Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14385 and RR42_RS14390 are separated by 40 nucleotides RR42_RS14390 and RR42_RS14395 are separated by 10 nucleotides RR42_RS14395 and RR42_RS14400 are separated by 16 nucleotides
RR42_RS14385: RR42_RS14385 - 3-isopropylmalate dehydrogenase, at 3,094,616 to 3,095,683
_RS14385
RR42_RS14390: RR42_RS14390 - 3-isopropylmalate dehydratase, at 3,095,724 to 3,096,374
_RS14390
RR42_RS14395: RR42_RS14395 - entericidin, at 3,096,385 to 3,096,516
_RS14395
RR42_RS14400: RR42_RS14400 - isopropylmalate isomerase, at 3,096,533 to 3,097,942
_RS14400
Position (kb)
3096
3097 Strain fitness (log2 ratio)
-2
-1
0
1 at 3095.466 kb on + strand, within RR42_RS14385 at 3095.467 kb on - strand, within RR42_RS14385 at 3095.779 kb on + strand at 3095.780 kb on - strand at 3095.780 kb on - strand at 3095.780 kb on - strand at 3095.780 kb on - strand at 3095.780 kb on - strand at 3095.845 kb on + strand, within RR42_RS14390 at 3095.846 kb on - strand, within RR42_RS14390 at 3096.013 kb on + strand, within RR42_RS14390 at 3096.070 kb on + strand, within RR42_RS14390 at 3096.358 kb on - strand at 3096.358 kb on - strand at 3096.368 kb on + strand at 3096.369 kb on - strand at 3096.386 kb on + strand at 3096.386 kb on + strand at 3096.387 kb on - strand at 3096.453 kb on + strand, within RR42_RS14395 at 3096.454 kb on - strand, within RR42_RS14395 at 3096.969 kb on + strand, within RR42_RS14400 at 3096.970 kb on - strand, within RR42_RS14400 at 3096.970 kb on - strand, within RR42_RS14400 at 3097.218 kb on + strand, within RR42_RS14400 at 3097.219 kb on - strand, within RR42_RS14400 at 3097.347 kb on + strand, within RR42_RS14400 at 3097.348 kb on - strand, within RR42_RS14400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 3,095,466 + RR42_RS14385 0.80 +0.6 3,095,467 - RR42_RS14385 0.80 +0.9 3,095,779 + -0.7 3,095,780 - +0.3 3,095,780 - +0.2 3,095,780 - +0.3 3,095,780 - +0.1 3,095,780 - +1.1 3,095,845 + RR42_RS14390 0.19 -0.5 3,095,846 - RR42_RS14390 0.19 -1.3 3,096,013 + RR42_RS14390 0.44 -0.5 3,096,070 + RR42_RS14390 0.53 -0.5 3,096,358 - +1.0 3,096,358 - -1.3 3,096,368 + -1.4 3,096,369 - +0.7 3,096,386 + -1.4 3,096,386 + +0.8 3,096,387 - +0.1 3,096,453 + RR42_RS14395 0.52 +1.7 3,096,454 - RR42_RS14395 0.52 -2.6 3,096,969 + RR42_RS14400 0.31 -0.1 3,096,970 - RR42_RS14400 0.31 -0.9 3,096,970 - RR42_RS14400 0.31 -0.5 3,097,218 + RR42_RS14400 0.49 -0.3 3,097,219 - RR42_RS14400 0.49 +0.9 3,097,347 + RR42_RS14400 0.58 +0.8 3,097,348 - RR42_RS14400 0.58 -0.5
Or see this region's nucleotide sequence