Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09030 and RR42_RS09035 are separated by 296 nucleotides RR42_RS09035 and RR42_RS09040 are separated by 55 nucleotides RR42_RS09040 and RR42_RS09045 are separated by 248 nucleotides RR42_RS09045 and RR42_RS09050 are separated by 72 nucleotides RR42_RS09050 and RR42_RS09055 overlap by 14 nucleotides
RR42_RS09030: RR42_RS09030 - DNA-binding protein, at 1,898,036 to 1,900,447
_RS09030
RR42_RS09035: RR42_RS09035 - tRNA-Val, at 1,900,744 to 1,900,819
_RS09035
RR42_RS09040: RR42_RS09040 - tRNA-Asp, at 1,900,875 to 1,900,951
_RS09040
RR42_RS09045: RR42_RS09045 - peptidylprolyl isomerase, at 1,901,200 to 1,903,149
_RS09045
RR42_RS09050: RR42_RS09050 - arylesterase, at 1,903,222 to 1,903,917
_RS09050
RR42_RS09055: RR42_RS09055 - ABC transporter, at 1,903,904 to 1,904,602
_RS09055
Position (kb)
1901
1902
1903
1904 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1900.360 kb on + strand at 1900.470 kb on + strand at 1900.470 kb on + strand at 1900.471 kb on - strand at 1900.607 kb on + strand at 1900.607 kb on + strand at 1900.607 kb on + strand at 1900.626 kb on + strand at 1900.627 kb on - strand at 1900.653 kb on + strand at 1900.696 kb on + strand at 1900.896 kb on - strand, within RR42_RS09040 at 1901.092 kb on - strand at 1901.093 kb on + strand at 1901.093 kb on + strand at 1901.094 kb on - strand at 1901.094 kb on - strand at 1901.094 kb on - strand at 1901.152 kb on - strand at 1901.152 kb on - strand at 1901.152 kb on - strand at 1901.293 kb on + strand at 1901.293 kb on + strand at 1901.346 kb on + strand at 1901.419 kb on + strand, within RR42_RS09045 at 1901.617 kb on + strand, within RR42_RS09045 at 1901.617 kb on + strand, within RR42_RS09045 at 1901.617 kb on + strand, within RR42_RS09045 at 1901.618 kb on - strand, within RR42_RS09045 at 1901.854 kb on + strand, within RR42_RS09045 at 1901.854 kb on + strand, within RR42_RS09045 at 1901.855 kb on - strand, within RR42_RS09045 at 1901.855 kb on - strand, within RR42_RS09045 at 1901.857 kb on + strand, within RR42_RS09045 at 1901.908 kb on + strand, within RR42_RS09045 at 1902.245 kb on - strand, within RR42_RS09045 at 1902.289 kb on + strand, within RR42_RS09045 at 1902.403 kb on + strand, within RR42_RS09045 at 1902.432 kb on + strand, within RR42_RS09045 at 1902.433 kb on - strand, within RR42_RS09045 at 1902.439 kb on + strand, within RR42_RS09045 at 1902.440 kb on - strand, within RR42_RS09045 at 1902.440 kb on - strand, within RR42_RS09045 at 1902.440 kb on - strand, within RR42_RS09045 at 1902.724 kb on + strand, within RR42_RS09045 at 1902.724 kb on + strand, within RR42_RS09045 at 1902.725 kb on - strand, within RR42_RS09045 at 1902.725 kb on - strand, within RR42_RS09045 at 1902.726 kb on + strand, within RR42_RS09045 at 1902.727 kb on - strand, within RR42_RS09045 at 1902.727 kb on - strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.910 