Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13040 and RR42_RS13045 are separated by 99 nucleotides RR42_RS13045 and RR42_RS13050 are separated by 174 nucleotides RR42_RS13050 and RR42_RS13055 are separated by 58 nucleotides RR42_RS13055 and RR42_RS13060 are separated by 29 nucleotides
RR42_RS13040: RR42_RS13040 - iron transporter, at 2,810,165 to 2,810,440
_RS13040
RR42_RS13045: RR42_RS13045 - ribose-5-phosphate isomerase A, at 2,810,540 to 2,811,226
_RS13045
RR42_RS13050: RR42_RS13050 - transaldolase, at 2,811,401 to 2,812,339
_RS13050
RR42_RS13055: RR42_RS13055 - NAD-dependent protein deacylase, at 2,812,398 to 2,813,144
_RS13055
RR42_RS13060: RR42_RS13060 - 23S rRNA methyltransferase, at 2,813,174 to 2,813,917
_RS13060
Position (kb)
2811
2812
2813 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2810.417 kb on + strand at 2810.418 kb on - strand at 2810.418 kb on - strand at 2810.418 kb on - strand at 2811.261 kb on - strand at 2811.308 kb on + strand at 2811.309 kb on + strand at 2811.309 kb on + strand at 2811.310 kb on - strand at 2811.310 kb on - strand at 2811.382 kb on + strand at 2811.382 kb on + strand at 2811.383 kb on - strand at 2811.476 kb on + strand at 2811.477 kb on - strand at 2811.539 kb on + strand, within RR42_RS13050 at 2811.540 kb on - strand, within RR42_RS13050 at 2811.728 kb on + strand, within RR42_RS13050 at 2811.728 kb on + strand, within RR42_RS13050 at 2811.728 kb on + strand, within RR42_RS13050 at 2811.728 kb on + strand, within RR42_RS13050 at 2811.729 kb on - strand, within RR42_RS13050 at 2811.729 kb on - strand, within RR42_RS13050 at 2811.835 kb on + strand, within RR42_RS13050 at 2811.835 kb on + strand, within RR42_RS13050 at 2811.835 kb on + strand, within RR42_RS13050 at 2811.835 kb on + strand, within RR42_RS13050 at 2811.835 kb on + strand, within RR42_RS13050 at 2811.836 kb on - strand, within RR42_RS13050 at 2812.013 kb on + strand, within RR42_RS13050 at 2812.014 kb on - strand, within RR42_RS13050 at 2812.014 kb on - strand, within RR42_RS13050 at 2812.014 kb on - strand, within RR42_RS13050 at 2812.014 kb on - strand, within RR42_RS13050 at 2812.019 kb on + strand, within RR42_RS13050 at 2812.020 kb on - strand, within RR42_RS13050 at 2812.202 kb on - strand, within RR42_RS13050 at 2812.331 kb on - strand at 2812.889 kb on + strand, within RR42_RS13055 at 2812.940 kb on - strand, within RR42_RS13055 at 2812.940 kb on - strand, within RR42_RS13055 at 2812.940 kb on - strand, within RR42_RS13055 at 2812.973 kb on - strand, within RR42_RS13055 at 2813.090 kb on + strand at 2813.090 kb on + strand at 2813.090 kb on + strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.146 kb on + strand at 2813.147 kb on - strand at 2813.208 kb on + strand at 2813.209 kb on - strand at 2813.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,810,417 + +0.1 2,810,418 - -1.0 2,810,418 - -2.1 2,810,418 - -1.1 2,811,261 - +0.1 2,811,308 + +2.2 2,811,309 + -0.1 2,811,309 + +3.2 2,811,310 - -0.6 2,811,310 - +0.4 2,811,382 + +0.2 2,811,382 + -2.0 2,811,383 - +1.0 2,811,476 + +0.6 2,811,477 - +0.4 2,811,539 + RR42_RS13050 0.15 -0.3 2,811,540 - RR42_RS13050 0.15 -0.6 2,811,728 + RR42_RS13050 0.35 -0.5 2,811,728 + RR42_RS13050 0.35 +1.0 2,811,728 + RR42_RS13050 0.35 +0.7 2,811,728 + RR42_RS13050 0.35 -1.9 2,811,729 - RR42_RS13050 0.35 -0.2 2,811,729 - RR42_RS13050 0.35 +1.0 2,811,835 + RR42_RS13050 0.46 +0.2 2,811,835 + RR42_RS13050 0.46 -0.6 2,811,835 + RR42_RS13050 0.46 +2.2 2,811,835 + RR42_RS13050 0.46 +0.2 2,811,835 + RR42_RS13050 0.46 -0.4 2,811,836 - RR42_RS13050 0.46 -0.2 2,812,013 + RR42_RS13050 0.65 -2.1 2,812,014 - RR42_RS13050 0.65 +0.4 2,812,014 - RR42_RS13050 0.65 +1.4 2,812,014 - RR42_RS13050 0.65 -1.3 2,812,014 - RR42_RS13050 0.65 +1.4 2,812,019 + RR42_RS13050 0.66 -0.8 2,812,020 - RR42_RS13050 0.66 -1.0 2,812,202 - RR42_RS13050 0.85 +0.4 2,812,331 - +0.4 2,812,889 + RR42_RS13055 0.66 -0.2 2,812,940 - RR42_RS13055 0.73 +0.6 2,812,940 - RR42_RS13055 0.73 -0.1 2,812,940 - RR42_RS13055 0.73 -1.0 2,812,973 - RR42_RS13055 0.77 -2.9 2,813,090 + -0.1 2,813,090 + -1.7 2,813,090 + +1.2 2,813,091 - -0.4 2,813,091 - +1.2 2,813,091 - +0.0 2,813,091 - +1.2 2,813,091 - +0.2 2,813,091 - -0.1 2,813,146 + +0.3 2,813,147 - -1.9 2,813,208 + +2.2 2,813,209 - +0.7 2,813,209 - +0.4
Or see this region's nucleotide sequence