Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10975 and RR42_RS10980 are separated by 41 nucleotides RR42_RS10980 and RR42_RS10985 are separated by 49 nucleotides
RR42_RS10975: RR42_RS10975 - MFS transporter, at 2,344,119 to 2,345,450
_RS10975
RR42_RS10980: RR42_RS10980 - hydroxypyruvate isomerase, at 2,345,492 to 2,346,274
_RS10980
RR42_RS10985: RR42_RS10985 - NAD-dependent epimerase, at 2,346,324 to 2,347,313
_RS10985
Position (kb)
2345
2346
2347 Strain fitness (log2 ratio)
-2
-1
0
1 at 2344.545 kb on + strand, within RR42_RS10975 at 2344.554 kb on + strand, within RR42_RS10975 at 2344.671 kb on + strand, within RR42_RS10975 at 2344.821 kb on - strand, within RR42_RS10975 at 2344.917 kb on + strand, within RR42_RS10975 at 2344.918 kb on - strand, within RR42_RS10975 at 2344.918 kb on - strand, within RR42_RS10975 at 2344.942 kb on - strand, within RR42_RS10975 at 2345.162 kb on + strand, within RR42_RS10975 at 2345.162 kb on + strand, within RR42_RS10975 at 2345.162 kb on + strand, within RR42_RS10975 at 2345.162 kb on + strand, within RR42_RS10975 at 2345.325 kb on + strand at 2345.325 kb on + strand at 2345.325 kb on + strand at 2345.325 kb on + strand at 2345.326 kb on - strand at 2345.326 kb on - strand at 2345.326 kb on - strand at 2345.326 kb on - strand at 2345.326 kb on - strand at 2345.337 kb on + strand at 2345.338 kb on - strand at 2345.376 kb on + strand at 2345.376 kb on + strand at 2345.376 kb on + strand at 2345.377 kb on - strand at 2345.377 kb on - strand at 2345.377 kb on - strand at 2345.377 kb on - strand at 2345.377 kb on - strand at 2345.377 kb on - strand at 2345.377 kb on - strand at 2345.377 kb on - strand at 2345.525 kb on + strand at 2345.526 kb on - strand at 2345.526 kb on - strand at 2345.526 kb on - strand at 2345.591 kb on + strand, within RR42_RS10980 at 2345.768 kb on + strand, within RR42_RS10980 at 2345.855 kb on + strand, within RR42_RS10980 at 2345.948 kb on + strand, within RR42_RS10980 at 2346.029 kb on + strand, within RR42_RS10980 at 2346.152 kb on + strand, within RR42_RS10980 at 2346.159 kb on - strand, within RR42_RS10980 at 2346.261 kb on - strand at 2346.382 kb on + strand at 2346.382 kb on + strand at 2346.382 kb on + strand at 2346.382 kb on + strand at 2346.383 kb on - strand at 2346.383 kb on - strand at 2346.385 kb on + strand at 2346.386 kb on - strand at 2346.386 kb on - strand at 2346.720 kb on + strand, within RR42_RS10985 at 2346.721 kb on - strand, within RR42_RS10985 at 2346.822 kb on + strand, within RR42_RS10985 at 2347.187 kb on + strand, within RR42_RS10985 at 2347.188 kb on - strand, within RR42_RS10985
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,344,545 + RR42_RS10975 0.32 +0.6 2,344,554 + RR42_RS10975 0.33 -0.3 2,344,671 + RR42_RS10975 0.41 +0.5 2,344,821 - RR42_RS10975 0.53 -0.4 2,344,917 + RR42_RS10975 0.60 -1.7 2,344,918 - RR42_RS10975 0.60 -0.0 2,344,918 - RR42_RS10975 0.60 -1.5 2,344,942 - RR42_RS10975 0.62 -0.1 2,345,162 + RR42_RS10975 0.78 +0.2 2,345,162 + RR42_RS10975 0.78 -0.1 2,345,162 + RR42_RS10975 0.78 -0.3 2,345,162 + RR42_RS10975 0.78 +1.5 2,345,325 + +0.4 2,345,325 + +0.3 2,345,325 + -0.6 2,345,325 + -0.2 2,345,326 - -0.3 2,345,326 - -0.2 2,345,326 - +1.2 2,345,326 - -0.8 2,345,326 - -2.3 2,345,337 + -0.3 2,345,338 - -0.6 2,345,376 + +0.6 2,345,376 + -0.8 2,345,376 + +0.5 2,345,377 - -0.8 2,345,377 - -1.3 2,345,377 - +0.6 2,345,377 - +1.3 2,345,377 - +0.3 2,345,377 - +0.4 2,345,377 - -0.8 2,345,377 - -0.3 2,345,525 + -0.2 2,345,526 - +0.5 2,345,526 - +1.6 2,345,526 - -0.4 2,345,591 + RR42_RS10980 0.13 -0.8 2,345,768 + RR42_RS10980 0.35 +0.9 2,345,855 + RR42_RS10980 0.46 -1.1 2,345,948 + RR42_RS10980 0.58 +1.2 2,346,029 + RR42_RS10980 0.69 +1.2 2,346,152 + RR42_RS10980 0.84 +0.4 2,346,159 - RR42_RS10980 0.85 +1.7 2,346,261 - -1.6 2,346,382 + -1.3 2,346,382 + -0.3 2,346,382 + -1.4 2,346,382 + +0.7 2,346,383 - -1.8 2,346,383 - -0.3 2,346,385 + +0.4 2,346,386 - +1.0 2,346,386 - +1.0 2,346,720 + RR42_RS10985 0.40 +0.2 2,346,721 - RR42_RS10985 0.40 -0.1 2,346,822 + RR42_RS10985 0.50 -0.0 2,347,187 + RR42_RS10985 0.87 -0.6 2,347,188 - RR42_RS10985 0.87 +0.4
Or see this region's nucleotide sequence