Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17160 and RR42_RS17165 are separated by 19 nucleotides RR42_RS17165 and RR42_RS17170 are separated by 117 nucleotides RR42_RS17170 and RR42_RS17175 are separated by 189 nucleotides
RR42_RS17160: RR42_RS17160 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 3,715,572 to 3,716,588
_RS17160
RR42_RS17165: RR42_RS17165 - hypothetical protein, at 3,716,608 to 3,716,919
_RS17165
RR42_RS17170: RR42_RS17170 - argininosuccinate synthase, at 3,717,037 to 3,718,266
_RS17170
RR42_RS17175: RR42_RS17175 - ornithine carbamoyltransferase, at 3,718,456 to 3,719,379
_RS17175
Position (kb)
3717
3718
3719 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3716.586 kb on + strand at 3716.587 kb on - strand at 3716.627 kb on + strand at 3716.699 kb on + strand, within RR42_RS17165 at 3716.699 kb on + strand, within RR42_RS17165 at 3716.699 kb on + strand, within RR42_RS17165 at 3716.700 kb on - strand, within RR42_RS17165 at 3716.899 kb on + strand at 3716.900 kb on - strand at 3716.900 kb on - strand at 3716.900 kb on - strand at 3716.922 kb on + strand at 3716.922 kb on + strand at 3716.922 kb on + strand at 3716.922 kb on + strand at 3718.288 kb on - strand at 3718.460 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 3,716,586 + -1.2 3,716,587 - -1.0 3,716,627 + -0.9 3,716,699 + RR42_RS17165 0.29 -0.1 3,716,699 + RR42_RS17165 0.29 +3.5 3,716,699 + RR42_RS17165 0.29 +2.5 3,716,700 - RR42_RS17165 0.29 -1.7 3,716,899 + +0.8 3,716,900 - +1.2 3,716,900 - +0.5 3,716,900 - -0.4 3,716,922 + -0.5 3,716,922 + -0.1 3,716,922 + +0.3 3,716,922 + -0.5 3,718,288 - +0.9 3,718,460 + +0.3
Or see this region's nucleotide sequence