Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10685 and RR42_RS10690 are separated by 652 nucleotides RR42_RS10690 and RR42_RS10695 are separated by 62 nucleotides RR42_RS10695 and RR42_RS10700 are separated by 56 nucleotides
RR42_RS10685: RR42_RS10685 - 3-oxoacyl-ACP reductase, at 2,281,159 to 2,281,626
_RS10685
RR42_RS10690: RR42_RS10690 - 3-oxoadipate enol-lactonase, at 2,282,279 to 2,283,058
_RS10690
RR42_RS10695: RR42_RS10695 - muconolactone delta-isomerase, at 2,283,121 to 2,283,405
_RS10695
RR42_RS10700: RR42_RS10700 - cyclase, at 2,283,462 to 2,284,508
_RS10700
Position (kb)
2282
2283
2284 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2281.295 kb on - strand, within RR42_RS10685 at 2281.363 kb on - strand, within RR42_RS10685 at 2281.400 kb on + strand, within RR42_RS10685 at 2281.401 kb on - strand, within RR42_RS10685 at 2281.429 kb on - strand, within RR42_RS10685 at 2281.611 kb on - strand at 2281.619 kb on + strand at 2281.620 kb on - strand at 2281.645 kb on - strand at 2281.691 kb on - strand at 2281.696 kb on + strand at 2281.886 kb on - strand at 2281.984 kb on + strand at 2282.016 kb on + strand at 2282.083 kb on + strand at 2282.083 kb on + strand at 2282.084 kb on - strand at 2282.085 kb on + strand at 2282.085 kb on + strand at 2282.136 kb on - strand at 2282.158 kb on - strand at 2282.158 kb on - strand at 2282.259 kb on - strand at 2282.259 kb on - strand at 2282.395 kb on - strand, within RR42_RS10690 at 2282.395 kb on - strand, within RR42_RS10690 at 2282.689 kb on + strand, within RR42_RS10690 at 2282.692 kb on - strand, within RR42_RS10690 at 2283.122 kb on + strand at 2283.179 kb on + strand, within RR42_RS10695 at 2283.179 kb on + strand, within RR42_RS10695 at 2283.180 kb on - strand, within RR42_RS10695 at 2283.186 kb on - strand, within RR42_RS10695 at 2283.210 kb on + strand, within RR42_RS10695 at 2283.257 kb on + strand, within RR42_RS10695 at 2283.258 kb on - strand, within RR42_RS10695 at 2283.258 kb on - strand, within RR42_RS10695 at 2283.405 kb on + strand at 2283.405 kb on + strand at 2283.405 kb on + strand at 2283.406 kb on - strand at 2283.406 kb on - strand at 2283.406 kb on - strand at 2283.584 kb on - strand, within RR42_RS10700 at 2283.692 kb on - strand, within RR42_RS10700 at 2283.696 kb on - strand, within RR42_RS10700 at 2283.696 kb on - strand, within RR42_RS10700 at 2283.763 kb on - strand, within RR42_RS10700 at 2283.763 kb on - strand, within RR42_RS10700 at 2283.847 kb on - strand, within RR42_RS10700 at 2283.910 kb on + strand, within RR42_RS10700 at 2283.911 kb on - strand, within RR42_RS10700
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,281,295 - RR42_RS10685 0.29 +0.7 2,281,363 - RR42_RS10685 0.44 +0.1 2,281,400 + RR42_RS10685 0.51 +0.3 2,281,401 - RR42_RS10685 0.52 +0.9 2,281,429 - RR42_RS10685 0.58 -0.3 2,281,611 - +0.8 2,281,619 + +0.1 2,281,620 - -1.1 2,281,645 - -0.4 2,281,691 - -0.1 2,281,696 + +0.6 2,281,886 - -0.1 2,281,984 + -0.1 2,282,016 + -0.3 2,282,083 + +1.3 2,282,083 + -0.0 2,282,084 - -0.8 2,282,085 + -1.1 2,282,085 + -0.1 2,282,136 - -1.9 2,282,158 - -0.6 2,282,158 - -0.9 2,282,259 - -0.1 2,282,259 - -0.4 2,282,395 - RR42_RS10690 0.15 -0.4 2,282,395 - RR42_RS10690 0.15 -2.1 2,282,689 + RR42_RS10690 0.53 -0.2 2,282,692 - RR42_RS10690 0.53 -0.6 2,283,122 + +1.9 2,283,179 + RR42_RS10695 0.20 -0.5 2,283,179 + RR42_RS10695 0.20 +2.3 2,283,180 - RR42_RS10695 0.21 -0.6 2,283,186 - RR42_RS10695 0.23 -0.9 2,283,210 + RR42_RS10695 0.31 +1.5 2,283,257 + RR42_RS10695 0.48 -0.5 2,283,258 - RR42_RS10695 0.48 -0.7 2,283,258 - RR42_RS10695 0.48 -1.8 2,283,405 + +0.1 2,283,405 + -1.0 2,283,405 + -0.4 2,283,406 - +1.9 2,283,406 - -1.5 2,283,406 - -0.8 2,283,584 - RR42_RS10700 0.12 -0.2 2,283,692 - RR42_RS10700 0.22 -0.6 2,283,696 - RR42_RS10700 0.22 -1.5 2,283,696 - RR42_RS10700 0.22 -0.4 2,283,763 - RR42_RS10700 0.29 -0.9 2,283,763 - RR42_RS10700 0.29 +0.5 2,283,847 - RR42_RS10700 0.37 -0.6 2,283,910 + RR42_RS10700 0.43 -0.6 2,283,911 - RR42_RS10700 0.43 +0.9
Or see this region's nucleotide sequence