Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10650 and RR42_RS10655 overlap by 4 nucleotides RR42_RS10655 and RR42_RS10660 overlap by 4 nucleotides
RR42_RS10650: RR42_RS10650 - acetyl-CoA synthetase, at 2,274,996 to 2,276,762
_RS10650
RR42_RS10655: RR42_RS10655 - pyruvate dehydrogenase, at 2,276,759 to 2,277,781
_RS10655
RR42_RS10660: RR42_RS10660 - pyruvate dehydrogenase, at 2,277,778 to 2,278,749
_RS10660
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.782 kb on - strand, within RR42_RS10650 at 2275.815 kb on + strand, within RR42_RS10650 at 2275.890 kb on + strand, within RR42_RS10650 at 2275.926 kb on + strand, within RR42_RS10650 at 2275.926 kb on + strand, within RR42_RS10650 at 2275.986 kb on + strand, within RR42_RS10650 at 2276.228 kb on - strand, within RR42_RS10650 at 2276.228 kb on - strand, within RR42_RS10650 at 2276.230 kb on + strand, within RR42_RS10650 at 2276.231 kb on - strand, within RR42_RS10650 at 2276.231 kb on - strand, within RR42_RS10650 at 2276.382 kb on + strand, within RR42_RS10650 at 2276.383 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.518 kb on + strand, within RR42_RS10650 at 2276.519 kb on - strand, within RR42_RS10650 at 2276.736 kb on - strand at 2276.816 kb on - strand at 2276.889 kb on - strand, within RR42_RS10655 at 2276.904 kb on + strand, within RR42_RS10655 at 2276.905 kb on - strand, within RR42_RS10655 at 2276.924 kb on + strand, within RR42_RS10655 at 2276.924 kb on + strand, within RR42_RS10655 at 2276.925 kb on - strand, within RR42_RS10655 at 2276.996 kb on + strand, within RR42_RS10655 at 2276.997 kb on - strand, within RR42_RS10655 at 2277.059 kb on + strand, within RR42_RS10655 at 2277.395 kb on + strand, within RR42_RS10655 at 2277.396 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.515 kb on + strand, within RR42_RS10655 at 2277.516 kb on - strand, within RR42_RS10655 at 2277.540 kb on - strand, within RR42_RS10655 at 2277.554 kb on + strand, within RR42_RS10655 at 2277.604 kb on + strand, within RR42_RS10655 at 2277.605 kb on - strand, within RR42_RS10655 at 2277.605 kb on - strand, within RR42_RS10655 at 2277.755 kb on + strand at 2277.889 kb on + strand, within RR42_RS10660 at 2277.936 kb on - strand, within RR42_RS10660 at 2278.062 kb on - strand, within RR42_RS10660 at 2278.078 kb on + strand, within RR42_RS10660 at 2278.086 kb on + strand, within RR42_RS10660 at 2278.118 kb on + strand, within RR42_RS10660 at 2278.122 kb on - strand, within RR42_RS10660 at 2278.186 kb on + strand, within RR42_RS10660 at 2278.187 kb on - strand, within RR42_RS10660 at 2278.245 kb on + strand, within RR42_RS10660 at 2278.246 kb on - strand, within RR42_RS10660 at 2278.307 kb on - strand, within RR42_RS10660 at 2278.307 kb on - strand, within RR42_RS10660 at 2278.326 kb on - strand, within RR42_RS10660 at 2278.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,275,773 + RR42_RS10650 0.44 -0.7 2,275,773 + RR42_RS10650 0.44 -0.7 2,275,773 + RR42_RS10650 0.44 -0.6 2,275,782 - RR42_RS10650 0.44 -0.3 2,275,815 + RR42_RS10650 0.46 +0.9 2,275,890 + RR42_RS10650 0.51 -1.5 2,275,926 + RR42_RS10650 0.53 -0.1 2,275,926 + RR42_RS10650 0.53 +0.1 2,275,986 + RR42_RS10650 0.56 +1.9 2,276,228 - RR42_RS10650 0.70 -0.5 2,276,228 - RR42_RS10650 0.70 -0.9 2,276,230 + RR42_RS10650 0.70 -0.1 2,276,231 - RR42_RS10650 0.70 -0.8 2,276,231 - RR42_RS10650 0.70 -0.6 2,276,382 + RR42_RS10650 0.78 +0.5 2,276,383 - RR42_RS10650 0.78 -1.5 2,276,386 - RR42_RS10650 0.79 +1.3 2,276,386 - RR42_RS10650 0.79 +0.2 2,276,386 - RR42_RS10650 0.79 -1.5 2,276,518 + RR42_RS10650 0.86 -0.8 2,276,519 - RR42_RS10650 0.86 +1.4 2,276,736 - -0.8 2,276,816 - +0.1 2,276,889 - RR42_RS10655 0.13 +1.8 2,276,904 + RR42_RS10655 0.14 +0.6 2,276,905 - RR42_RS10655 0.14 +0.4 2,276,924 + RR42_RS10655 0.16 -0.8 2,276,924 + RR42_RS10655 0.16 -0.1 2,276,925 - RR42_RS10655 0.16 -0.2 2,276,996 + RR42_RS10655 0.23 +1.1 2,276,997 - RR42_RS10655 0.23 +0.8 2,277,059 + RR42_RS10655 0.29 +0.2 2,277,395 + RR42_RS10655 0.62 +0.0 2,277,396 - RR42_RS10655 0.62 -0.6 2,277,401 - RR42_RS10655 0.63 +0.2 2,277,401 - RR42_RS10655 0.63 +0.4 2,277,401 - RR42_RS10655 0.63 -0.7 2,277,515 + RR42_RS10655 0.74 -1.9 2,277,516 - RR42_RS10655 0.74 +1.9 2,277,540 - RR42_RS10655 0.76 +1.7 2,277,554 + RR42_RS10655 0.78 -0.8 2,277,604 + RR42_RS10655 0.83 +0.3 2,277,605 - RR42_RS10655 0.83 -0.7 2,277,605 - RR42_RS10655 0.83 -0.3 2,277,755 + -0.5 2,277,889 + RR42_RS10660 0.11 +1.4 2,277,936 - RR42_RS10660 0.16 +0.5 2,278,062 - RR42_RS10660 0.29 +1.3 2,278,078 + RR42_RS10660 0.31 +2.9 2,278,086 + RR42_RS10660 0.32 -0.3 2,278,118 + RR42_RS10660 0.35 -0.5 2,278,122 - RR42_RS10660 0.35 -1.3 2,278,186 + RR42_RS10660 0.42 -0.6 2,278,187 - RR42_RS10660 0.42 -1.2 2,278,245 + RR42_RS10660 0.48 -0.6 2,278,246 - RR42_RS10660 0.48 +0.1 2,278,307 - RR42_RS10660 0.54 +0.5 2,278,307 - RR42_RS10660 0.54 +0.4 2,278,326 - RR42_RS10660 0.56 -0.1 2,278,673 - -0.6
Or see this region's nucleotide sequence