kb on + strand, within RR42_RS09045 at 1902.911 kb on - strand, within RR42_RS09045 at 1902.940 kb on + strand, within RR42_RS09045 at 1902.949 kb on + strand, within RR42_RS09045 at 1902.950 kb on - strand, within RR42_RS09045 at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.058 kb on - strand at 1903.223 kb on + strand at 1903.223 kb on + strand at 1903.224 kb on - strand at 1903.224 kb on - strand at 1903.231 kb on - strand at 1903.416 kb on - strand, within RR42_RS09050 at 1903.452 kb on - strand, within RR42_RS09050 at 1903.683 kb on + strand, within RR42_RS09050 at 1903.683 kb on + strand, within RR42_RS09050 at 1903.684 kb on - strand, within RR42_RS09050 at 1903.684 kb on - strand, within RR42_RS09050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 1,900,360 + -0.2 1,900,470 + -1.1 1,900,470 + -0.8 1,900,471 - -1.1 1,900,607 + -1.8 1,900,607 + +1.1 1,900,607 + +0.4 1,900,626 + -0.4 1,900,627 - +1.5 1,900,653 + -0.2 1,900,696 + +0.7 1,900,896 - RR42_RS09040 0.27 +1.0 1,901,092 - -0.3 1,901,093 + +0.5 1,901,093 + +1.0 1,901,094 - +0.2 1,901,094 - -0.4 1,901,094 - +0.4 1,901,152 - -0.6 1,901,152 - -1.4 1,901,152 - -0.0 1,901,293 + -1.5 1,901,293 + -1.4 1,901,346 + -0.6 1,901,419 + RR42_RS09045 0.11 -1.8 1,901,617 + RR42_RS09045 0.21 -1.0 1,901,617 + RR42_RS09045 0.21 -1.3 1,901,617 + RR42_RS09045 0.21 -0.4 1,901,618 - RR42_RS09045 0.21 -2.8 1,901,854 + RR42_RS09045 0.34 -0.5 1,901,854 + RR42_RS09045 0.34 +0.4 1,901,855 - RR42_RS09045 0.34 +1.5 1,901,855 - RR42_RS09045 0.34 -0.6 1,901,857 + RR42_RS09045 0.34 -0.0 1,901,908 + RR42_RS09045 0.36 -1.5 1,902,245 - RR42_RS09045 0.54 -0.9 1,902,289 + RR42_RS09045 0.56 +0.2 1,902,403 + RR42_RS09045 0.62 -0.0 1,902,432 + RR42_RS09045 0.63 +0.3 1,902,433 - RR42_RS09045 0.63 -1.9 1,902,439 + RR42_RS09045 0.64 -0.4 1,902,440 - RR42_RS09045 0.64 -1.8 1,902,440 - RR42_RS09045 0.64 -0.8 1,902,440 - RR42_RS09045 0.64 +0.2 1,902,724 + RR42_RS09045 0.78 -2.1 1,902,724 + RR42_RS09045 0.78 -0.4 1,902,725 - RR42_RS09045 0.78 -0.8 1,902,725 - RR42_RS09045 0.78 -0.4 1,902,726 + RR42_RS09045 0.78 +0.4 1,902,727 - RR42_RS09045 0.78 +2.0 1,902,727 - RR42_RS09045 0.78 +1.0 1,902,910 + RR42_RS09045 0.88 -1.2 1,902,910 + RR42_RS09045 0.88 -2.9 1,902,910 + RR42_RS09045 0.88 -1.6 1,902,910 + RR42_RS09045 0.88 +1.0 1,902,910 + RR42_RS09045 0.88 -1.3 1,902,911 - RR42_RS09045 0.88 -1.8 1,902,940 + RR42_RS09045 0.89 -2.0 1,902,949 + RR42_RS09045 0.90 -1.6 1,902,950 - RR42_RS09045 0.90 +0.0 1,903,058 - -0.8 1,903,058 - -1.6 1,903,058 - -1.5 1,903,058 - -0.4 1,903,058 - -2.4 1,903,058 - -0.6 1,903,223 + -0.0 1,903,223 + -0.6 1,903,224 - +0.4 1,903,224 - -1.8 1,903,231 - -0.5 1,903,416 - RR42_RS09050 0.28 -1.6 1,903,452 - RR42_RS09050 0.33 -1.5 1,903,683 + RR42_RS09050 0.66 -0.0 1,903,683 + RR42_RS09050 0.66 +0.8 1,903,684 - RR42_RS09050 0.66 -1.1 1,903,684 - RR42_RS09050 0.66 -0.3
Or see this region's nucleotide sequence