Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12500 and RR42_RS12505 overlap by 4 nucleotides RR42_RS12505 and RR42_RS12510 are separated by 232 nucleotides RR42_RS12510 and RR42_RS12515 are separated by 47 nucleotides RR42_RS12515 and RR42_RS12520 are separated by 56 nucleotides RR42_RS12520 and RR42_RS12525 are separated by 1 nucleotides
RR42_RS12500: RR42_RS12500 - enoyl-CoA hydratase, at 2,688,624 to 2,689,403
_RS12500
RR42_RS12505: RR42_RS12505 - TetR family transcriptional regulator, at 2,689,400 to 2,690,005
_RS12505
RR42_RS12510: RR42_RS12510 - hydrolase, at 2,690,238 to 2,691,077
_RS12510
RR42_RS12515: RR42_RS12515 - camphor resistance protein CrcB, at 2,691,125 to 2,691,505
_RS12515
RR42_RS12520: RR42_RS12520 - molybdenum cofactor biosynthesis protein MoaE, at 2,691,562 to 2,692,032
_RS12520
RR42_RS12525: RR42_RS12525 - molybdenum cofactor biosynthesis protein MoaD, at 2,692,034 to 2,692,291
_RS12525
Position (kb)
2690
2691
2692 Strain fitness (log2 ratio)
-2
-1
0
1 at 2689.441 kb on + strand at 2689.486 kb on - strand, within RR42_RS12505 at 2689.591 kb on + strand, within RR42_RS12505 at 2689.594 kb on - strand, within RR42_RS12505 at 2689.617 kb on + strand, within RR42_RS12505 at 2689.618 kb on - strand, within RR42_RS12505 at 2689.626 kb on + strand, within RR42_RS12505 at 2689.627 kb on - strand, within RR42_RS12505 at 2689.627 kb on - strand, within RR42_RS12505 at 2689.630 kb on + strand, within RR42_RS12505 at 2689.630 kb on + strand, within RR42_RS12505 at 2689.630 kb on + strand, within RR42_RS12505 at 2689.833 kb on + strand, within RR42_RS12505 at 2689.834 kb on - strand, within RR42_RS12505 at 2689.839 kb on + strand, within RR42_RS12505 at 2689.839 kb on + strand, within RR42_RS12505 at 2689.839 kb on + strand, within RR42_RS12505 at 2689.839 kb on + strand, within RR42_RS12505 at 2689.840 kb on - strand, within RR42_RS12505 at 2689.840 kb on - strand, within RR42_RS12505 at 2689.896 kb on + strand, within RR42_RS12505 at 2689.896 kb on + strand, within RR42_RS12505 at 2689.896 kb on + strand, within RR42_RS12505 at 2689.897 kb on - strand, within RR42_RS12505 at 2689.899 kb on + strand, within RR42_RS12505 at 2689.899 kb on + strand, within RR42_RS12505 at 2690.142 kb on - strand at 2690.149 kb on + strand at 2690.149 kb on + strand at 2690.149 kb on + strand at 2690.149 kb on + strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.536 kb on + strand, within RR42_RS12510 at 2690.536 kb on + strand, within RR42_RS12510 at 2690.536 kb on + strand, within RR42_RS12510 at 2690.536 kb on + strand, within RR42_RS12510 at 2690.537 kb on - strand, within RR42_RS12510 at 2690.679 kb on + strand, within RR42_RS12510 at 2690.680 kb on - strand, within RR42_RS12510 at 2690.944 kb on - strand, within RR42_RS12510 at 2691.051 kb on - strand at 2691.141 kb on + strand at 2691.142 kb on - strand at 2691.216 kb on + strand, within RR42_RS12515 at 2691.217 kb on - strand, within RR42_RS12515 at 2691.689 kb on + strand, within RR42_RS12520 at 2691.690 kb on - strand, within RR42_RS12520 at 2691.690 kb on - strand, within RR42_RS12520 at 2691.875 kb on + strand, within RR42_RS12520 at 2691.875 kb on + strand, within RR42_RS12520 at 2691.979 kb on + strand, within RR42_RS12520 at 2691.979 kb on + strand, within RR42_RS12520 at 2691.980 kb on - strand, within RR42_RS12520 at 2692.019 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,689,441 + +0.6 2,689,486 - RR42_RS12505 0.14 -0.6 2,689,591 + RR42_RS12505 0.32 -0.4 2,689,594 - RR42_RS12505 0.32 -0.2 2,689,617 + RR42_RS12505 0.36 -0.1 2,689,618 - RR42_RS12505 0.36 -2.3 2,689,626 + RR42_RS12505 0.37 +0.2 2,689,627 - RR42_RS12505 0.37 -0.9 2,689,627 - RR42_RS12505 0.37 +0.6 2,689,630 + RR42_RS12505 0.38 -0.4 2,689,630 + RR42_RS12505 0.38 +1.2 2,689,630 + RR42_RS12505 0.38 -0.4 2,689,833 + RR42_RS12505 0.71 -0.4 2,689,834 - RR42_RS12505 0.72 -1.6 2,689,839 + RR42_RS12505 0.72 -0.2 2,689,839 + RR42_RS12505 0.72 -0.5 2,689,839 + RR42_RS12505 0.72 -0.8 2,689,839 + RR42_RS12505 0.72 -0.5 2,689,840 - RR42_RS12505 0.73 -0.6 2,689,840 - RR42_RS12505 0.73 -0.3 2,689,896 + RR42_RS12505 0.82 -0.7 2,689,896 + RR42_RS12505 0.82 +0.7 2,689,896 + RR42_RS12505 0.82 +1.2 2,689,897 - RR42_RS12505 0.82 -0.4 2,689,899 + RR42_RS12505 0.82 -1.2 2,689,899 + RR42_RS12505 0.82 -0.3 2,690,142 - +0.8 2,690,149 + +0.6 2,690,149 + +1.2 2,690,149 + +0.3 2,690,149 + -1.6 2,690,150 - +0.0 2,690,150 - +0.8 2,690,150 - +0.8 2,690,150 - +0.4 2,690,150 - +0.3 2,690,150 - +0.8 2,690,150 - -2.0 2,690,536 + RR42_RS12510 0.35 +0.4 2,690,536 + RR42_RS12510 0.35 -2.7 2,690,536 + RR42_RS12510 0.35 +1.2 2,690,536 + RR42_RS12510 0.35 -1.0 2,690,537 - RR42_RS12510 0.36 -1.3 2,690,679 + RR42_RS12510 0.53 -0.2 2,690,680 - RR42_RS12510 0.53 +1.4 2,690,944 - RR42_RS12510 0.84 -0.8 2,691,051 - -0.8 2,691,141 + -0.3 2,691,142 - -1.2 2,691,216 + RR42_RS12515 0.24 -0.5 2,691,217 - RR42_RS12515 0.24 -1.2 2,691,689 + RR42_RS12520 0.27 +1.5 2,691,690 - RR42_RS12520 0.27 +0.2 2,691,690 - RR42_RS12520 0.27 +0.9 2,691,875 + RR42_RS12520 0.66 +1.7 2,691,875 + RR42_RS12520 0.66 +0.9 2,691,979 + RR42_RS12520 0.89 +0.4 2,691,979 + RR42_RS12520 0.89 -0.3 2,691,980 - RR42_RS12520 0.89 +0.5 2,692,019 - -0.0
Or see this region's nucleotide sequence