Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21610 and RR42_RS21615 are separated by 414 nucleotides RR42_RS21615 and RR42_RS21620 are separated by 20 nucleotides RR42_RS21620 and RR42_RS21625 are separated by 83 nucleotides
RR42_RS21610: RR42_RS21610 - MFS transporter permease, at 175,831 to 177,141
_RS21610
RR42_RS21615: RR42_RS21615 - alcohol dehydrogenase, at 177,556 to 180,558
_RS21615
RR42_RS21620: RR42_RS21620 - ferredoxin, at 180,579 to 181,085
_RS21620
RR42_RS21625: RR42_RS21625 - 6-hydroxynicotinate 3-monooxygenase, at 181,169 to 182,323
_RS21625
Position (kb) 177
178
179
180
181 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 176.715 kb on - strand, within RR42_RS21610 at 176.715 kb on - strand, within RR42_RS21610 at 176.715 kb on - strand, within RR42_RS21610 at 176.900 kb on - strand, within RR42_RS21610 at 176.916 kb on - strand, within RR42_RS21610 at 176.948 kb on + strand, within RR42_RS21610 at 176.949 kb on - strand, within RR42_RS21610 at 177.032 kb on + strand at 177.033 kb on - strand at 177.077 kb on + strand at 177.078 kb on - strand at 177.104 kb on - strand at 177.106 kb on + strand at 177.129 kb on - strand at 177.134 kb on + strand at 177.134 kb on + strand at 177.170 kb on + strand at 177.272 kb on - strand at 177.466 kb on + strand at 177.558 kb on - strand at 177.615 kb on + strand at 177.615 kb on + strand at 177.724 kb on - strand at 177.724 kb on - strand at 177.726 kb on - strand at 177.890 kb on + strand, within RR42_RS21615 at 178.010 kb on + strand, within RR42_RS21615 at 178.010 kb on + strand, within RR42_RS21615 at 178.011 kb on - strand, within RR42_RS21615 at 178.011 kb on - strand, within RR42_RS21615 at 178.217 kb on + strand, within RR42_RS21615 at 178.400 kb on + strand, within RR42_RS21615 at 178.401 kb on - strand, within RR42_RS21615 at 178.460 kb on + strand, within RR42_RS21615 at 178.466 kb on + strand, within RR42_RS21615 at 178.511 kb on + strand, within RR42_RS21615 at 178.511 kb on - strand, within RR42_RS21615 at 178.526 kb on + strand, within RR42_RS21615 at 178.634 kb on + strand, within RR42_RS21615 at 178.729 kb on + strand, within RR42_RS21615 at 178.730 kb on - strand, within RR42_RS21615 at 179.128 kb on + strand, within RR42_RS21615 at 179.390 kb on + strand, within RR42_RS21615 at 179.391 kb on - strand, within RR42_RS21615 at 179.413 kb on + strand, within RR42_RS21615 at 179.414 kb on - strand, within RR42_RS21615 at 179.732 kb on - strand, within RR42_RS21615 at 179.824 kb on - strand, within RR42_RS21615 at 179.824 kb on - strand, within RR42_RS21615 at 179.834 kb on + strand, within RR42_RS21615 at 179.835 kb on - strand, within RR42_RS21615 at 179.991 kb on - strand, within RR42_RS21615 at 180.036 kb on + strand, within RR42_RS21615 at 180.036 kb on + strand, within RR42_RS21615 at 180.037 kb on - strand, within RR42_RS21615 at 180.188 kb on + strand, within RR42_RS21615 at 180.388 kb on + strand at 180.388 kb on + strand at 180.388 kb on + strand at 180.388 kb on + strand at 180.389 kb on - strand at 180.797 kb on + strand, within RR42_RS21620 at 180.798 kb on - strand, within RR42_RS21620 at 180.958 kb on + strand, within RR42_RS21620 at 180.958 kb on + strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 180.998 kb on - strand, within RR42_RS21620 at 181.039 kb on + strand at 181.040 kb on - strand at 181.040 kb on - strand at 181.040 kb on - strand at 181.273 kb on + strand at 181.274 kb on - strand at 181.424 kb on - strand, within RR42_RS21625 at 181.424 kb on - strand, within RR42_RS21625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 176,715 - RR42_RS21610 0.67 +2.0 176,715 - RR42_RS21610 0.67 -0.4 176,715 - RR42_RS21610 0.67 -0.4 176,900 - RR42_RS21610 0.82 -0.1 176,916 - RR42_RS21610 0.83 -0.5 176,948 + RR42_RS21610 0.85 -0.7 176,949 - RR42_RS21610 0.85 +0.1 177,032 + +1.9 177,033 - -1.1 177,077 + +0.6 177,078 - +0.7 177,104 - -0.9 177,106 + -1.1 177,129 - +0.4 177,134 + -1.5 177,134 + -1.7 177,170 + +0.4 177,272 - +0.2 177,466 + +0.2 177,558 - -0.3 177,615 + +0.3 177,615 + +2.6 177,724 - +0.6 177,724 - +1.4 177,726 - +0.2 177,890 + RR42_RS21615 0.11 -0.5 178,010 + RR42_RS21615 0.15 +1.2 178,010 + RR42_RS21615 0.15 +0.4 178,011 - RR42_RS21615 0.15 -1.2 178,011 - RR42_RS21615 0.15 -0.4 178,217 + RR42_RS21615 0.22 -1.0 178,400 + RR42_RS21615 0.28 +0.4 178,401 - RR42_RS21615 0.28 +0.1 178,460 + RR42_RS21615 0.30 +0.3 178,466 + RR42_RS21615 0.30 -0.2 178,511 + RR42_RS21615 0.32 -0.2 178,511 - RR42_RS21615 0.32 +0.4 178,526 + RR42_RS21615 0.32 +1.9 178,634 + RR42_RS21615 0.36 +1.9 178,729 + RR42_RS21615 0.39 +0.0 178,730 - RR42_RS21615 0.39 +1.4 179,128 + RR42_RS21615 0.52 +0.5 179,390 + RR42_RS21615 0.61 -0.4 179,391 - RR42_RS21615 0.61 +0.3 179,413 + RR42_RS21615 0.62 +2.3 179,414 - RR42_RS21615 0.62 +0.7 179,732 - RR42_RS21615 0.72 -0.4 179,824 - RR42_RS21615 0.76 -1.2 179,824 - RR42_RS21615 0.76 +1.2 179,834 + RR42_RS21615 0.76 -1.7 179,835 - RR42_RS21615 0.76 +1.9 179,991 - RR42_RS21615 0.81 +3.2 180,036 + RR42_RS21615 0.83 +2.6 180,036 + RR42_RS21615 0.83 +1.8 180,037 - RR42_RS21615 0.83 -2.1 180,188 + RR42_RS21615 0.88 +1.4 180,388 + -0.1 180,388 + +0.8 180,388 + -0.7 180,388 + +0.1 180,389 - +0.5 180,797 + RR42_RS21620 0.43 +0.6 180,798 - RR42_RS21620 0.43 +0.8 180,958 + RR42_RS21620 0.75 +1.8 180,958 + RR42_RS21620 0.75 +0.1 180,998 - RR42_RS21620 0.83 +1.0 180,998 - RR42_RS21620 0.83 +0.5 180,998 - RR42_RS21620 0.83 -0.4 181,039 + +0.1 181,040 - -1.2 181,040 - -0.2 181,040 - -1.5 181,273 + +0.4 181,274 - +0.0 181,424 - RR42_RS21625 0.22 +0.9 181,424 - RR42_RS21625 0.22 +0.1
Or see this region's nucleotide sequence