Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12045 and RR42_RS12050 overlap by 26 nucleotides RR42_RS12050 and RR42_RS12055 overlap by 4 nucleotides RR42_RS12055 and RR42_RS12060 are separated by 92 nucleotides
RR42_RS12045: RR42_RS12045 - hypothetical protein, at 2,582,609 to 2,583,775
_RS12045
RR42_RS12050: RR42_RS12050 - DNA polymerase, at 2,583,750 to 2,584,703
_RS12050
RR42_RS12055: RR42_RS12055 - acyltransferase, at 2,584,700 to 2,585,194
_RS12055
RR42_RS12060: RR42_RS12060 - O-sialoglycoprotein endopeptidase, at 2,585,287 to 2,586,054
_RS12060
Position (kb)
2584
2585
2586 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2583.777 kb on + strand at 2583.777 kb on + strand at 2583.778 kb on - strand at 2583.778 kb on - strand at 2583.778 kb on - strand at 2583.778 kb on - strand at 2583.853 kb on + strand, within RR42_RS12050 at 2583.853 kb on + strand, within RR42_RS12050 at 2583.853 kb on + strand, within RR42_RS12050 at 2583.880 kb on + strand, within RR42_RS12050 at 2583.880 kb on + strand, within RR42_RS12050 at 2583.880 kb on + strand, within RR42_RS12050 at 2583.881 kb on - strand, within RR42_RS12050 at 2583.884 kb on + strand, within RR42_RS12050 at 2583.885 kb on - strand, within RR42_RS12050 at 2584.004 kb on - strand, within RR42_RS12050 at 2584.121 kb on + strand, within RR42_RS12050 at 2584.121 kb on + strand, within RR42_RS12050 at 2584.121 kb on + strand, within RR42_RS12050 at 2584.121 kb on + strand, within RR42_RS12050 at 2584.122 kb on - strand, within RR42_RS12050 at 2584.122 kb on - strand, within RR42_RS12050 at 2584.344 kb on - strand, within RR42_RS12050 at 2584.777 kb on - strand, within RR42_RS12055 at 2584.777 kb on - strand, within RR42_RS12055 at 2584.779 kb on + strand, within RR42_RS12055 at 2584.779 kb on + strand, within RR42_RS12055 at 2584.780 kb on - strand, within RR42_RS12055 at 2584.822 kb on - strand, within RR42_RS12055 at 2584.960 kb on + strand, within RR42_RS12055 at 2585.101 kb on - strand, within RR42_RS12055 at 2586.176 kb on + strand at 2586.176 kb on + strand at 2586.177 kb on - strand at 2586.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,583,777 + +0.7 2,583,777 + +0.9 2,583,778 - -2.4 2,583,778 - +1.2 2,583,778 - -0.1 2,583,778 - -0.1 2,583,853 + RR42_RS12050 0.11 +0.3 2,583,853 + RR42_RS12050 0.11 -0.0 2,583,853 + RR42_RS12050 0.11 -0.9 2,583,880 + RR42_RS12050 0.14 -0.4 2,583,880 + RR42_RS12050 0.14 -0.5 2,583,880 + RR42_RS12050 0.14 +0.0 2,583,881 - RR42_RS12050 0.14 +0.2 2,583,884 + RR42_RS12050 0.14 -0.2 2,583,885 - RR42_RS12050 0.14 -0.7 2,584,004 - RR42_RS12050 0.27 +3.7 2,584,121 + RR42_RS12050 0.39 -0.2 2,584,121 + RR42_RS12050 0.39 -0.4 2,584,121 + RR42_RS12050 0.39 +1.9 2,584,121 + RR42_RS12050 0.39 +0.3 2,584,122 - RR42_RS12050 0.39 -0.6 2,584,122 - RR42_RS12050 0.39 -1.2 2,584,344 - RR42_RS12050 0.62 +1.1 2,584,777 - RR42_RS12055 0.16 +0.1 2,584,777 - RR42_RS12055 0.16 -0.2 2,584,779 + RR42_RS12055 0.16 -0.2 2,584,779 + RR42_RS12055 0.16 -0.8 2,584,780 - RR42_RS12055 0.16 -0.1 2,584,822 - RR42_RS12055 0.25 -1.3 2,584,960 + RR42_RS12055 0.53 -1.2 2,585,101 - RR42_RS12055 0.81 +1.1 2,586,176 + -0.6 2,586,176 + +0.3 2,586,177 - -2.3 2,586,177 - +0.1
Or see this region's nucleotide sequence