Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14695 and RR42_RS14700 are separated by 8 nucleotides RR42_RS14700 and RR42_RS14705 are separated by 316 nucleotides RR42_RS14705 and RR42_RS14710 are separated by 77 nucleotides RR42_RS14710 and RR42_RS14715 are separated by 106 nucleotides
RR42_RS14695: RR42_RS14695 - membrane protein, at 3,167,457 to 3,168,650
_RS14695
RR42_RS14700: RR42_RS14700 - hypothetical protein, at 3,168,659 to 3,169,126
_RS14700
RR42_RS14705: RR42_RS14705 - multidrug transporter, at 3,169,443 to 3,169,775
_RS14705
RR42_RS14710: RR42_RS14710 - tRNA-Arg, at 3,169,853 to 3,169,927
_RS14710
RR42_RS14715: RR42_RS14715 - hypothetical protein, at 3,170,034 to 3,170,429
_RS14715
Position (kb)
3169
3170 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3168.660 kb on + strand at 3168.660 kb on + strand at 3168.661 kb on - strand at 3168.661 kb on - strand at 3168.661 kb on - strand at 3168.662 kb on + strand at 3168.663 kb on - strand at 3168.663 kb on - strand at 3168.663 kb on - strand at 3168.663 kb on - strand at 3168.663 kb on - strand at 3168.802 kb on - strand, within RR42_RS14700 at 3168.802 kb on - strand, within RR42_RS14700 at 3168.822 kb on + strand, within RR42_RS14700 at 3169.032 kb on - strand, within RR42_RS14700 at 3169.276 kb on + strand at 3169.276 kb on + strand at 3169.277 kb on - strand at 3169.277 kb on - strand at 3169.369 kb on + strand at 3169.369 kb on + strand at 3169.370 kb on - strand at 3169.370 kb on - strand at 3169.370 kb on - strand at 3169.452 kb on + strand at 3169.452 kb on + strand at 3169.452 kb on + strand at 3169.452 kb on + strand at 3169.453 kb on - strand at 3169.453 kb on - strand at 3169.453 kb on - strand at 3169.453 kb on - strand at 3169.456 kb on + strand at 3169.456 kb on + strand at 3169.456 kb on + strand at 3169.456 kb on + strand at 3169.457 kb on - strand at 3169.457 kb on - strand at 3169.457 kb on - strand at 3169.457 kb on - strand at 3169.457 kb on - strand at 3169.459 kb on + strand at 3169.560 kb on + strand, within RR42_RS14705 at 3169.560 kb on + strand, within RR42_RS14705 at 3169.609 kb on + strand, within RR42_RS14705 at 3169.609 kb on + strand, within RR42_RS14705 at 3169.610 kb on - strand, within RR42_RS14705 at 3169.725 kb on - strand, within RR42_RS14705 at 3169.725 kb on - strand, within RR42_RS14705 at 3169.933 kb on - strand at 3169.992 kb on - strand at 3169.994 kb on - strand at 3169.994 kb on - strand at 3170.176 kb on + strand, within RR42_RS14715 at 3170.176 kb on + strand, within RR42_RS14715 at 3170.182 kb on + strand, within RR42_RS14715 at 3170.182 kb on + strand, within RR42_RS14715 at 3170.183 kb on - strand, within RR42_RS14715 at 3170.183 kb on - strand, within RR42_RS14715 at 3170.272 kb on + strand, within RR42_RS14715 at 3170.272 kb on + strand, within RR42_RS14715 at 3170.302 kb on + strand, within RR42_RS14715 at 3170.303 kb on - strand, within RR42_RS14715 at 3170.397 kb on + strand at 3170.398 kb on - strand at 3170.429 kb on + strand at 3170.429 kb on + strand at 3170.430 kb on - strand at 3170.430 kb on - strand at 3170.469 kb on + strand at 3170.496 kb on - strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.519 kb on + strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.520 kb on - strand at 3170.655 kb on - strand at 3170.772 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 3,168,660 + +2.3 3,168,660 + +1.0 3,168,661 - +0.3 3,168,661 - +1.3 3,168,661 - +0.1 3,168,662 + +0.9 3,168,663 - -0.3 3,168,663 - -0.1 3,168,663 - +0.0 3,168,663 - -0.0 3,168,663 - -1.8 3,168,802 - RR42_RS14700 0.31 +0.9 3,168,802 - RR42_RS14700 0.31 -0.9 3,168,822 + RR42_RS14700 0.35 +0.8 3,169,032 - RR42_RS14700 0.80 -1.3 3,169,276 + +1.6 3,169,276 + -0.7 3,169,277 - -0.3 3,169,277 - -0.4 3,169,369 + +0.5 3,169,369 + +0.3 3,169,370 - +0.1 3,169,370 - +0.3 3,169,370 - -1.7 3,169,452 + -0.0 3,169,452 + -0.2 3,169,452 + +0.1 3,169,452 + +0.5 3,169,453 - +1.3 3,169,453 - -0.6 3,169,453 - +0.2 3,169,453 - +0.5 3,169,456 + +0.5 3,169,456 + -0.4 3,169,456 + +0.3 3,169,456 + -1.2 3,169,457 - -0.6 3,169,457 - -0.4 3,169,457 - +1.6 3,169,457 - +0.2 3,169,457 - +0.7 3,169,459 + +0.0 3,169,560 + RR42_RS14705 0.35 +0.3 3,169,560 + RR42_RS14705 0.35 +1.0 3,169,609 + RR42_RS14705 0.50 -0.4 3,169,609 + RR42_RS14705 0.50 -0.3 3,169,610 - RR42_RS14705 0.50 +0.1 3,169,725 - RR42_RS14705 0.85 +0.3 3,169,725 - RR42_RS14705 0.85 -1.5 3,169,933 - +1.9 3,169,992 - -0.4 3,169,994 - -0.3 3,169,994 - -0.1 3,170,176 + RR42_RS14715 0.36 -0.9 3,170,176 + RR42_RS14715 0.36 -0.7 3,170,182 + RR42_RS14715 0.37 +0.2 3,170,182 + RR42_RS14715 0.37 +0.3 3,170,183 - RR42_RS14715 0.38 -0.4 3,170,183 - RR42_RS14715 0.38 -0.1 3,170,272 + RR42_RS14715 0.60 -0.5 3,170,272 + RR42_RS14715 0.60 +0.5 3,170,302 + RR42_RS14715 0.68 -1.2 3,170,303 - RR42_RS14715 0.68 +0.3 3,170,397 + -2.6 3,170,398 - +2.6 3,170,429 + -2.0 3,170,429 + -0.2 3,170,430 - +1.3 3,170,430 - -0.0 3,170,469 + -0.2 3,170,496 - +1.6 3,170,519 + +0.2 3,170,519 + -0.2 3,170,519 + +0.3 3,170,519 + -0.1 3,170,520 - +0.5 3,170,520 - -0.3 3,170,520 - -0.0 3,170,520 - -0.5 3,170,520 - +0.7 3,170,655 - +1.1 3,170,772 - -0.2
Or see this region's nucleotide sequence