Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12485 and RR42_RS12490 overlap by 8 nucleotides RR42_RS12490 and RR42_RS12495 are separated by 50 nucleotides RR42_RS12495 and RR42_RS12500 are separated by 112 nucleotides RR42_RS12500 and RR42_RS12505 overlap by 4 nucleotides
RR42_RS12485: RR42_RS12485 - ABC transporter ATP-binding protein, at 2,685,743 to 2,686,489
_RS12485
RR42_RS12490: RR42_RS12490 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,686,482 to 2,687,276
_RS12490
RR42_RS12495: RR42_RS12495 - ABC transporter permease, at 2,687,327 to 2,688,511
_RS12495
RR42_RS12500: RR42_RS12500 - enoyl-CoA hydratase, at 2,688,624 to 2,689,403
_RS12500
RR42_RS12505: RR42_RS12505 - TetR family transcriptional regulator, at 2,689,400 to 2,690,005
_RS12505
Position (kb)
2687
2688
2689 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2686.537 kb on + strand at 2686.537 kb on + strand at 2686.989 kb on + strand, within RR42_RS12490 at 2686.989 kb on + strand, within RR42_RS12490 at 2686.990 kb on - strand, within RR42_RS12490 at 2687.134 kb on + strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.301 kb on - strand at 2687.301 kb on - strand at 2687.301 kb on - strand at 2687.433 kb on + strand at 2687.433 kb on + strand at 2687.434 kb on - strand at 2687.634 kb on + strand, within RR42_RS12495 at 2687.634 kb on + strand, within RR42_RS12495 at 2687.685 kb on + strand, within RR42_RS12495 at 2687.686 kb on - strand, within RR42_RS12495 at 2687.691 kb on + strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.724 kb on + strand, within RR42_RS12495 at 2687.775 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.923 kb on - strand, within RR42_RS12495 at 2687.994 kb on + strand, within RR42_RS12495 at 2687.994 kb on + strand, within RR42_RS12495 at 2688.093 kb on + strand, within RR42_RS12495 at 2688.093 kb on + strand, within RR42_RS12495 at 2688.094 kb on - strand, within RR42_RS12495 at 2688.094 kb on - strand, within RR42_RS12495 at 2688.241 kb on - strand, within RR42_RS12495 at 2688.345 kb on + strand, within RR42_RS12495 at 2688.414 kb on + strand at 2688.415 kb on - strand at 2688.415 kb on - strand at 2688.626 kb on - strand at 2688.626 kb on - strand at 2688.626 kb on - strand at 2688.991 kb on + strand, within RR42_RS12500 at 2688.991 kb on + strand, within RR42_RS12500 at 2688.991 kb on + strand, within RR42_RS12500 at 2688.992 kb on - strand, within RR42_RS12500 at 2688.992 kb on - strand, within RR42_RS12500 at 2688.994 kb on + strand, within RR42_RS12500 at 2688.994 kb on + strand, within RR42_RS12500 at 2688.995 kb on - strand, within RR42_RS12500 at 2689.441 kb on + strand at 2689.486 kb on - strand, within RR42_RS12505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,686,537 + -1.3 2,686,537 + -0.4 2,686,989 + RR42_RS12490 0.64 +1.0 2,686,989 + RR42_RS12490 0.64 +0.1 2,686,990 - RR42_RS12490 0.64 -0.5 2,687,134 + RR42_RS12490 0.82 +0.4 2,687,135 - RR42_RS12490 0.82 -0.5 2,687,135 - RR42_RS12490 0.82 +1.0 2,687,135 - RR42_RS12490 0.82 -1.1 2,687,301 - +0.6 2,687,301 - +0.3 2,687,301 - +0.9 2,687,433 + +1.6 2,687,433 + +0.4 2,687,434 - +3.7 2,687,634 + RR42_RS12495 0.26 +0.6 2,687,634 + RR42_RS12495 0.26 +1.1 2,687,685 + RR42_RS12495 0.30 +0.1 2,687,686 - RR42_RS12495 0.30 +0.2 2,687,691 + RR42_RS12495 0.31 -1.3 2,687,692 - RR42_RS12495 0.31 -0.5 2,687,692 - RR42_RS12495 0.31 -0.8 2,687,692 - RR42_RS12495 0.31 +0.6 2,687,692 - RR42_RS12495 0.31 +0.7 2,687,724 + RR42_RS12495 0.34 -2.3 2,687,775 + RR42_RS12495 0.38 -0.0 2,687,922 + RR42_RS12495 0.50 -1.7 2,687,922 + RR42_RS12495 0.50 -0.8 2,687,922 + RR42_RS12495 0.50 -0.1 2,687,923 - RR42_RS12495 0.50 +1.2 2,687,994 + RR42_RS12495 0.56 -1.3 2,687,994 + RR42_RS12495 0.56 +0.3 2,688,093 + RR42_RS12495 0.65 +1.7 2,688,093 + RR42_RS12495 0.65 +1.2 2,688,094 - RR42_RS12495 0.65 +0.5 2,688,094 - RR42_RS12495 0.65 +2.7 2,688,241 - RR42_RS12495 0.77 +0.6 2,688,345 + RR42_RS12495 0.86 -0.5 2,688,414 + +0.1 2,688,415 - -0.4 2,688,415 - -0.6 2,688,626 - -2.1 2,688,626 - -1.7 2,688,626 - +1.4 2,688,991 + RR42_RS12500 0.47 -0.3 2,688,991 + RR42_RS12500 0.47 +0.8 2,688,991 + RR42_RS12500 0.47 -0.1 2,688,992 - RR42_RS12500 0.47 -0.0 2,688,992 - RR42_RS12500 0.47 -0.2 2,688,994 + RR42_RS12500 0.47 -0.5 2,688,994 + RR42_RS12500 0.47 -0.8 2,688,995 - RR42_RS12500 0.48 -0.5 2,689,441 + +0.6 2,689,486 - RR42_RS12505 0.14 -0.6
Or see this region's nucleotide sequence