Experiment: R2A with Paraquat dichloride 0.064 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt RR42_RS15445 and RR42_RS15450 are separated by 153 nucleotides RR42_RS15450 and RR42_RS15455 are separated by 18 nucleotides RR42_RS15455 and RR42_RS15460 are separated by 26 nucleotides 
        RR42_RS15445: RR42_RS15445 - 7-cyano-7-deazaguanine synthase, at 3,337,877 to 3,338,560 
        _RS15445 
         
        
        RR42_RS15450: RR42_RS15450 - Tol system periplasmic component YbgF, at 3,338,714 to 3,339,472 
        _RS15450 
         
        
        RR42_RS15455: RR42_RS15455 - membrane protein, at 3,339,491 to 3,339,997 
        _RS15455 
         
        
        RR42_RS15460: RR42_RS15460 - translocation protein TolB, at 3,340,024 to 3,341,352 
        _RS15460 
         Position (kb) 3339 
3340 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 at 3338.634 kb on + strand at 3338.635 kb on - strand at 3338.659 kb on - strand at 3338.659 kb on - strand at 3338.663 kb on + strand at 3338.664 kb on - strand at 3338.664 kb on - strand at 3338.673 kb on + strand at 3338.673 kb on + strand at 3338.674 kb on - strand at 3338.674 kb on - strand at 3338.715 kb on + strand at 3338.715 kb on + strand at 3338.716 kb on - strand at 3338.769 kb on + strand at 3338.769 kb on + strand at 3338.778 kb on - strand at 3338.778 kb on - strand at 3338.880 kb on + strand, within RR42_RS15450 at 3338.881 kb on - strand, within RR42_RS15450 at 3338.922 kb on + strand, within RR42_RS15450 at 3338.976 kb on + strand, within RR42_RS15450 at 3338.977 kb on - strand, within RR42_RS15450 at 3339.069 kb on + strand, within RR42_RS15450 at 3339.070 kb on - strand, within RR42_RS15450 at 3339.166 kb on - strand, within RR42_RS15450 at 3339.439 kb on + strand at 3339.439 kb on + strand at 3339.439 kb on + strand at 3339.492 kb on + strand at 3339.493 kb on - strand at 3339.493 kb on - strand at 3339.493 kb on - strand at 3339.495 kb on + strand at 3339.495 kb on + strand at 3339.495 kb on + strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.496 kb on - strand at 3339.663 kb on + strand, within RR42_RS15455 at 3339.762 kb on + strand, within RR42_RS15455 at 3339.780 kb on + strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.781 kb on - strand, within RR42_RS15455 at 3339.798 kb on + strand, within RR42_RS15455 at 3339.799 kb on - strand, within RR42_RS15455 at 3339.892 kb on + strand, within RR42_RS15455 at 3339.997 kb on + strand at 3340.011 kb on - strand at 3340.026 kb on - strand at 3340.026 kb on - strand at 3340.319 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.418 kb on + strand, within RR42_RS15460 at 3340.419 kb on + strand, within RR42_RS15460 at 3340.525 kb on - strand, within RR42_RS15460 at 3340.679 kb on + strand, within RR42_RS15460 at 3340.679 kb on + strand, within RR42_RS15460 at 3340.680 kb on - strand, within RR42_RS15460 at 3340.680 kb on - strand, within RR42_RS15460 at 3340.811 kb on + strand, within RR42_RS15460 at 3340.812 kb on - strand, within RR42_RS15460 at 3340.956 kb on - strand, within RR42_RS15460 at 3340.956 kb on - strand, within RR42_RS15460 at 3340.992 kb on + strand, within RR42_RS15460  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 3,338,634 +  -0.3 3,338,635 -  -1.3 3,338,659 -  -2.3 3,338,659 -  -1.4 3,338,663 +  -1.1 3,338,664 -  +0.7 3,338,664 -  +1.1 3,338,673 +  +0.8 3,338,673 +  -0.0 3,338,674 -  +2.4 3,338,674 -  +1.1 3,338,715 +  +1.0 3,338,715 +  -2.1 3,338,716 -  +0.1 3,338,769 +  -0.2 3,338,769 +  -0.8 3,338,778 -  +0.1 3,338,778 -  +1.0 3,338,880 +  RR42_RS15450 0.22  +0.1 3,338,881 -  RR42_RS15450 0.22  -0.7 3,338,922 +  RR42_RS15450 0.27  +0.4 3,338,976 +  RR42_RS15450 0.35  +0.2 3,338,977 -  RR42_RS15450 0.35  +0.1 3,339,069 +  RR42_RS15450 0.47  +0.4 3,339,070 -  RR42_RS15450 0.47  -0.8 3,339,166 -  RR42_RS15450 0.60  -0.5 3,339,439 +  -1.3 3,339,439 +  -0.5 3,339,439 +  -0.7 3,339,492 +  -2.0 3,339,493 -  -1.5 3,339,493 -  -1.7 3,339,493 -  +0.4 3,339,495 +  -1.5 3,339,495 +  -0.2 3,339,495 +  -1.6 3,339,496 -  +0.4 3,339,496 -  -0.1 3,339,496 -  -1.4 3,339,496 -  -0.1 3,339,663 +  RR42_RS15455 0.34  +1.1 3,339,762 +  RR42_RS15455 0.53  -0.8 3,339,780 +  RR42_RS15455 0.57  -1.8 3,339,781 -  RR42_RS15455 0.57  -2.9 3,339,781 -  RR42_RS15455 0.57  -2.2 3,339,781 -  RR42_RS15455 0.57  -1.1 3,339,781 -  RR42_RS15455 0.57  +0.1 3,339,781 -  RR42_RS15455 0.57  -0.9 3,339,798 +  RR42_RS15455 0.61  -2.1 3,339,799 -  RR42_RS15455 0.61  -1.9 3,339,892 +  RR42_RS15455 0.79  +0.8 3,339,997 +  -2.0 3,340,011 -  -0.4 3,340,026 -  -1.4 3,340,026 -  -1.4 3,340,319 +  RR42_RS15460 0.22  +0.4 3,340,418 +  RR42_RS15460 0.30  +0.4 3,340,418 +  RR42_RS15460 0.30  -1.1 3,340,418 +  RR42_RS15460 0.30  +2.4 3,340,419 +  RR42_RS15460 0.30  -0.4 3,340,525 -  RR42_RS15460 0.38  +0.5 3,340,679 +  RR42_RS15460 0.49  -0.1 3,340,679 +  RR42_RS15460 0.49  -2.3 3,340,680 -  RR42_RS15460 0.49  -0.4 3,340,680 -  RR42_RS15460 0.49  -0.3 3,340,811 +  RR42_RS15460 0.59  +0.7 3,340,812 -  RR42_RS15460 0.59  +0.5 3,340,956 -  RR42_RS15460 0.70  +0.8 3,340,956 -  RR42_RS15460 0.70  -1.3 3,340,992 +  RR42_RS15460 0.73  -1.3 
Or see this region's nucleotide sequence