Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12305 and RR42_RS12310 overlap by 67 nucleotides RR42_RS12310 and RR42_RS12315 are separated by 162 nucleotides RR42_RS12315 and RR42_RS12320 are separated by 16 nucleotides RR42_RS12320 and RR42_RS12325 are separated by 252 nucleotides
RR42_RS12305: RR42_RS12305 - hypothetical protein, at 2,643,042 to 2,643,341
_RS12305
RR42_RS12310: RR42_RS12310 - alkyl hydroperoxide reductase, at 2,643,275 to 2,643,946
_RS12310
RR42_RS12315: RR42_RS12315 - transcriptional regulator, at 2,644,109 to 2,645,002
_RS12315
RR42_RS12320: RR42_RS12320 - hypothetical protein, at 2,645,019 to 2,645,252
_RS12320
RR42_RS12325: RR42_RS12325 - glutathione S-transferase, at 2,645,505 to 2,646,140
_RS12325
Position (kb)
2644
2645
2646 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2643.339 kb on + strand at 2643.361 kb on - strand, within RR42_RS12310 at 2643.390 kb on + strand, within RR42_RS12310 at 2643.477 kb on + strand, within RR42_RS12310 at 2643.478 kb on - strand, within RR42_RS12310 at 2643.523 kb on + strand, within RR42_RS12310 at 2643.670 kb on + strand, within RR42_RS12310 at 2643.670 kb on + strand, within RR42_RS12310 at 2643.700 kb on - strand, within RR42_RS12310 at 2643.731 kb on + strand, within RR42_RS12310 at 2643.953 kb on + strand at 2643.971 kb on + strand at 2643.972 kb on - strand at 2643.972 kb on - strand at 2643.972 kb on - strand at 2644.052 kb on + strand at 2644.426 kb on + strand, within RR42_RS12315 at 2644.427 kb on - strand, within RR42_RS12315 at 2645.241 kb on - strand at 2645.332 kb on - strand at 2645.478 kb on + strand at 2645.478 kb on + strand at 2645.479 kb on - strand at 2645.479 kb on - strand at 2645.490 kb on - strand at 2645.572 kb on + strand, within RR42_RS12325 at 2645.680 kb on + strand, within RR42_RS12325 at 2645.680 kb on + strand, within RR42_RS12325 at 2645.681 kb on - strand, within RR42_RS12325 at 2645.681 kb on - strand, within RR42_RS12325 at 2645.740 kb on + strand, within RR42_RS12325 at 2645.756 kb on - strand, within RR42_RS12325 at 2645.908 kb on + strand, within RR42_RS12325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,643,339 + +0.6 2,643,361 - RR42_RS12310 0.13 -0.2 2,643,390 + RR42_RS12310 0.17 -0.5 2,643,477 + RR42_RS12310 0.30 +2.1 2,643,478 - RR42_RS12310 0.30 -0.2 2,643,523 + RR42_RS12310 0.37 -0.2 2,643,670 + RR42_RS12310 0.59 +0.7 2,643,670 + RR42_RS12310 0.59 -0.1 2,643,700 - RR42_RS12310 0.63 -1.8 2,643,731 + RR42_RS12310 0.68 +1.5 2,643,953 + +0.2 2,643,971 + -0.3 2,643,972 - -0.2 2,643,972 - -0.6 2,643,972 - +0.5 2,644,052 + +0.5 2,644,426 + RR42_RS12315 0.35 -0.7 2,644,427 - RR42_RS12315 0.36 -2.1 2,645,241 - -0.3 2,645,332 - -0.2 2,645,478 + -1.6 2,645,478 + +0.5 2,645,479 - +0.3 2,645,479 - -0.8 2,645,490 - +1.5 2,645,572 + RR42_RS12325 0.11 +1.1 2,645,680 + RR42_RS12325 0.28 -0.0 2,645,680 + RR42_RS12325 0.28 -0.1 2,645,681 - RR42_RS12325 0.28 -0.3 2,645,681 - RR42_RS12325 0.28 -1.6 2,645,740 + RR42_RS12325 0.37 -0.1 2,645,756 - RR42_RS12325 0.39 -0.2 2,645,908 + RR42_RS12325 0.63 +0.1
Or see this region's nucleotide sequence