Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06400 and RR42_RS06405 overlap by 4 nucleotides RR42_RS06405 and RR42_RS06410 are separated by 49 nucleotides RR42_RS06410 and RR42_RS06415 are separated by 2 nucleotides RR42_RS06415 and RR42_RS06420 are separated by 341 nucleotides
RR42_RS06400: RR42_RS06400 - acetate permease, at 1,402,610 to 1,404,250
_RS06400
RR42_RS06405: RR42_RS06405 - membrane protein, at 1,404,247 to 1,404,609
_RS06405
RR42_RS06410: RR42_RS06410 - monooxygenase, at 1,404,659 to 1,405,882
_RS06410
RR42_RS06415: RR42_RS06415 - glyoxalase, at 1,405,885 to 1,406,373
_RS06415
RR42_RS06420: RR42_RS06420 - DSBA oxidoreductase, at 1,406,715 to 1,407,365
_RS06420
Position (kb)
1404
1405
1406 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1403.667 kb on + strand, within RR42_RS06400 at 1403.667 kb on + strand, within RR42_RS06400 at 1403.667 kb on + strand, within RR42_RS06400 at 1403.667 kb on + strand, within RR42_RS06400 at 1403.668 kb on - strand, within RR42_RS06400 at 1403.668 kb on - strand, within RR42_RS06400 at 1403.668 kb on - strand, within RR42_RS06400 at 1403.710 kb on - strand, within RR42_RS06400 at 1403.770 kb on - strand, within RR42_RS06400 at 1403.836 kb on - strand, within RR42_RS06400 at 1403.934 kb on + strand, within RR42_RS06400 at 1403.935 kb on - strand, within RR42_RS06400 at 1404.003 kb on + strand, within RR42_RS06400 at 1404.003 kb on + strand, within RR42_RS06400 at 1404.003 kb on + strand, within RR42_RS06400 at 1404.003 kb on + strand, within RR42_RS06400 at 1404.004 kb on - strand, within RR42_RS06400 at 1404.004 kb on - strand, within RR42_RS06400 at 1404.004 kb on - strand, within RR42_RS06400 at 1404.004 kb on - strand, within RR42_RS06400 at 1404.060 kb on + strand, within RR42_RS06400 at 1404.061 kb on - strand, within RR42_RS06400 at 1404.314 kb on + strand, within RR42_RS06405 at 1404.314 kb on + strand, within RR42_RS06405 at 1404.315 kb on - strand, within RR42_RS06405 at 1404.440 kb on + strand, within RR42_RS06405 at 1404.441 kb on - strand, within RR42_RS06405 at 1404.449 kb on + strand, within RR42_RS06405 at 1404.502 kb on + strand, within RR42_RS06405 at 1404.725 kb on - strand at 1404.762 kb on + strand at 1404.763 kb on - strand at 1404.763 kb on - strand at 1404.826 kb on - strand, within RR42_RS06410 at 1404.826 kb on - strand, within RR42_RS06410 at 1405.368 kb on + strand, within RR42_RS06410 at 1405.369 kb on - strand, within RR42_RS06410 at 1405.378 kb on - strand, within RR42_RS06410 at 1405.517 kb on + strand, within RR42_RS06410 at 1405.517 kb on + strand, within RR42_RS06410 at 1405.518 kb on - strand, within RR42_RS06410 at 1405.518 kb on - strand, within RR42_RS06410 at 1405.537 kb on - strand, within RR42_RS06410 at 1405.565 kb on + strand, within RR42_RS06410 at 1405.572 kb on + strand, within RR42_RS06410 at 1405.572 kb on + strand, within RR42_RS06410 at 1405.573 kb on - strand, within RR42_RS06410 at 1405.778 kb on + strand at 1405.778 kb on + strand