Experiment: R2A with Paraquat dichloride 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06260 and RR42_RS06265 are separated by 121 nucleotides RR42_RS06265 and RR42_RS06270 are separated by 185 nucleotides RR42_RS06270 and RR42_RS06275 are separated by 92 nucleotides RR42_RS06275 and RR42_RS06280 are separated by 95 nucleotides
RR42_RS06260: RR42_RS06260 - MBL fold metallo-hydrolase, at 1,373,314 to 1,374,102
_RS06260
RR42_RS06265: RR42_RS06265 - hypothetical protein, at 1,374,224 to 1,374,448
_RS06265
RR42_RS06270: RR42_RS06270 - cupin, at 1,374,634 to 1,375,761
_RS06270
RR42_RS06275: RR42_RS06275 - peptidylprolyl isomerase, at 1,375,854 to 1,376,387
_RS06275
RR42_RS06280: RR42_RS06280 - membrane protein, at 1,376,483 to 1,377,541
_RS06280
Position (kb)
1374
1375
1376 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1373.674 kb on + strand, within RR42_RS06260 at 1373.674 kb on + strand, within RR42_RS06260 at 1373.675 kb on - strand, within RR42_RS06260 at 1373.717 kb on + strand, within RR42_RS06260 at 1373.717 kb on + strand, within RR42_RS06260 at 1373.718 kb on - strand, within RR42_RS06260 at 1373.718 kb on - strand, within RR42_RS06260 at 1373.718 kb on - strand, within RR42_RS06260 at 1373.773 kb on + strand, within RR42_RS06260 at 1373.774 kb on - strand, within RR42_RS06260 at 1373.774 kb on - strand, within RR42_RS06260 at 1373.821 kb on - strand, within RR42_RS06260 at 1373.860 kb on + strand, within RR42_RS06260 at 1373.991 kb on + strand, within RR42_RS06260 at 1374.140 kb on + strand at 1374.147 kb on + strand at 1374.148 kb on - strand at 1374.477 kb on + strand at 1374.512 kb on + strand at 1374.546 kb on + strand at 1374.553 kb on + strand at 1374.596 kb on + strand at 1374.632 kb on + strand at 1374.636 kb on - strand at 1374.798 kb on - strand, within RR42_RS06270 at 1374.814 kb on - strand, within RR42_RS06270 at 1374.814 kb on - strand, within RR42_RS06270 at 1374.833 kb on + strand, within RR42_RS06270 at 1374.891 kb on - strand, within RR42_RS06270 at 1374.982 kb on - strand, within RR42_RS06270 at 1375.223 kb on + strand, within RR42_RS06270 at 1375.238 kb on + strand, within RR42_RS06270 at 1375.238 kb on + strand, within RR42_RS06270 at 1375.239 kb on - strand, within RR42_RS06270 at 1375.239 kb on - strand, within RR42_RS06270 at 1375.430 kb on + strand, within RR42_RS06270 at 1375.430 kb on + strand, within RR42_RS06270 at 1375.431 kb on - strand, within RR42_RS06270 at 1375.431 kb on - strand, within RR42_RS06270 at 1375.734 kb on + strand at 1375.824 kb on - strand at 1375.824 kb on - strand at 1375.824 kb on - strand at 1375.825 kb on + strand at 1375.826 kb on - strand at 1375.890 kb on + strand at 1375.890 kb on + strand at 1375.890 kb on + strand at 1375.890 