Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18595 and RR42_RS18600 are separated by 89 nucleotides RR42_RS18600 and RR42_RS18605 overlap by 4 nucleotides RR42_RS18605 and RR42_RS18610 are separated by 129 nucleotides RR42_RS18610 and RR42_RS18615 are separated by 59 nucleotides
RR42_RS18595: RR42_RS18595 - ABC transporter substrate-binding protein, at 4,026,350 to 4,027,672
_RS18595
RR42_RS18600: RR42_RS18600 - ABC transporter permease, at 4,027,762 to 4,028,661
_RS18600
RR42_RS18605: RR42_RS18605 - sugar ABC transporter permease, at 4,028,658 to 4,029,545
_RS18605
RR42_RS18610: RR42_RS18610 - hypothetical protein, at 4,029,675 to 4,029,899
_RS18610
RR42_RS18615: RR42_RS18615 - metal-dependent hydrolase, at 4,029,959 to 4,030,453
_RS18615
Position (kb)
4028
4029
4030 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4027.661 kb on + strand at 4027.662 kb on - strand at 4027.662 kb on - strand at 4027.662 kb on - strand at 4027.662 kb on - strand at 4027.662 kb on - strand at 4027.662 kb on - strand at 4027.850 kb on - strand at 4027.930 kb on + strand, within RR42_RS18600 at 4027.930 kb on + strand, within RR42_RS18600 at 4027.937 kb on - strand, within RR42_RS18600 at 4027.977 kb on + strand, within RR42_RS18600 at 4027.987 kb on + strand, within RR42_RS18600 at 4028.021 kb on + strand, within RR42_RS18600 at 4028.143 kb on + strand, within RR42_RS18600 at 4028.143 kb on + strand, within RR42_RS18600 at 4028.144 kb on - strand, within RR42_RS18600 at 4028.144 kb on - strand, within RR42_RS18600 at 4028.186 kb on + strand, within RR42_RS18600 at 4028.186 kb on + strand, within RR42_RS18600 at 4028.187 kb on - strand, within RR42_RS18600 at 4028.203 kb on + strand, within RR42_RS18600 at 4028.204 kb on - strand, within RR42_RS18600 at 4028.244 kb on + strand, within RR42_RS18600 at 4028.329 kb on + strand, within RR42_RS18600 at 4028.330 kb on - strand, within RR42_RS18600 at 4028.464 kb on + strand, within RR42_RS18600 at 4028.464 kb on + strand, within RR42_RS18600 at 4028.465 kb on - strand, within RR42_RS18600 at 4028.465 kb on - strand, within RR42_RS18600 at 4028.465 kb on - strand, within RR42_RS18600 at 4028.465 kb on - strand, within RR42_RS18600 at 4028.465 kb on - strand, within RR42_RS18600 at 4028.709 kb on + strand at 4028.709 kb on + strand at 4028.710 kb on - strand at 4028.710 kb on - strand at 4028.745 kb on + strand at 4028.746 kb on - strand at 4028.746 kb on - strand at 4028.790 kb on + strand, within RR42_RS18605 at 4028.791 kb on - strand, within RR42_RS18605 at 4028.930 kb on + strand, within RR42_RS18605 at 4028.991 kb on + strand, within RR42_RS18605 at 4029.348 kb on + strand, within RR42_RS18605 at 4029.348 kb on + strand, within RR42_RS18605 at 4029.429 kb on + strand, within RR42_RS18605 at 4029.489 kb on + strand at 4029.490 kb on - strand at 4029.490 kb on - strand at 4029.490 kb on - strand at 4029.508 kb on - strand at 4029.510 kb on + strand at 4029.510 kb on + strand at 4029.510 kb on + strand at 4029.510 kb on + strand at 4029.511 kb on - strand at 4029.511 kb on - strand at 4029.511 kb on - strand at 4029.511 kb on - strand at 4029.587 kb on - strand at 4029.600 kb on - strand at 4029.736 kb on + strand, within RR42_RS18610 at 4029.737 kb on - strand, within RR42_RS18610 at 4029.777 kb on + strand, within RR42_RS18610 at 4029.778 kb on - strand, within RR42_RS18610 at 4030.091 kb on + strand, within RR42_RS18615 at 4030.355 kb on + strand, within RR42_RS18615 at 4030.356 kb on - strand, within RR42_RS18615 at 4030.370 kb on + strand, within RR42_RS18615 at 4030.371 kb on - strand, within RR42_RS18615 at 4030.371 kb on - strand, within RR42_RS18615 at 4030.371 kb on - strand, within RR42_RS18615 at 4030.385 kb on + strand, within RR42_RS18615 at 4030.510 kb on + strand at 4030.510 kb on + strand at 4030.510 kb on + strand at 4030.510 kb on + strand at 4030.511 kb on - strand at 4030.511 kb on - strand at 4030.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 4,027,661 + +0.1 4,027,662 - -0.6 4,027,662 - +0.1 4,027,662 - +0.4 4,027,662 - -2.2 4,027,662 - +1.2 4,027,662 - -1.4 4,027,850 - -0.6 4,027,930 + RR42_RS18600 0.19 -0.4 4,027,930 + RR42_RS18600 0.19 +1.1 4,027,937 - RR42_RS18600 0.19 -0.7 4,027,977 + RR42_RS18600 0.24 +1.2 4,027,987 + RR42_RS18600 0.25 -1.8 4,028,021 + RR42_RS18600 0.29 -0.9 4,028,143 + RR42_RS18600 0.42 +0.1 4,028,143 + RR42_RS18600 0.42 +0.2 4,028,144 - RR42_RS18600 0.42 +0.5 4,028,144 - RR42_RS18600 0.42 +3.6 4,028,186 + RR42_RS18600 0.47 +0.3 4,028,186 + RR42_RS18600 0.47 +0.6 4,028,187 - RR42_RS18600 0.47 -1.0 4,028,203 + RR42_RS18600 0.49 -0.6 4,028,204 - RR42_RS18600 0.49 +1.1 4,028,244 + RR42_RS18600 0.54 -1.1 4,028,329 + RR42_RS18600 0.63 -0.4 4,028,330 - RR42_RS18600 0.63 +0.3 4,028,464 + RR42_RS18600 0.78 +1.5 4,028,464 + RR42_RS18600 0.78 +0.5 4,028,465 - RR42_RS18600 0.78 +1.6 4,028,465 - RR42_RS18600 0.78 +1.9 4,028,465 - RR42_RS18600 0.78 -0.1 4,028,465 - RR42_RS18600 0.78 +0.6 4,028,465 - RR42_RS18600 0.78 +1.1 4,028,709 + +0.1 4,028,709 + +1.4 4,028,710 - +1.0 4,028,710 - -0.6 4,028,745 + +0.6 4,028,746 - -0.4 4,028,746 - -0.1 4,028,790 + RR42_RS18605 0.15 +0.6 4,028,791 - RR42_RS18605 0.15 -0.7 4,028,930 + RR42_RS18605 0.31 +0.3 4,028,991 + RR42_RS18605 0.38 +1.8 4,029,348 + RR42_RS18605 0.78 +2.5 4,029,348 + RR42_RS18605 0.78 -0.7 4,029,429 + RR42_RS18605 0.87 +0.2 4,029,489 + -0.9 4,029,490 - -0.3 4,029,490 - -0.8 4,029,490 - +0.2 4,029,508 - +1.1 4,029,510 + -2.3 4,029,510 + -1.7 4,029,510 + -0.5 4,029,510 + -0.5 4,029,511 - +4.2 4,029,511 - +1.4 4,029,511 - +0.4 4,029,511 - -0.9 4,029,587 - -0.4 4,029,600 - -1.7 4,029,736 + RR42_RS18610 0.27 +1.3 4,029,737 - RR42_RS18610 0.28 -1.5 4,029,777 + RR42_RS18610 0.45 -0.5 4,029,778 - RR42_RS18610 0.46 -0.4 4,030,091 + RR42_RS18615 0.27 -0.2 4,030,355 + RR42_RS18615 0.80 +0.2 4,030,356 - RR42_RS18615 0.80 -0.2 4,030,370 + RR42_RS18615 0.83 -0.7 4,030,371 - RR42_RS18615 0.83 +0.1 4,030,371 - RR42_RS18615 0.83 -0.5 4,030,371 - RR42_RS18615 0.83 +0.1 4,030,385 + RR42_RS18615 0.86 +0.2 4,030,510 + +0.1 4,030,510 + -0.1 4,030,510 + +0.8 4,030,510 + +0.2 4,030,511 - +0.4 4,030,511 - +0.2 4,030,511 - -0.6
Or see this region's nucleotide sequence