Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS12510

Experiment: R2A with Paraquat dichloride 0.064 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS12500 and RR42_RS12505 overlap by 4 nucleotidesRR42_RS12505 and RR42_RS12510 are separated by 232 nucleotidesRR42_RS12510 and RR42_RS12515 are separated by 47 nucleotidesRR42_RS12515 and RR42_RS12520 are separated by 56 nucleotidesRR42_RS12520 and RR42_RS12525 are separated by 1 nucleotides RR42_RS12500: RR42_RS12500 - enoyl-CoA hydratase, at 2,688,624 to 2,689,403 _RS12500 RR42_RS12505: RR42_RS12505 - TetR family transcriptional regulator, at 2,689,400 to 2,690,005 _RS12505 RR42_RS12510: RR42_RS12510 - hydrolase, at 2,690,238 to 2,691,077 _RS12510 RR42_RS12515: RR42_RS12515 - camphor resistance protein CrcB, at 2,691,125 to 2,691,505 _RS12515 RR42_RS12520: RR42_RS12520 - molybdenum cofactor biosynthesis protein MoaE, at 2,691,562 to 2,692,032 _RS12520 RR42_RS12525: RR42_RS12525 - molybdenum cofactor biosynthesis protein MoaD, at 2,692,034 to 2,692,291 _RS12525 Position (kb) 2690 2691 2692Strain fitness (log2 ratio) -2 -1 0 1at 2689.441 kb on + strandat 2689.486 kb on - strand, within RR42_RS12505at 2689.591 kb on + strand, within RR42_RS12505at 2689.594 kb on - strand, within RR42_RS12505at 2689.617 kb on + strand, within RR42_RS12505at 2689.618 kb on - strand, within RR42_RS12505at 2689.626 kb on + strand, within RR42_RS12505at 2689.627 kb on - strand, within RR42_RS12505at 2689.627 kb on - strand, within RR42_RS12505at 2689.630 kb on + strand, within RR42_RS12505at 2689.630 kb on + strand, within RR42_RS12505at 2689.630 kb on + strand, within RR42_RS12505at 2689.833 kb on + strand, within RR42_RS12505at 2689.834 kb on - strand, within RR42_RS12505at 2689.839 kb on + strand, within RR42_RS12505at 2689.839 kb on + strand, within RR42_RS12505at 2689.839 kb on + strand, within RR42_RS12505at 2689.839 kb on + strand, within RR42_RS12505at 2689.840 kb on - strand, within RR42_RS12505at 2689.840 kb on - strand, within RR42_RS12505at 2689.896 kb on + strand, within RR42_RS12505at 2689.896 kb on + strand, within RR42_RS12505at 2689.896 kb on + strand, within RR42_RS12505at 2689.897 kb on - strand, within RR42_RS12505at 2689.899 kb on + strand, within RR42_RS12505at 2689.899 kb on + strand, within RR42_RS12505at 2690.142 kb on - strandat 2690.149 kb on + strandat 2690.149 kb on + strandat 2690.149 kb on + strandat 2690.149 kb on + strandat 2690.150 kb on - strandat 2690.150 kb on - strandat 2690.150 kb on - strandat 2690.150 kb on - strandat 2690.150 kb on - strandat 2690.150 kb on - strandat 2690.150 kb on - strandat 2690.536 kb on + strand, within RR42_RS12510at 2690.536 kb on + strand, within RR42_RS12510at 2690.536 kb on + strand, within RR42_RS12510at 2690.536 kb on + strand, within RR42_RS12510at 2690.537 kb on - strand, within RR42_RS12510at 2690.679 kb on + strand, within RR42_RS12510at 2690.680 kb on - strand, within RR42_RS12510at 2690.944 kb on - strand, within RR42_RS12510at 2691.051 kb on - strandat 2691.141 kb on + strandat 2691.142 kb on - strandat 2691.216 kb on + strand, within RR42_RS12515at 2691.217 kb on - strand, within RR42_RS12515at 2691.689 kb on + strand, within RR42_RS12520at 2691.690 kb on - strand, within RR42_RS12520at 2691.690 kb on - strand, within RR42_RS12520at 2691.875 kb on + strand, within RR42_RS12520at 2691.875 kb on + strand, within RR42_RS12520at 2691.979 kb on + strand, within RR42_RS12520at 2691.979 kb on + strand, within RR42_RS12520at 2691.980 kb on - strand, within RR42_RS12520at 2692.019 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml
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2,689,441 + +0.6
2,689,486 - RR42_RS12505 0.14 -0.6
2,689,591 + RR42_RS12505 0.32 -0.4
2,689,594 - RR42_RS12505 0.32 -0.2
2,689,617 + RR42_RS12505 0.36 -0.1
2,689,618 - RR42_RS12505 0.36 -2.3
2,689,626 + RR42_RS12505 0.37 +0.2
2,689,627 - RR42_RS12505 0.37 -0.9
2,689,627 - RR42_RS12505 0.37 +0.6
2,689,630 + RR42_RS12505 0.38 -0.4
2,689,630 + RR42_RS12505 0.38 +1.2
2,689,630 + RR42_RS12505 0.38 -0.4
2,689,833 + RR42_RS12505 0.71 -0.4
2,689,834 - RR42_RS12505 0.72 -1.6
2,689,839 + RR42_RS12505 0.72 -0.2
2,689,839 + RR42_RS12505 0.72 -0.5
2,689,839 + RR42_RS12505 0.72 -0.8
2,689,839 + RR42_RS12505 0.72 -0.5
2,689,840 - RR42_RS12505 0.73 -0.6
2,689,840 - RR42_RS12505 0.73 -0.3
2,689,896 + RR42_RS12505 0.82 -0.7
2,689,896 + RR42_RS12505 0.82 +0.7
2,689,896 + RR42_RS12505 0.82 +1.2
2,689,897 - RR42_RS12505 0.82 -0.4
2,689,899 + RR42_RS12505 0.82 -1.2
2,689,899 + RR42_RS12505 0.82 -0.3
2,690,142 - +0.8
2,690,149 + +0.6
2,690,149 + +1.2
2,690,149 + +0.3
2,690,149 + -1.6
2,690,150 - +0.0
2,690,150 - +0.8
2,690,150 - +0.8
2,690,150 - +0.4
2,690,150 - +0.3
2,690,150 - +0.8
2,690,150 - -2.0
2,690,536 + RR42_RS12510 0.35 +0.4
2,690,536 + RR42_RS12510 0.35 -2.7
2,690,536 + RR42_RS12510 0.35 +1.2
2,690,536 + RR42_RS12510 0.35 -1.0
2,690,537 - RR42_RS12510 0.36 -1.3
2,690,679 + RR42_RS12510 0.53 -0.2
2,690,680 - RR42_RS12510 0.53 +1.4
2,690,944 - RR42_RS12510 0.84 -0.8
2,691,051 - -0.8
2,691,141 + -0.3
2,691,142 - -1.2
2,691,216 + RR42_RS12515 0.24 -0.5
2,691,217 - RR42_RS12515 0.24 -1.2
2,691,689 + RR42_RS12520 0.27 +1.5
2,691,690 - RR42_RS12520 0.27 +0.2
2,691,690 - RR42_RS12520 0.27 +0.9
2,691,875 + RR42_RS12520 0.66 +1.7
2,691,875 + RR42_RS12520 0.66 +0.9
2,691,979 + RR42_RS12520 0.89 +0.4
2,691,979 + RR42_RS12520 0.89 -0.3
2,691,980 - RR42_RS12520 0.89 +0.5
2,692,019 - -0.0

Or see this region's nucleotide sequence