kb on + strand at 1375.891 kb on - strand at 1375.891 kb on - strand at 1375.948 kb on - strand, within RR42_RS06275 at 1375.948 kb on - strand, within RR42_RS06275 at 1375.948 kb on - strand, within RR42_RS06275 at 1376.061 kb on + strand, within RR42_RS06275 at 1376.062 kb on - strand, within RR42_RS06275 at 1376.083 kb on + strand, within RR42_RS06275 at 1376.083 kb on + strand, within RR42_RS06275 at 1376.175 kb on + strand, within RR42_RS06275 at 1376.176 kb on - strand, within RR42_RS06275 at 1376.176 kb on - strand, within RR42_RS06275 at 1376.176 kb on - strand, within RR42_RS06275 at 1376.176 kb on - strand, within RR42_RS06275 at 1376.428 kb on + strand at 1376.485 kb on - strand at 1376.485 kb on - strand at 1376.559 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Paraquat dichloride 0.064 mg/ml remove 1,373,674 + RR42_RS06260 0.46 -0.8 1,373,674 + RR42_RS06260 0.46 -0.4 1,373,675 - RR42_RS06260 0.46 -2.4 1,373,717 + RR42_RS06260 0.51 +0.4 1,373,717 + RR42_RS06260 0.51 -0.0 1,373,718 - RR42_RS06260 0.51 +1.4 1,373,718 - RR42_RS06260 0.51 +1.4 1,373,718 - RR42_RS06260 0.51 +1.3 1,373,773 + RR42_RS06260 0.58 +1.8 1,373,774 - RR42_RS06260 0.58 +2.2 1,373,774 - RR42_RS06260 0.58 -1.1 1,373,821 - RR42_RS06260 0.64 -0.8 1,373,860 + RR42_RS06260 0.69 -1.4 1,373,991 + RR42_RS06260 0.86 -0.3 1,374,140 + -0.0 1,374,147 + -0.0 1,374,148 - -0.4 1,374,477 + -0.3 1,374,512 + -0.8 1,374,546 + -1.7 1,374,553 + +0.1 1,374,596 + -0.5 1,374,632 + -0.7 1,374,636 - -0.8 1,374,798 - RR42_RS06270 0.15 +0.4 1,374,814 - RR42_RS06270 0.16 +0.5 1,374,814 - RR42_RS06270 0.16 +0.3 1,374,833 + RR42_RS06270 0.18 +0.1 1,374,891 - RR42_RS06270 0.23 +1.7 1,374,982 - RR42_RS06270 0.31 +0.5 1,375,223 + RR42_RS06270 0.52 +0.2 1,375,238 + RR42_RS06270 0.54 +0.3 1,375,238 + RR42_RS06270 0.54 +1.2 1,375,239 - RR42_RS06270 0.54 +0.9 1,375,239 - RR42_RS06270 0.54 +0.6 1,375,430 + RR42_RS06270 0.71 -0.1 1,375,430 + RR42_RS06270 0.71 +1.2 1,375,431 - RR42_RS06270 0.71 +2.5 1,375,431 - RR42_RS06270 0.71 +1.3 1,375,734 + -1.0 1,375,824 - -1.1 1,375,824 - +1.3 1,375,824 - -0.4 1,375,825 + -0.1 1,375,826 - +0.1 1,375,890 + +0.2 1,375,890 + +0.6 1,375,890 + +0.2 1,375,890 + +2.2 1,375,891 - -0.7 1,375,891 - +0.2 1,375,948 - RR42_RS06275 0.18 +0.6 1,375,948 - RR42_RS06275 0.18 -0.8 1,375,948 - RR42_RS06275 0.18 -0.8 1,376,061 + RR42_RS06275 0.39 -0.3 1,376,062 - RR42_RS06275 0.39 -0.9 1,376,083 + RR42_RS06275 0.43 +0.2 1,376,083 + RR42_RS06275 0.43 -1.9 1,376,175 + RR42_RS06275 0.60 +0.8 1,376,176 - RR42_RS06275 0.60 -1.0 1,376,176 - RR42_RS06275 0.60 +2.2 1,376,176 - RR42_RS06275 0.60 -0.0 1,376,176 - RR42_RS06275 0.60 -1.1 1,376,428 + -0.1 1,376,485 - -0.8 1,376,485 - -0.6 1,376,559 + +0.3
Or see this region's nucleotide sequence