Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10140 and RR42_RS10145 are separated by 173 nucleotides RR42_RS10145 and RR42_RS10150 are separated by 112 nucleotides RR42_RS10150 and RR42_RS10155 overlap by 4 nucleotides
RR42_RS10140: RR42_RS10140 - 8-amino-7-oxononanoate synthase, at 2,173,619 to 2,174,842
_RS10140
RR42_RS10145: RR42_RS10145 - hypothetical protein, at 2,175,016 to 2,175,243
_RS10145
RR42_RS10150: RR42_RS10150 - hypothetical protein, at 2,175,356 to 2,175,637
_RS10150
RR42_RS10155: RR42_RS10155 - hypothetical protein, at 2,175,634 to 2,175,933
_RS10155
Position (kb)
2175
2176 Strain fitness (log2 ratio)
-1
0
1
2 at 2174.685 kb on + strand, within RR42_RS10140 at 2174.876 kb on + strand at 2174.876 kb on + strand at 2174.876 kb on + strand at 2174.876 kb on + strand at 2174.876 kb on + strand at 2174.877 kb on - strand at 2174.877 kb on - strand at 2174.877 kb on - strand at 2175.035 kb on + strand at 2175.035 kb on + strand at 2175.036 kb on - strand at 2175.036 kb on - strand at 2175.036 kb on - strand at 2175.163 kb on + strand, within RR42_RS10145 at 2175.163 kb on + strand, within RR42_RS10145 at 2175.164 kb on - strand, within RR42_RS10145 at 2175.164 kb on - strand, within RR42_RS10145 at 2175.211 kb on + strand, within RR42_RS10145 at 2175.211 kb on + strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.212 kb on - strand, within RR42_RS10145 at 2175.247 kb on - strand at 2175.247 kb on - strand at 2175.247 kb on - strand at 2175.296 kb on + strand at 2175.297 kb on - strand at 2175.297 kb on - strand at 2175.297 kb on - strand at 2175.300 kb on + strand at 2175.301 kb on - strand at 2175.301 kb on - strand at 2175.326 kb on + strand at 2175.326 kb on + strand at 2175.326 kb on + strand at 2175.327 kb on - strand at 2175.327 kb on - strand at 2175.327 kb on - strand at 2175.345 kb on + strand at 2175.345 kb on + strand at 2175.346 kb on - strand at 2175.358 kb on - strand at 2175.445 kb on - strand, within RR42_RS10150 at 2175.445 kb on - strand, within RR42_RS10150 at 2175.851 kb on + strand, within RR42_RS10155 at 2175.851 kb on + strand, within RR42_RS10155 at 2175.981 kb on + strand at 2176.021 kb on - strand at 2176.074 kb on - strand at 2176.074 kb on - strand at 2176.074 kb on - strand at 2176.216 kb on - strand at 2176.216 kb on - strand at 2176.216 kb on - strand at 2176.216 kb on - strand at 2176.227 kb on + strand at 2176.228 kb on - strand at 2176.346 kb on + strand at 2176.347 kb on - strand at 2176.483 kb on + strand at 2176.773 kb on - strand at 2176.773 kb on - strand at 2176.788 kb on + strand at 2176.789 kb on - strand at 2176.789 kb on - strand at 2176.789 kb on - strand at 2176.789 kb on - strand at 2176.904 kb on - strand at 2176.904 kb on - strand at 2176.909 kb on + strand at 2176.910 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 2,174,685 + RR42_RS10140 0.87 +0.0 2,174,876 + +1.9 2,174,876 + -1.7 2,174,876 + +0.3 2,174,876 + -1.4 2,174,876 + -0.6 2,174,877 - +0.1 2,174,877 - +0.7 2,174,877 - -1.4 2,175,035 + -0.7 2,175,035 + -0.3 2,175,036 - -0.1 2,175,036 - +0.9 2,175,036 - -0.9 2,175,163 + RR42_RS10145 0.64 -0.1 2,175,163 + RR42_RS10145 0.64 -0.1 2,175,164 - RR42_RS10145 0.65 +1.3 2,175,164 - RR42_RS10145 0.65 +0.9 2,175,211 + RR42_RS10145 0.86 +0.7 2,175,211 + RR42_RS10145 0.86 -1.0 2,175,212 - RR42_RS10145 0.86 +0.1 2,175,212 - RR42_RS10145 0.86 -0.0 2,175,212 - RR42_RS10145 0.86 -0.6 2,175,212 - RR42_RS10145 0.86 +0.4 2,175,212 - RR42_RS10145 0.86 -0.5 2,175,247 - +0.2 2,175,247 - -0.2 2,175,247 - +0.1 2,175,296 + +0.2 2,175,297 - -0.7 2,175,297 - -0.0 2,175,297 - +0.4 2,175,300 + -0.2 2,175,301 - -0.1 2,175,301 - +2.5 2,175,326 + -0.9 2,175,326 + -0.8 2,175,326 + -0.2 2,175,327 - +0.3 2,175,327 - +0.9 2,175,327 - +1.7 2,175,345 + -0.3 2,175,345 + +0.6 2,175,346 - +1.2 2,175,358 - -0.2 2,175,445 - RR42_RS10150 0.32 -1.0 2,175,445 - RR42_RS10150 0.32 -0.8 2,175,851 + RR42_RS10155 0.72 -1.1 2,175,851 + RR42_RS10155 0.72 +1.3 2,175,981 + +0.3 2,176,021 - -1.2 2,176,074 - -1.1 2,176,074 - +0.9 2,176,074 - -0.5 2,176,216 - +0.5 2,176,216 - +1.3 2,176,216 - +0.3 2,176,216 - +0.7 2,176,227 + +0.1 2,176,228 - -0.5 2,176,346 + -0.5 2,176,347 - -0.6 2,176,483 + -0.4 2,176,773 - -0.4 2,176,773 - -0.3 2,176,788 + +2.5 2,176,789 - -0.1 2,176,789 - -1.1 2,176,789 - +2.4 2,176,789 - +1.9 2,176,904 - +1.9 2,176,904 - -1.0 2,176,909 + +0.3 2,176,910 - -0.7
Or see this region's nucleotide sequence