Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12050 and RR42_RS12055 overlap by 4 nucleotides RR42_RS12055 and RR42_RS12060 are separated by 92 nucleotides RR42_RS12060 and RR42_RS12065 are separated by 195 nucleotides RR42_RS12065 and RR42_RS12070 are separated by 47 nucleotides
RR42_RS12050: RR42_RS12050 - DNA polymerase, at 2,583,750 to 2,584,703
_RS12050
RR42_RS12055: RR42_RS12055 - acyltransferase, at 2,584,700 to 2,585,194
_RS12055
RR42_RS12060: RR42_RS12060 - O-sialoglycoprotein endopeptidase, at 2,585,287 to 2,586,054
_RS12060
RR42_RS12065: RR42_RS12065 - acyl-CoA-binding protein, at 2,586,250 to 2,586,519
_RS12065
RR42_RS12070: RR42_RS12070 - MFS transporter, at 2,586,567 to 2,588,036
_RS12070
Position (kb)
2585
2586
2587 Strain fitness (log2 ratio)
-2
-1
0
1 at 2584.344 kb on - strand, within RR42_RS12050 at 2584.777 kb on - strand, within RR42_RS12055 at 2584.777 kb on - strand, within RR42_RS12055 at 2584.779 kb on + strand, within RR42_RS12055 at 2584.779 kb on + strand, within RR42_RS12055 at 2584.780 kb on - strand, within RR42_RS12055 at 2584.822 kb on - strand, within RR42_RS12055 at 2584.960 kb on + strand, within RR42_RS12055 at 2585.101 kb on - strand, within RR42_RS12055 at 2586.176 kb on + strand at 2586.176 kb on + strand at 2586.177 kb on - strand at 2586.177 kb on - strand at 2586.340 kb on + strand, within RR42_RS12065 at 2586.415 kb on + strand, within RR42_RS12065 at 2586.415 kb on + strand, within RR42_RS12065 at 2586.475 kb on + strand, within RR42_RS12065 at 2586.476 kb on - strand, within RR42_RS12065 at 2586.817 kb on + strand, within RR42_RS12070 at 2586.910 kb on + strand, within RR42_RS12070
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,584,344 - RR42_RS12050 0.62 +1.1 2,584,777 - RR42_RS12055 0.16 +0.1 2,584,777 - RR42_RS12055 0.16 -0.2 2,584,779 + RR42_RS12055 0.16 -0.2 2,584,779 + RR42_RS12055 0.16 -0.8 2,584,780 - RR42_RS12055 0.16 -0.1 2,584,822 - RR42_RS12055 0.25 -1.3 2,584,960 + RR42_RS12055 0.53 -1.2 2,585,101 - RR42_RS12055 0.81 +1.1 2,586,176 + -0.6 2,586,176 + +0.3 2,586,177 - -2.3 2,586,177 - +0.1 2,586,340 + RR42_RS12065 0.33 -0.5 2,586,415 + RR42_RS12065 0.61 -0.2 2,586,415 + RR42_RS12065 0.61 +0.2 2,586,475 + RR42_RS12065 0.83 -1.7 2,586,476 - RR42_RS12065 0.84 -1.3 2,586,817 + RR42_RS12070 0.17 +0.7 2,586,910 + RR42_RS12070 0.23 +0.6
Or see this region's nucleotide sequence