Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12370 and RR42_RS12375 are separated by 274 nucleotides RR42_RS12375 and RR42_RS12380 are separated by 36 nucleotides RR42_RS12380 and RR42_RS12385 are separated by 118 nucleotides
RR42_RS12370: RR42_RS12370 - trans-aconitate methyltransferase, at 2,654,059 to 2,654,829
_RS12370
RR42_RS12375: RR42_RS12375 - LysR family transcriptional regulator, at 2,655,104 to 2,656,036
_RS12375
RR42_RS12380: RR42_RS12380 - hypothetical protein, at 2,656,073 to 2,656,396
_RS12380
RR42_RS12385: RR42_RS12385 - formamidase, at 2,656,515 to 2,657,747
_RS12385
Position (kb)
2655
2656
2657 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2654.150 kb on + strand, within RR42_RS12370 at 2654.150 kb on + strand, within RR42_RS12370 at 2654.163 kb on - strand, within RR42_RS12370 at 2654.163 kb on - strand, within RR42_RS12370 at 2654.319 kb on - strand, within RR42_RS12370 at 2654.322 kb on - strand, within RR42_RS12370 at 2654.726 kb on - strand, within RR42_RS12370 at 2654.784 kb on + strand at 2654.807 kb on + strand at 2654.807 kb on + strand at 2654.807 kb on + strand at 2654.807 kb on + strand at 2654.807 kb on + strand at 2654.807 kb on + strand at 2654.808 kb on - strand at 2654.808 kb on - strand at 2655.038 kb on + strand at 2655.039 kb on - strand at 2655.068 kb on + strand at 2655.722 kb on + strand, within RR42_RS12375 at 2655.722 kb on + strand, within RR42_RS12375 at 2655.723 kb on - strand, within RR42_RS12375 at 2655.723 kb on - strand, within RR42_RS12375 at 2655.881 kb on + strand, within RR42_RS12375 at 2656.074 kb on + strand at 2656.074 kb on + strand at 2656.075 kb on - strand at 2656.075 kb on - strand at 2656.380 kb on + strand at 2656.381 kb on - strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.387 kb on - strand at 2656.664 kb on + strand, within RR42_RS12385 at 2656.665 kb on - strand, within RR42_RS12385 at 2656.665 kb on - strand, within RR42_RS12385 at 2656.665 kb on - strand, within RR42_RS12385 at 2656.699 kb on + strand, within RR42_RS12385 at 2656.736 kb on - strand, within RR42_RS12385 at 2656.786 kb on + strand, within RR42_RS12385 at 2656.786 kb on + strand, within RR42_RS12385 at 2656.786 kb on + strand, within RR42_RS12385 at 2656.786 kb on + strand, within RR42_RS12385 at 2656.787 kb on - strand, within RR42_RS12385 at 2656.787 kb on - strand, within RR42_RS12385 at 2656.835 kb on - strand, within RR42_RS12385 at 2656.835 kb on - strand, within RR42_RS12385 at 2656.843 kb on + strand, within RR42_RS12385 at 2656.967 kb on + strand, within RR42_RS12385 at 2656.968 kb on - strand, within RR42_RS12385 at 2656.968 kb on - strand, within RR42_RS12385 at 2657.029 kb on + strand, within RR42_RS12385 at 2657.029 kb on + strand, within RR42_RS12385 at 2657.029 kb on + strand, within RR42_RS12385 at 2657.029 kb on + strand, within RR42_RS12385 at 2657.030 kb on - strand, within RR42_RS12385 at 2657.030 kb on - strand, within RR42_RS12385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,654,150 + RR42_RS12370 0.12 -0.9 2,654,150 + RR42_RS12370 0.12 +0.1 2,654,163 - RR42_RS12370 0.13 +0.8 2,654,163 - RR42_RS12370 0.13 +0.3 2,654,319 - RR42_RS12370 0.34 -0.5 2,654,322 - RR42_RS12370 0.34 -1.8 2,654,726 - RR42_RS12370 0.87 -0.6 2,654,784 + -0.7 2,654,807 + -0.2 2,654,807 + +1.1 2,654,807 + +0.6 2,654,807 + +0.7 2,654,807 + -0.7 2,654,807 + -0.2 2,654,808 - +0.6 2,654,808 - -1.2 2,655,038 + -0.2 2,655,039 - +0.2 2,655,068 + +1.6 2,655,722 + RR42_RS12375 0.66 +0.6 2,655,722 + RR42_RS12375 0.66 -0.7 2,655,723 - RR42_RS12375 0.66 -0.1 2,655,723 - RR42_RS12375 0.66 +1.3 2,655,881 + RR42_RS12375 0.83 +0.8 2,656,074 + +0.0 2,656,074 + -1.9 2,656,075 - -0.1 2,656,075 - -0.1 2,656,380 + -1.9 2,656,381 - -1.0 2,656,386 + -1.9 2,656,386 + +0.8 2,656,386 + -0.2 2,656,386 + -0.3 2,656,387 - -0.5 2,656,664 + RR42_RS12385 0.12 +1.6 2,656,665 - RR42_RS12385 0.12 +0.1 2,656,665 - RR42_RS12385 0.12 -0.7 2,656,665 - RR42_RS12385 0.12 -3.0 2,656,699 + RR42_RS12385 0.15 -0.1 2,656,736 - RR42_RS12385 0.18 +0.5 2,656,786 + RR42_RS12385 0.22 +0.2 2,656,786 + RR42_RS12385 0.22 +0.2 2,656,786 + RR42_RS12385 0.22 -1.4 2,656,786 + RR42_RS12385 0.22 +1.2 2,656,787 - RR42_RS12385 0.22 +0.3 2,656,787 - RR42_RS12385 0.22 +1.1 2,656,835 - RR42_RS12385 0.26 +0.9 2,656,835 - RR42_RS12385 0.26 -0.6 2,656,843 + RR42_RS12385 0.27 -0.0 2,656,967 + RR42_RS12385 0.37 -0.3 2,656,968 - RR42_RS12385 0.37 -0.8 2,656,968 - RR42_RS12385 0.37 -1.1 2,657,029 + RR42_RS12385 0.42 -0.4 2,657,029 + RR42_RS12385 0.42 -0.5 2,657,029 + RR42_RS12385 0.42 +0.1 2,657,029 + RR42_RS12385 0.42 +0.7 2,657,030 - RR42_RS12385 0.42 +0.5 2,657,030 - RR42_RS12385 0.42 -1.8
Or see this region's nucleotide sequence