89 +1.0 431,443 - RR42_RS22715 0.89 -0.0 431,444 + RR42_RS22715 0.90 -0.1 431,444 + RR42_RS22715 0.90 +1.8 431,445 - RR42_RS22715 0.90 -0.5 431,637 + +0.8 431,638 - RR42_RS22720 0.10 +0.9 432,013 + +1.8 432,031 + -0.7 432,034 + -0.8 432,034 + +0.3 432,034 + -0.7 432,034 + +1.5 432,034 + +0.8 432,034 + -0.1 432,034 + +2.5 432,034 + +0.1 432,035 - -1.7 432,035 - -0.3 432,035 - -0.7 432,035 - +0.0 432,035 - -0.5 432,046 + -0.6 432,095 - -0.9 432,095 - +2.0 432,125 - +2.1 432,125 - -1.9 432,148 + -0.7 432,148 + -2.9 432,149 - +0.1 432,193 + -0.2 432,194 - +1.7 432,300 - -0.3 432,494 + +0.3 432,495 - -0.2 432,495 - -1.3 432,536 + -1.4 432,536 + +0.9 432,613 + -0.5 432,614 - -0.1
Or see this region's nucleotide sequence