at 1405.779 kb on - strand at 1405.779 kb on - strand at 1406.240 kb on + strand, within RR42_RS06415 at 1406.240 kb on + strand, within RR42_RS06415 at 1406.240 kb on + strand, within RR42_RS06415 at 1406.386 kb on + strand at 1406.387 kb on - strand at 1406.395 kb on - strand at 1406.517 kb on + strand at 1406.518 kb on - strand at 1406.551 kb on + strand at 1406.559 kb on + strand at 1406.560 kb on - strand at 1406.560 kb on - strand at 1406.560 kb on - strand at 1406.560 kb on - strand at 1406.560 kb on - strand at 1406.574 kb on - strand at 1406.682 kb on - strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.743 kb on - strand at 1406.835 kb on + strand, within RR42_RS06420 at 1406.836 kb on - strand, within RR42_RS06420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 1,403,667 + RR42_RS06400 0.64 +0.4 1,403,667 + RR42_RS06400 0.64 -0.8 1,403,667 + RR42_RS06400 0.64 -0.9 1,403,667 + RR42_RS06400 0.64 +0.2 1,403,668 - RR42_RS06400 0.64 -1.4 1,403,668 - RR42_RS06400 0.64 +1.5 1,403,668 - RR42_RS06400 0.64 +2.2 1,403,710 - RR42_RS06400 0.67 -1.1 1,403,770 - RR42_RS06400 0.71 -2.3 1,403,836 - RR42_RS06400 0.75 -0.6 1,403,934 + RR42_RS06400 0.81 -0.5 1,403,935 - RR42_RS06400 0.81 +0.6 1,404,003 + RR42_RS06400 0.85 -0.0 1,404,003 + RR42_RS06400 0.85 -0.0 1,404,003 + RR42_RS06400 0.85 -0.8 1,404,003 + RR42_RS06400 0.85 -1.0 1,404,004 - RR42_RS06400 0.85 -0.5 1,404,004 - RR42_RS06400 0.85 -0.5 1,404,004 - RR42_RS06400 0.85 -0.1 1,404,004 - RR42_RS06400 0.85 -1.5 1,404,060 + RR42_RS06400 0.88 +0.2 1,404,061 - RR42_RS06400 0.88 +2.2 1,404,314 + RR42_RS06405 0.18 +0.4 1,404,314 + RR42_RS06405 0.18 +0.6 1,404,315 - RR42_RS06405 0.19 -0.0 1,404,440 + RR42_RS06405 0.53 +2.1 1,404,441 - RR42_RS06405 0.53 +0.1 1,404,449 + RR42_RS06405 0.56 -0.8 1,404,502 + RR42_RS06405 0.70 -0.9 1,404,725 - +0.2 1,404,762 + -0.8 1,404,763 - +0.0 1,404,763 - -0.3 1,404,826 - RR42_RS06410 0.14 +0.1 1,404,826 - RR42_RS06410 0.14 +0.5 1,405,368 + RR42_RS06410 0.58 -0.5 1,405,369 - RR42_RS06410 0.58 +1.2 1,405,378 - RR42_RS06410 0.59 -1.1 1,405,517 + RR42_RS06410 0.70 +0.9 1,405,517 + RR42_RS06410 0.70 -0.2 1,405,518 - RR42_RS06410 0.70 -0.1 1,405,518 - RR42_RS06410 0.70 -0.6 1,405,537 - RR42_RS06410 0.72 -0.5 1,405,565 + RR42_RS06410 0.74 +0.2 1,405,572 + RR42_RS06410 0.75 -0.6 1,405,572 + RR42_RS06410 0.75 -1.8 1,405,573 - RR42_RS06410 0.75 -1.1 1,405,778 + +0.7 1,405,778 + -1.9 1,405,779 - +0.7 1,405,779 - -0.8 1,406,240 + RR42_RS06415 0.73 +2.2 1,406,240 + RR42_RS06415 0.73 -0.3 1,406,240 + RR42_RS06415 0.73 -0.7 1,406,386 + +1.9 1,406,387 - -0.3 1,406,395 - +0.7 1,406,517 + -0.8 1,406,518 - -0.3 1,406,551 + +0.2 1,406,559 + -0.4 1,406,560 - +0.0 1,406,560 - +1.0 1,406,560 - +0.3 1,406,560 - -0.3 1,406,560 - -1.9 1,406,574 - +0.5 1,406,682 - -1.3 1,406,742 + -1.0 1,406,742 + +0.2 1,406,742 + -0.1 1,406,742 + -2.5 1,406,742 + +2.2 1,406,743 - -1.1 1,406,835 + RR42_RS06420 0.18 -0.2 1,406,836 - RR42_RS06420 0.19 +0.6
Or see this region's nucleotide sequence